Raji (Burkitt’s lymphoma), Daudi (Burkitt’s lymphoma), JeKo-1 (mantle cell lymphoma), RL (DLBCL), DOHH-2 (DLBCL), Toledo (DLBCL), MV-4-11 (AML), and THP-1 (AML) cell lines were obtained from ATCC, authenticated by Regeneron, and tested negative for Mycoplasma. All experiments were conducted with low-passage (1-6) cell cultures.
Dohh2
Manufactured by American Type Culture Collection
Sourced in United States
The DOHH2 is a cell line derived from a human diffuse large B-cell lymphoma. It is used for research purposes in the study of lymphoma and related diseases.
Automatically generated - may contain errors
Lab products found in correlation
Su dhl 6, by American Type Culture Collection (3 mentions)
Oci ly10, by American Type Culture Collection (2 mentions)
Thp 1, by American Type Culture Collection (2 mentions)
Wsu dlcl2, by American Type Culture Collection (2 mentions)
Karpas422, by American Type Culture Collection (2 mentions)
Su dhl 4, by American Type Culture Collection (2 mentions)
U2932, by American Type Culture Collection (2 mentions)
Penicillin, by Thermo Fisher Scientific (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Atlanta Biologicals (2 mentions)
Mv4 11, by American Type Culture Collection (1 mentions)
Ahh 1, by American Type Culture Collection (1 mentions)
Fetal calf serum (fcs), by Merck Group (1 mentions)
Histone h3 antibody 9715, by Cell Signaling Technology (1 mentions)
Su dhl 4, by Merck Group (1 mentions)
Su dhl 6, by Merck Group (1 mentions)
Hbl 1, by American Type Culture Collection (1 mentions)
Pfeiffer, by American Type Culture Collection (1 mentions)
Roswell park memorial institute (rpmi), by Merck Group (1 mentions)
Roswell park memorial institute (rpmi), by Thermo Fisher Scientific (1 mentions)
9 protocols using dohh2
1
Profiling Hematological Cell Lines
2
Isolation and Characterization of B-cell Lymphoma Cell Lines
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Human B-cell lymphoma cells DOHH2 and OCI-LY10 (BNCC338032 and BNCC337742) and human B lymphocyte AHH-1 (ATCC No. CRL-8146) were purchased from BeNa Culture Collection and ATCC core collection, respectively. AHH-1 was collected from the peripheral blood of a 33 years old human of Caucasian ethnicity. DOHH2 was cultured in 90% high-sugar Dulbecco’s modified eagle medium (DMEM) containing 4mmL of glutamine and sodium pyruvate and 10% fetal bovine serum (FBS), and AHH-1 and OCI-LY10 were cultured in 90% Roswell Park Memorial Institute-1640 (RPMI-1640) containing 10% FBS. The cells were all cultured under 95% air + 5% carbon dioxide at 37°C. The purchased cells were used after 2–3 times of passage. Cells at the logarithmic growth phase were collected and lysed with TRIzol lysate, and then the total RNA was extracted from the cells with chloroform, isopropanol, and ethanol in order. The purity, concentration, and integrity of the total RNA were determined using ultraviolet spectrophotometry and agarose gel electrophoresis. It was required that the ratio of these factors at 28s to these factors at 18s was larger than or equal to 2, and the ratio of A260/A280 was between 1.8 and 2.1.
3
Cell Culture and Western Blotting Protocol for Lymphoma Cell Lines
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Cell lines were cultured in RPMI (Invitrogen) with 10% fetal calf serum (Sigma-Aldrich), except for SU-DHL-4 and SU-DHL-6 which were cultured in RPMI with 20% fetal calf serum, and OCI-Ly10 which was cultured in Iscove‘s modified Dulbecco medium with 10% fetal calf serum. All cell lines were maintained at 37 °C. SU-DHL-4, SU-DHL-6, Karpas422, DOHH-2 and WSU-DLCL2 were purchased from DSZM, Pfeiffer was purchased from ATCC and OCI-Ly10 and HBL-1 were gifts from the Weng lab (BCCRC) to the LCR lab. All cell lines were authenticated by STR profiling. SU-DHL-6. HBL-1 and WSU-DLCL2 were not mycoplasma tested but all others tested negative.
Western Blotting was performed as described26 (link) using the Rabbit Polyclonal IκBζ Antibody (TA336346) (Origene) (dilution 1:500) and the Histone H3 Antibody #9715 (Cell Signaling) (dilution 1:1000). Un-cropped western blot is shown in Supplementary Figure11 .
