Human synovial MSCs were treated with 0.1% DMSO or 20 µM ABT-263 for a day. After the treatment, the cells were detached with trypsin, suspended in PBS, and the cell suspension was incubated with FITC-annexin V and propidium iodide (PI) using an FITC-Annexin V Apoptosis Detection Kit (BD Biosciences, NJ, USA). The fluorescence intensity was evaluated using a FACSVerse II system (BD Biosciences). The obtained data were analyzed using FlowJo version 8.7.1 (Ashland, OR, USA).
Facsverse 2 system
Manufactured by BD
The FACSVerse II system is a flow cytometry instrument designed for advanced cell analysis. It features a multi-laser configuration and a range of detection channels to enable comprehensive cellular characterization. The system is capable of high-throughput data acquisition and analysis, providing researchers with a powerful tool for their scientific investigations.
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2 protocols using facsverse 2 system
1
Annexin V-FITC and PI Apoptosis Assay
2
Characterization of Human Synovial MSCs
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Human synovial MSCs were detached with trypsin and suspended in PBS containing 2% FBS and 5 mM EDTA. The MSCs were then stained for 30 min at 4 °C with CD44-APCH7, CD73-V450, CD90-PE-Cy7, CD105-APC, CD34-PE, and CD45-PerCP-Cy5.5 antibodies (1:200; BD Biosciences), using Ghost Dye Violet (1:1000; Tonbo Biosciences, San Diego, CA, USA) to remove dead cells. Isotype controls were prepared as negative controls. The percentage of antigen-positive cells was evaluated using a FACSVerse II system (BD Biosciences).
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