HCC1428-LTED spheroids, were generated by seeding 2000 cells in 10% DCC medium, into ultralow attachment 96-well plates (Corning, UK) and centrifuged for 10 min at 900 rpm. After 3 days, spheres were treated with escalating doses of abiraterone and re-treated every 3–4 days. After 10 days of treatment images of spheres were taken using CeligoS (Nexcelome Bioscience, Lawrence, MA, USA) and viability was assessed using CellTitre-Glo® Luminescent 3D Cell Viability Assay (Promega, UK).
Celltitre glo luminescent 3d cell viability assay
Manufactured by Promega
Sourced in United Kingdom
The CellTiter-Glo® Luminescent 3D Cell Viability Assay is a quantitative method for determining the number of viable cells in a 3D culture. The assay measures the amount of ATP present, which is an indicator of metabolically active cells. The assay produces a luminescent signal that is proportional to the amount of ATP present, providing a simple method to quantify cell viability.
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2 protocols using celltitre glo luminescent 3d cell viability assay
1
Abiraterone Sensitivity in HCC1428-LTED Spheroids
2
Abiraterone Efficacy in HCC1428-LTED Spheroids
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