1 1 2 2 tetraoleoyl cardiolipin tocl

Manufactured by Avanti Polar Lipids

Sourced in United States

1,1′,2,2′-tetraoleoyl-cardiolipin (TOCL) is a synthetic phospholipid. It is a tetraester of the phospholipid cardiolipin, where all four hydroxyl groups are esterified with oleic acid. TOCL is used as a laboratory research tool.

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Lab products found in correlation

1 2 dioleoyl sn glycero 3 phosphocholine dopc, by Avanti Polar Lipids (2 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Amplex red, by Thermo Fisher Scientific (1 mentions) Dioleoylphosphatidylcholine dopc, by Avanti Polar Lipids (1 mentions) Dioleoylphosphatidylserine dops, by Avanti Polar Lipids (1 mentions) Dioleoylphosphatidylethanolamine dope, by Avanti Polar Lipids (1 mentions) 2 2 azino bis, by Merck Group (1 mentions)

3 protocols using 1 1 2 2 tetraoleoyl cardiolipin tocl

Cytochrome c Oxidase Activity Assay

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Horse heart cyt c (type C-7752, >95%), diethylenetriaminepentaacetic acid (DTPA), H₂O_2, and fetal bovine serum (FBS) were purchased from Sigma-Aldrich (St. Louis, MO). 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1,1′,2,2′-tetraoleoyl-cardiolipin (TOCL) were purchased from Avanti Polar Lipids (Alabaster, AL). Amplex Red (N-acetyl-3,7-dihydroxyphenoxazine) was obtained from Life Technologies (Carlsbad, CA).

Jiang J., Bakan A., Kapralov A.A., Silva K.I., Huang Z., Amoscato A.A., Peterson J., Garapati V.K., Saxena S., Bayir H., Atkinson J., Bahar I, & Kagan V.E. (2014). Designing Inhibitors of Cytochrome c/Cardiolipin Peroxidase Complexes: Mitochondria-Targeted Imidazole-Substituted Fatty Acids. Free radical biology & medicine, 71, 221-230.

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Lipid Standards Procurement and Characterization

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All reagents used were purchased from Sigma Chemical Co. (St. Louis, MO). Phospholipid standards including dioleoylphosphatidylserine (DOPS), dioleoylphosphatidyl-ethanolamine (DOPE), dioleoylphosphatidylcholine (DOPC) and 1,1’,2,2’-tetraoleoylcardiolipin (TOCL) were purchased from Avanti Polar Lipids (Alabaster, AL, USA).

Stoyanovsky D.A., Sparvero L.J., Amoscato A.A., He R.R., Watkins S., Pitt B.R., Bayir H, & Kagan V.E. (2014). Improved Spatial Resolution of MALDI Imaging of Lipids in the Brain by Alkylated Derivatives of 2,5-Dihydroxybenzoic Acid. Rapid communications in mass spectrometry : RCM, 28(5), 403-412.

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Peroxidase Assay of Oxidized hCc Variants

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Peroxidase assays of the oxidised hCc variants were carried out in the absence and presence of liposomes using H 2 O 2 (Sigma-Aldrich) and 2,2-Azinobis(3-ethylbenthiazoline-6-sulfonic acid) (ABTS) (Sigma-Aldrich). The phospholipids 1,1',2,2'-tetraoleoyl cardiolipin (TOCL) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) (AVANTI Polar Lipids, USA) were mixed in a 1:1 ratio and vortexed with the appropriate amount of buffer (20 mM sodium phosphate pH 6.5) and sonicated for 5 min in a high power sonicating water bath filled with ice-cold water to give the desired stock concentration as previously described 30

Deacon O.M., Svistunenko D.A., Moore G.R., Wilson M.T, & Worrall J.A.R. (2018). Naturally Occurring Disease-Related Mutations in the 40-57 Ω-Loop of Human Cytochrome c Control Triggering of the Alkaline Isomerization. Biochemistry, 57(29).

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