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Pen strep

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Pen/Strep is a sterile solution containing penicillin and streptomycin, two common antibiotics used in cell culture applications. It is designed to prevent bacterial contamination in cell culture media and samples.

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Lab products found in correlation

Fetal bovine serum (fbs), by Omega Scientific (4 mentions) Bovine pituitary extract, by Thermo Fisher Scientific (2 mentions) Insulin, by Thermo Fisher Scientific (2 mentions) Recombinant human egf, by BD (2 mentions) Charcoal dextran treated fbs, by BD (2 mentions) Dulbecco modified eagle medium (dmem), by Merck Group (2 mentions) Rpmi 1640 media, by Merck Group (2 mentions) Clone e6 1, by American Type Culture Collection (1 mentions) Rpmi 1640, by Omega Scientific (1 mentions) Hepes, by Omega Scientific (1 mentions) Primary human hepatocytes, by Thermo Fisher Scientific (1 mentions) Lx 2 cells, by Merck Group (1 mentions) Hera cell 5 co2 incubator, by Thermo Fisher Scientific (1 mentions) Hepg2 and hek293 cells, by American Type Culture Collection (1 mentions) Hepatocyte plating supplement pack, by Thermo Fisher Scientific (1 mentions) Collagen coated 24 well plates, by Thermo Fisher Scientific (1 mentions) T75 flask, by Corning (1 mentions) Epidermal growth factor (egf), by Lonza (1 mentions) Rpmi 1640, by American Type Culture Collection (1 mentions) Mol l dihydrotestosterone, by Merck Group (1 mentions)

5 protocols using pen strep

1

Cell Culture Protocols for LX-2, HepG2, HEK293, and Primary Hepatocytes

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LX-2 cells (Merck Millipore; Billerica, MA) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Thermo Fisher Scientific; Waltham, MA) supplemented with 2% fetal bovine serum (FBS) and 1% Pen/Strep (Omega Scientific; Tarzana, CA). Approximately 1 x 106 cells were thawed in T-75 flasks (Corning Life Sciences; Corning, NY) containing 12 mL cell culture medium and placed at 37°C in a Hera Cell 5% CO2 incubator (Thermo Fisher Scientific). Culture medium was replaced the first day after thawing, and then every 72 hours until 80% confluent. HepG2 and HEK293 cells (ATCC; Manassas, VA) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) and 1% Pen/Strep (Omega Scientific). Culture medium was replaced the first day after thawing, and then every 48 hours until 80% confluent. Primary human hepatocytes (Thermo Fisher Scientific) were thawed in 50 mL Cryopreserved Hepatocyte Recovery Medium (CHRM) and plated in 500 υL William's E Medium supplemented with Hepatocyte Plating Supplement Pack on collagen-coated 24-well plates (Thermo Fisher Scientific). Culture medium was replaced the first day after thawing with William's E Medium supplemented with Hepatocyte Maintenance Supplement Pack.
Gerhard G.S., Hanson A., Wilhelmsen D., Piras I.S., Still C.D., Chu X., Petrick A.T, & DiStefano J.K. (2019). AEBP1 expression increases with severity of fibrosis in NASH and is regulated by glucose, palmitate, and miR-372-3p. PLoS ONE, 14(7), e0219764.
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2

Establishment and Culture of Prostate Cell Line

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The PKV cell line was generated by FACS sorting CD45-CD31-Ter119-EpCAM+GFP- epithelial cells from the prostates of 10 week old CPKV mice and culturing them in 0.2% gelatin-coated 10 cm dishes with Dulbecco’s modified eagle medium (DMEM) (Sigma-Aldrich, St. Louis, MO, USA) containing 1% Pen/Strep, 10% Fetal Bovine Serum (FBS) (Omega Scientific, Tarzana, CA, USA), 25 μg /mL bovine pituitary extract, 5 μg /mL insulin (Invitrogen, Grand Island, NY, USA), and 6 ng/mL recombinant human EGF (BD Biosciences, San Jose, CA, USA). LNCaP cells were grown in RPMI-1640 media (Sigma-Aldrich) with 1% Pen/Strep and 10% FBS. For studies carried out in the context of androgen deprivation, media containing 10% charcoal dextran-treated (CDT) FBS (BD Biosciences) was used.
Ruscetti M., Dadashian E.L., Guo W., Quach B., Mulholland D.J., Park J.W., Tran L.M., Kobayashi N., Bianchi-Frias D., Xing Y., Nelson P.S, & Wu H. (2015). HDAC Inhibition Impedes Epithelial-Mesenchymal Plasticity and Suppresses Metastatic, Castration-Resistant Prostate Cancer. Oncogene, 35(29), 3781-3795.
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3