Western Blotting was performed as described26 (link) using the Rabbit Polyclonal IκBζ Antibody (TA336346) (Origene) (dilution 1:500) and the Histone H3 Antibody #9715 (Cell Signaling) (dilution 1:1000). Un-cropped western blot is shown in Supplementary Figure
4
Culturing 16 DLBCL Cell Lines
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The 16 DLBCL cell lines (DOHH2, HT, OCI-LY19, DB, OCI-LY1, SUDHL-4, SUDHL-5, SUDHL-10, NUDHL-1, OCI-LY7, WSU-DLCL2, SUDHL-6, NUDUL-1, U2932, OCI-LY3, and RI-1) were purchased from the American Type Culture Collection or from DSMZ (Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Germany). They were grown in RPMI-1640 Glutamax medium (Gibco, Invitrogen, Cergy Pontoise, France), supplemented with 10% fetal bovine serum (FBS) (PAA laboratory GmbH, Pasching, Austria) (U2932, SUDHL-4, HT, DOHH2, SUDHL-10, RI-1, and WSU-DLCL2 cells), 20% FBS (OCI-LY3, DB, SUDHL-5, NUDHL-1, and SUDHL-6 cells), or 15% FBS (NUDUL-1 cells). OCI-LY1, and OCI-LY7 cells were cultured in IMDM Glutamax (Gibco, Invitrogen, Cergy Pontoise, France), supplemented with 20% FBS, and OCI-LY19 cells in MEM alpha modified Glutamax (Gibco, Invitrogen, Cergy Pontoise, France) with 20% FBS. Cultures were maintained at 37 °C in a humidified atmosphere with 5% CO2. Contamination by Mycoplasma species was regularly monitored.
5
Establishing Drug-Resistant Lymphoma Cell Lines
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The FL cell lines, DOHH2 and RL, were obtained from American Type Culture Collection (ATCC; Manassas, VA, USA) and Leibniz Institute‐DSMZ (Braunschweig, Germany), respectively. The splenic MZL cell line SLVL was from RIKEN BioResource Center (Ibaraki, Japan), and the NK‐92 cell line was from ATCC. All cell lines were maintained in RPMI medium [RPMI‐1640 with 10% fetal bovine serum (FBS), supplemented with 2 mmol/L L‐glutamine, 1% penicillin/streptomycin and 1 mmol/L sodium pyruvate]. DOHH2 and RL cells were made resistant to bendamustine, 4 hydroperoxycyclophosphamide (4‐HC), and doxorubicin by stepwise exposure to increasing drug concentrations up to 100, 5 and 250 μmol/l, respectively.
6
Cell Lines for B-Cell Lymphoma Research
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Diffuse Large B-Cell lines DHL-6, DHL-4, DHL-10, Karpas-422, RL, TMD-8, U2932, and RIVA; DHL cell lines ROS-50, DOHH-2 and VAL; and BL cell lines Ramos, Daudi and Raji were obtained from American Type Culture Collection (Rockville, MD). The rituximab resistant cell lines, RL 4RH, U2932 4RH and Raji 4RH was developed in the Hernandez Laboratory (27) . OCI-LY1 was a gift from the Lou Staudt Lab. All cell lines were maintained in RPMI 1640 (Thermo Fisher, Grand Island, NY) supplemented with 10% heat-inactivated fetal bovine serum (Atlanta Biologicals, Norcross, GA), 5mM HEPES, 100U/mL penicillin and 100μg/mL streptomycin (Thermo Fisher, Grand Island, NY) at 37°C and 5%CO 2 .
7
Cytotoxic NK-92 Cell Assay
8
Characterization of Leukemic Cell Lines
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Human leukemic cell lines SU-DHL-6, OCL-LY-19, SU-DHL-16 (DLBCL), and DOHH2 (FL) were obtained from Dr. Eric Hsi and Dr. Neetu Gupta (Cleveland Clinic), Nalm6, Reh (acute lymphoblastic leukemia) were purchased from the American Type Culture Collection (Manassas, VA, USA), Mec-2 from Dr. Y. Saunthararajah (Cleveland Clinic) and MO1040 (both CLL) from Dr. Riccardo Dalla-Favera (Columbia University) and used as before.32 (link), 33 (link), 45 (link) All cell lines were cultured in RPMI-1640 medium supplemented with 10% FBS (Atlanta Biologicals, Lawrenceville, GA, USA), and antibiotic-antimycotic (Gibco, Life Technologies, Gaithersburg, MD, USA). ABT199-R cells were cultured with 5% FBS. Cell lines were routinely screened for Mycoplasma, variations in growth rates, changes in morphological characteristics, and their response to stress with Annexin V FITC-PI staining; their passage number did not exceed 20. ABT-737 and ABT-199 were obtained from AbbVie (Chicago, IL, USA); NVP-BEZ235, RAD001, and GS-1101 from Selleck Chemicals (Houston, TX, USA); and verapamil and cycloheximide from Sigma-Aldrich (St. Louis, MO, USA).
9
Cell Culture Protocol for Various Cell Types
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HeLa, HEK293T, THP-1, Jurkat, CHO, NIH3T3, CA46, Balb3T3, HT2, KMS-12, DOHH2, REC-1, HCC 78 and NCI-H196 cells were obtained from American Type Culture Collection (Manassas, VA, USA) and cultured following the manufacturer's instructions. CD34+ cells were cultured and used for cell delivery purpose at CETC-Hôpital Rosemont (Montreal, QC, Canada). MSCs, ESCs and iPSCs were cultured and used for cell delivery purpose at CCRM (Toronto, ON, CAN). Myoblasts are primary human cells kindly provided by Professor J.P. Tremblay (Université Laval, Quebec, QC, Canada). Normal Peripheral Blood CD56+ NK cells are natural killer cells obtained from Allcells Inc. (Alameda, CA, USA). Cortical cells are primary cells extracted from rat brain and cultured and used for cell delivery purpose at NIH (Bethesda, MD, USA).
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