Cells were harvested, resuspended in serum-free medium, and seeded in the upper chamber of a Transwell insert containing a membrane filter (Corning, NY, USA). Medium containing 10% FBS was added to the lower chamber. The cells were incubated for 24 h. After treatment with paraformaldehyde, cells were stained with 0.1% crystal violet. Finally, cells were imaged and counted using an Olympus microscope (Tokyo, Japan).
Membrane filter
Manufactured by Corning
Sourced in United States
The Membrane Filter is a laboratory filtration device designed to capture and separate small particles or microorganisms from a liquid sample. It features a porous membrane that allows the passage of the liquid while retaining the desired material on the surface of the filter.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using membrane filter
1
Cell Migration Assay Protocol
2
Liposomal Formulation and Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Liposomes were prepared according to previous studies.57 (link) Liposomes were composed of PC (840053, Avanti, USA), PS, and cholesterol (CH) at a molar ratio of 2 : 1 : 1 (PSLs) or PC and CH at a 3 : 1 molar ratio (PCLs). Briefly, the phospholipids and CH were dissolved in ethyl alcohol. The solvent was evaporated and samples were dried under N2 gas for 3 h, resuspended in PBS, and sonicated for 3 min at 4 °C. Finally, the solution was passed through a membrane filter (0.45 μm, Corning Glassworks, Corning, NY) before use. Liposomal size and Zeta potential were determined by dynamic light scattering using Malvern Zetasizer (Malvern Instruments, Malvern, UK). The mean diameter and Zeta potential of PSLs and PCLs were ~231 nm, −40 mV and 208 nm, −5 mV, respectively. Liposomes (1 µM total lipids) were i.v. injected into mice immediately after ConA or LPS challenge.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!