Sodium deoxycholate

Melting points were determined on open glass capillaries using Gallenkamp apparatus and are uncorrected. Infrared spectra were measured on a Shimadzu FT-IR 8400s infrared spectrophotometer (cm⁻¹) using KBr disks, Faculty of Pharmacy, Cairo University, Cairo, Egypt. ¹H and ¹³C-NMR spectra were determined in DMSO-d₆ with Bruker Avance-400 spectrometer operating at 400 MHz and 100 MHz, respectively, at Applied Nucleic Acid Research Centre (ANARC), Faculty of Science, Zagazig University, Zagazig, Egypt. TLC was performed on 2.5 × 5 cm aluminum plates coated with silicagel 60F-254 (Merk, Germany) in an appropriate solvent. Span 60 was purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Sodium taurocholate (STC) and sodium deoxycholate (SDC) were purchased from BASF Co. (Florham Park, NJ, USA). 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N [methoxy(polyethylene glycol)-2000] (DSPE–mPEG-2000) was a generous gift from Lipoid GmbH (Ludwigshafen, Germany). Cholesterol was obtained from ITX Biomedicals (Santa Ana, CA, USA). Sodium hydroxide, potassium dihydrogen orthophosphate and absolute ethanol were purchased from El-Nasr Chemical Co., Cairo, Egypt. The Spectra/PoreVR dialysis membrane (12,000–14,000 molecular weight cut off) was purchased from Spectrum Laboratories Inc., Los Angeles, CA, USA. All chemicals and solvents were of HPLC grade and were used as received.

Sigma–Aldrich Chemical Co. (St. Louis, Missouri, USA) was the source of Resveratrol (RSV) and Span 60, while ITX Biomedicals (Santa Ana California, USA) was the source of Cholesterol. Sodium glycholate (SGC) and Sodium deoxycholate (SDC) were purchased from BASF Co. (Florham Park, New Jersey, USA). Absolute ethyl alcohol sodium hydroxide and potassium dihydrogen orthophosphate were acquired from El-Nasr Chemical Co., Cairo, Egypt. SERVA Electrophoresis GmbH., Heidelberg, Germany was the source of (12,000–14,000 molecular weight cut off) VISKING® dialysis membrane. All chemicals and solvents were of analytical grade and were used as received.