Heat inactivated horse serum

Manufactured by Gemini Bio

Heat-inactivated horse serum is a laboratory reagent used to supplement cell culture media. It is prepared by heating horse serum to inactivate any potential pathogens.

Automatically generated - may contain errors

Lab products found in correlation

Penicillin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) F12 medium, by Merck Group (1 mentions) Hepes, by Thermo Fisher Scientific (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions) Dmem f12, by Thermo Fisher Scientific (1 mentions) Accutase, by GE Healthcare (1 mentions)

2 protocols using heat inactivated horse serum

Isolation of Primary Neonatal Microglia

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primary neonatal microglia were collected as previously described [16 (link)]. Briefly, mixed glial cell cultures were maintained in PDL-coated T75 or T175 flasks (Genesee Scientific, San Diego, CA) in D10C media: DMEM (Sigma-Aldrich, St. Louis, MO) supplemented with 10% heat-inactivated horse serum (Gemini Bio-Products, West Sacramento, CA), 10% F12 medium (Sigma), 2 mM L-glutamine (Gibco/Thermo Fisher Scientific), 5 mM HEPES (Gibco/Thermo Fisher Scientific), 100 IU/mL penicillin and 100 μg/mL streptomycin (Gibco/Thermo Fisher Scientific) for up to four weeks. Starting on week 2 and every week thereafter, floating and loosely adherent microglia were harvested by manual agitation.

Fung S., Smith C.L., Prater K.E., Case A., Green K., Osnis L., Winston C., Kinoshita Y., Sopher B., Morrison R.S., Garden G.A, & Jayadev S. (2020). Early-Onset Familial Alzheimer Disease Variant PSEN2 N141I Heterozygosity is Associated with Altered Microglia Phenotype. Journal of Alzheimer's Disease, 77(2), 675-688.

+ Open protocol

+ Expand

Cryopreservation and Culturing of Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were pelleted by centrifugation at 835 × g for 15 min at 4 °C, washed in Hank's buffered saline solution, frozen in a viable state in DMSO Freeze media (Invitrogen; Gaithersburg, MD), and stored in liquid nitrogen until used. Frozen cells were maintained in DMEM/F12 (Invitrogen) supplemented with 5% heat-inactivated horse serum (Gemini BioProducts, W. Sacramento, CA) and 100 units/ml penicillinstreptomycin. Cells were passaged by trypsinization. All cells were incubated in a 5% carbon dioxide atmosphere at 37 °C. The culture medium was changed every second day, and cells were passaged every 2-4 days using accutase (PAA Laboratories GmbH, Linz, Austria).

Kappos E.A., Engels P.E., Tremp M., Sieber P.K., von Felten S., Madduri S., Meyer Zu Schwabedissen M., Fischmann A., Schaefer D.J, & Kalbermatten D.F. (2018). Denervation leads to volume regression in breast cancer. Journal of plastic, reconstructive & aesthetic surgery : JPRAS, 71(6).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!