DAPT and lactacystine were purchased from Sigma–Aldrich Corp. (St. Louis, MO). Batimastat was from Calbiochem (Darmstadt, Germany). All other reagents were standard laboratory reagents.
Lactacystine
Manufactured by Merck Group
Sourced in Spain
Lactacystine is a chemical compound used in laboratory settings. It functions as an inhibitor of proteasome, a protein complex involved in the degradation of cellular proteins. Lactacystine is commonly utilized in research to study the role of the proteasome in various biological processes.
Automatically generated - may contain errors
Lab products found in correlation
Sodium succinate dibasic hexahydrate, by Merck Group (1 mentions)
Sodium lactate, by Merck Group (1 mentions)
Rapamycin, by Merck Group (1 mentions)
Ifn γ, by Thermo Fisher Scientific (1 mentions)
Insulin, by Merck Group (1 mentions)
Cycloheximide (chx), by Merck Group (1 mentions)
Dexamethasone (dex), by Merck Group (1 mentions)
Dimethyl fumarate, by Merck Group (1 mentions)
2 protocols using lactacystine
1
Chemical Reagents for Biological Assays
2
Modulating Tumor Cell Responses
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Inhibitors and activators were added 2 h prior to the treatment with the antitumoral agents (24 h). 3PO (Sigma, SML-1343; 10 µM), TOFA (Sigma, T6575; 1 µg/mL), C968 (Calbiochem, 352010; 40 nM, Madrid, Spain), sodium lactate (Sigma, L7022), sodium succinate dibasic hexahydrate (Sigma, S9637), di-methyl-fumarate (Sigma, 242926); lactacystine (Sigma, L6785; 10 µM), BBG (BioRad 161-0400; 10 µM, Alcobendas, Spain), LY294002 (Calbiochem, 440202; 10 µM), NFκB inhibitor SN50 (Calbiochem, 481480; 18 µM), actinomycin D (GE HealthCare Biosciences, 116128; 3 µg/mL), insulin (Sigma, 91077C; 100 nM), IFNγ (PeproTech, 300-02; 20 ng/mL), dexamethasone (Sigma, D4902; 1 µg/mL), rapamycin (Sigma, R0395, 10 nM), cycloheximide (Sigma, C6255; 10 µg/mL), rosiglitazone (Calbiochem, CAS155141-29-0; 1 µM), IFNα (Sigma, IF007, 20 ng/mL), MCC950 (Sigma, PZ0280; 300 nM), GW3965 (Merck, G6295, 1 μM), RAF inhibitor (Sigma, 475958; 10 μM), and PD98059 (Merck, HY-12028; 10 μM)
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