Buffer salts were purchased from Research Products International Corp. (RPI) and used without further purification. All crystallization materials were from Hampton Research. Metals were obtained as their chloride salts from J. T. Baker. L-Cysteine was purchased from Sigma Life Sciences. Fosfomycin disodium salt was from MP Biomedicals, LLC. BSH was synthesized as bacillithiol disulfide (BSSB) by the Vanderbilt Chemical Synthesis Core and reduced to BSH prior to use according to published procedures.19 (link) pET28 (FosBSa) was from Platinum PCR SuperMix, and custom primers were ordered from Invitrogen (Carlsbad, CA). Restriction enzymes were from New England Biolabs (Ipswich, MA). The pET20b(+) vector was from EMB Chemicals, Inc. (Gibbstown, NJ). XL1-Blue supercompetent cells and BL21-Gold (DE3) cells were from Agilent Technologies (Santa Clara, CA).
Fosfomycin disodium salt
Manufactured by MP Biomedicals
Fosfomycin disodium salt is a chemical compound used in laboratory settings. It serves as a broad-spectrum antibiotic that inhibits bacterial cell wall synthesis.
Automatically generated - may contain errors
Lab products found in correlation
Bl21 gold de3 cells, by Agilent Technologies (2 mentions)
Xl1 blue supercompetent cells, by Agilent Technologies (2 mentions)
L cysteine, by Merck Group (2 mentions)
Restriction enzyme, by New England Biolabs (2 mentions)
Custom primers, by Thermo Fisher Scientific (1 mentions)
Platinum pcr supermix, by Thermo Fisher Scientific (1 mentions)
2 protocols using fosfomycin disodium salt
1
Purification and Characterization of FosB Enzyme
2
Synthesis and Purification of Bacterial Cofactor
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Buffer salts were
purchased from
Research Products International Corp. (RPI) and used without further
purification. All crystallization materials were from Hampton Research.
Metals were obtained as their chloride salts from J. T. Baker.l -Cysteine was purchased from Sigma Life Sciences. Fosfomycin
disodium salt was from MP Biomedicals, LLC. BSH was synthesized as
bacillithiol disulfide (BSSB) by the Vanderbilt Chemical Synthesis
Core and reduced to BSH prior to use according to published procedures.19 (link) pET28 (FosBSa) was
from Platinum PCR SuperMix, and custom primers were ordered from Invitrogen
(Carlsbad, CA). Restriction enzymes were from New England Biolabs
(Ipswich, MA). The pET20b(+) vector was from EMB Chemicals, Inc. (Gibbstown,
NJ). XL1-Blue supercompetent cells and BL21-Gold (DE3) cells were
from Agilent Technologies (Santa Clara, CA).
purchased from
Research Products International Corp. (RPI) and used without further
purification. All crystallization materials were from Hampton Research.
Metals were obtained as their chloride salts from J. T. Baker.
disodium salt was from MP Biomedicals, LLC. BSH was synthesized as
bacillithiol disulfide (BSSB) by the Vanderbilt Chemical Synthesis
Core and reduced to BSH prior to use according to published procedures.19 (link) pET28 (FosBSa) was
from Platinum PCR SuperMix, and custom primers were ordered from Invitrogen
(Carlsbad, CA). Restriction enzymes were from New England Biolabs
(Ipswich, MA). The pET20b(+) vector was from EMB Chemicals, Inc. (Gibbstown,
NJ). XL1-Blue supercompetent cells and BL21-Gold (DE3) cells were
from Agilent Technologies (Santa Clara, CA).
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