α hog1 yc 20

Manufactured by Santa Cruz Biotechnology

The α-Hog1 (yC-20) is a primary antibody that recognizes the Hog1 protein, a mitogen-activated protein kinase (MAPK) involved in the osmoregulatory high osmolarity glycerol (HOG) response pathway in yeast. This antibody can be used to detect and study the Hog1 protein in various experimental applications.

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Lab products found in correlation

α phospho p38 t180 y182, by Cell Signaling Technology (2 mentions) α ha 16b12, by Fortrea (1 mentions) α myc, by Santa Cruz Biotechnology (1 mentions) Mouse anti goat, by Santa Cruz Biotechnology (1 mentions) α mpk1 yc 20, by Santa Cruz Biotechnology (1 mentions) α phospho p44 42 mapk thr202 tyr204, by Cell Signaling Technology (1 mentions) αha 16b12, by BioLegend (1 mentions)

2 protocols using α hog1 yc 20

Protein Detection via Immunoblotting

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Proteins were separated by SDS–PAGE (7.5% gels) followed by immunoblot analysis using mouse monoclonal α-Myc antibody (9E10; Santa Cruz), α-HA (16B12; Covance), α-GST (B-14; Santa Cruz), α-tetra His (Qiagen), or goat polyclonal α-Hog1 (yC-20; Santa Cruz) at a dilution of 1:10,000. Rabbit polyclonal α-phospho-p38 (T180/Y182, Cell Signaling) was used at a dilution of 1:2000 to detect phosphorylated Hog1. Secondary goat anti-mouse (Amersham) and donkey anti-goat (Santa Cruz) antibodies were used at a dilution of 1:10,000. Secondary donkey anti-rabbit (Amersham) was used at a dilution of 1:2000. All results involving immunoblot analyses were replicated at least once, and representative blots are shown.

Lee J, & Levin D.E. (2018). Intracellular mechanism by which arsenite activates the yeast stress MAPK Hog1. Molecular Biology of the Cell, 29(15), 1904-1915.

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Immunoblot Analysis of Yeast Proteins

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Proteins were separated by SDS-PAGE (10% gels) followed by immunoblot analysis using mouse monoclonal antibodies α-Myc (9E10; Santa Cruz), α-HA (16B12; BioLegend), α-Carboxypeptidase Y (CPY; 10A5B5; Abcam), or goat polyclonal α-Mpk1 (yC-20; Santa Cruz) at a dilution of 1:10,000. Rabbit polyclonal α-phospho-p44/42 MAPK (Thr202/Tyr204; Cell Signaling), was used at a dilution of 1:2,000 to detect phosphorylated Mpk1. Goat polyclonal α-Hog1 (yC-20; Santa Cruz) at a dilution of 1:10,000, or rabbit polyclonal α-phospho-p38 (T180/Y182, Cell Signaling) at a dilution of 1:2,000 were used to detect Hog1 and phosphorylated Hog1, respectively. Secondary goat anti-mouse (Jackson ImmunoResearch Labs), donkey anti-rabbit (GE Healthcare), or mouse anti-goat (Santa Cruz) antibodies were used at a dilution of 1:10,000. All results involving immunoblot analyses were replicated at least once and representative blots are shown.

Laz E.V., Lee J, & Levin D.E. (2019). Crosstalk between S. cerevisiae SAPKs Hog1 and Mpk1 is mediated by glycerol accumulation. Fungal biology, 124(5), 361-367.

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