Retroviral Transduction of CD28 Mutants

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The entire coding region of CD28 with or without D124V or T195P mutations was cloned into GFP-containing pMIG vectors (Promega, Madison, WI). Retrovirus production in 293T cells and infection of ~500,000 Jurkat cells, clone E6.1 (ATCC) were performed essentially as described35 (link). Cells were confirmed free of mycoplasma with the Universal Mycoplasma Contamination Kit (ATCC). Transduced cells stably maintaining GFP expression after one week were sorted for equivalent GFP expression (Supplemental Table 4) using flow cytometry. Cells were cultured in RPMI-1640, 10% FBS (Omega Scientific, Tarzana, CA), 1% Pen/Strep, 1% 1M HEPES and frozen or used in the activation assay. All cell culture reagents were obtained from Life Technologies (Waltham, MA) unless otherwise stated. The in vitro assays for T-cell stimulation and NF-κB luciferase assay are described in detail in the supplemental section.
Rohr J., Guo S., Huo J., Bouska A., Lachel C., Li Y., Simone P.D., Zhang W., Gong Q., Wang C., Cannon A., Heavican T., Mottok A., Hung S., Rosenwald A., Gascoyne R., Fu K., Greiner T.C., Weisenburger D.D., Vose J.M., Staudt L.M., Xiao W., Borgstahl G.E., Davis S., Steidl C., McKeithan T., Iqbal J, & Chan W.C. (2015). Recurrent activating mutations of CD28 in peripheral T-cell lymphomas. Leukemia, 30(5), 1062-1070.
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4

Prostate Cell Line Culture Conditions

Cited 2 times
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Prostate cell lines BPH1, (LNCaP (ATCC), and LNCaP-C4 (27 (link))) were cultured in 5% heat-inactivated FBS (Sigma), 100 μg/mL penicillin-steptomycin (pen-strep) (Gibco), 1X HEPES (Gibco), 1X RPMI 1640 (CellGro) with 10-8 Mol/L dihydrotestosterone (Sigma) or in RPMI 1640 (ATCC) 10% FBS (Omega Scientific) with 100μg/mL pen-strep. UGSM-2 and Gli3-/-(18 (link), 28 (link)) cells were cultured in 10% heat-inactivated FBS (Sigma), 100 μg/ml pen-strep (CellGro), 1X ITS (Lonza) with 10-8 Mol/L dihydrotestosterone (Sigma). BHPrE1 and NHPrE1 cells were cultured in DMEM/F12 5% FBS, 0.4% bovine pituitary extract (Hammnod Cell Tech, Windsor, CA), 0.005 μg/mL epidermal growth factor, 1 μg/mL ITS (Lonza), and 100 μg/mL pen-strep (29 (link)). P2 cells were cultured as previously described (30 (link)). For reduced serum experiments, 1% heat-inactivated FBS was used with the appropriate media.
Powers G.L., Hammer K.D., Domenech M., Frantskevich K., Malinowski R.L., Bushman W., Beebe D.J, & Marker P.C. (2014). Phosphodiesterase 4D Inhibitors Limit Prostate Cancer Growth Potential. Molecular cancer research : MCR, 13(1), 149-160.
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5

Establishment and Culture of Prostate Cell Line

Check if the same lab product or an alternative is used in the 5 most similar protocols
The PKV cell line was generated by FACS sorting CD45-CD31-Ter119-EpCAM+GFP- epithelial cells from the prostates of 10 week old CPKV mice and culturing them in 0.2% gelatin-coated 10 cm dishes with Dulbecco’s modified eagle medium (DMEM) (Sigma-Aldrich, St. Louis, MO, USA) containing 1% Pen/Strep, 10% Fetal Bovine Serum (FBS) (Omega Scientific, Tarzana, CA, USA), 25 μg /mL bovine pituitary extract, 5 μg /mL insulin (Invitrogen, Grand Island, NY, USA), and 6 ng/mL recombinant human EGF (BD Biosciences, San Jose, CA, USA). LNCaP cells were grown in RPMI-1640 media (Sigma-Aldrich) with 1% Pen/Strep and 10% FBS. For studies carried out in the context of androgen deprivation, media containing 10% charcoal dextran-treated (CDT) FBS (BD Biosciences) was used.
Ruscetti M., Dadashian E.L., Guo W., Quach B., Mulholland D.J., Park J.W., Tran L.M., Kobayashi N., Bianchi-Frias D., Xing Y., Nelson P.S, & Wu H. (2015). HDAC Inhibition Impedes Epithelial-Mesenchymal Plasticity and Suppresses Metastatic, Castration-Resistant Prostate Cancer. Oncogene, 35(29), 3781-3795.
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