A549 cells (a human lung adenocarcinoma cell line) and H2170 cells (squamous cell carcinoma cell lines) were purchased from American Type Culture Collection (USA) and PC9 cells (a human lung adenocarcinoma cell line) was purchased from Immuno‐Biological Laboratories Co. (Japan). H226 cells (human lung squamous cell carcinoma cell lines) were obtained from Dr J. D. Minna (University of Texas Southwestern Medical School, USA) and Dr M. Akiyama (Radiation Effects Research Foundation, Japan), respectively. AB1‐HA cells (a murine malignant mesothelioma cell line), a transfectant with the gene encoding influenza HA into an AB1 cells, were purchased from Public Health England (UK). Tumor cell lines were maintained in DMEM supplemented with 10% heat‐inactivated fetal bovine serum (FBS), penicillin (100 U/mL) and streptomycin (50 μg/mL). All cells were cultured at 37°C in a humidified atmosphere of 5% CO2 in air.
H2170 cells
Manufactured by American Type Culture Collection
Sourced in United States
H2170 cells are a lung cancer cell line derived from a human small cell lung carcinoma. They are adherent cells that can be used for in vitro studies.
Automatically generated - may contain errors
Lab products found in correlation
Pc 9 cells, by Immuno-Biological Laboratories (1 mentions)
A549 cell, by American Type Culture Collection (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Antibiotic antimycotic, by Thermo Fisher Scientific (1 mentions)
2 protocols using h2170 cells
1
Lung Cancer and Mesothelioma Cell Lines
2
Microscopic Imaging of Diverse Cell Types
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To verify the functionality of microscopic system for cells/tissues imaging, various biological samples were tested. They include (i) plant cell imaging: grape skin (exocarp) and grape flesh (mesocarp); and (ii) animal cell imaging: human RBCs and lung cancer cells (H2170 cell line). Image focusing was done by manual adjustment of the sample stage, with other optical components fixed.
RBCs: To prevent RBC aggregation that could obscure imaging quality assessment, 10 μl of RBCs was diluted to 0.9X concentration with 15 μl deionized water and 75 μl PBS-EDTA-BSA mixture. PBS was used to prevent osmotic cellular rupture. EDTA functioned as an anti-clotting agent of RBCs.
H2170: The H2170 cells (from American Type Culture Collection (ATCC)) were cultured in the tissue culture flasks (surface area of 75cm2) (TPP), which was placed in a CO2 incubator with 5% CO2 under 37 °C. The full culture medium was ATCC modified RPMI-1640 (Gibco) supplemented with 10% fetal bovine serum (FBS) (Gibco) and 1% antibiotic–antimycotic (Gibco). Depending on cell confluency observed by a standard light microscope, passage or medium replacement was performed 2–3 times each week.
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