Anti phosphorylated p38 antibody

Manufactured by Cell Signaling Technology

The Anti-phosphorylated p38 antibody is a laboratory reagent used to detect and measure the levels of p38 mitogen-activated protein kinase (MAPK) that is phosphorylated. p38 MAPK is a key signaling protein involved in various cellular processes, and its phosphorylation status is an important indicator of cellular activation and response. This antibody specifically recognizes the phosphorylated form of p38 MAPK, allowing researchers to monitor the activation of this important signaling pathway.

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Close spelling variants of this product

Anti-dually phosphorylated p38 antibody Anti-phosphorylated p38 Phosphorylated p38 Anti-p38 antibody P38 antibody Anti-p38

2 protocols using anti phosphorylated p38 antibody

Immunohistochemical Analysis of Phosphorylated p38 in Breast Carcinoma

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A tissue array containing 50 cases total of breast carcinoma and matched metastatic carcinoma, and 4 cases of matched non-malignant breast tissues, were purchased from Us Biomax Inc. (Rockville, MD). Immunohistochemistry staining was performed according to the manufacturer’s instructions (Dako, Carpinteria, CA). In brief, the paraffin-embedded slides were deparaffinized with xylene and rehydrated through a graded alcohol series to water. Endogenous peroxidase activity was quenched with 3% hydrogen peroxide. The levels of pp38 were detected by using anti-phosphorylated p38 antibody (Cell Signaling Technology, Inc., Beverly, MA). Signal was detected using secondary biotinylated antibodies and streptavidin/horseradish peroxidase. Chromagen 3,3-diaminobenzidine/H₂O₂ (Dako) was used, and slides were counterstained with hematoxylin. All slides were observed under light microscopy, and images were captured with a SPOT RT camera (Diagnostic Instruments, Burlingame, CA).

He Z., He J., Liu Z., Xu J., Yi S.F., Liu H, & Yang J. (2014). MAPK11 in breast cancer cells enhances osteoclastogenesis and bone resorption. Biochimie, 0, 24-32.

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Signaling Pathway Regulation in Cell Lines

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The following reagents were purchased from the indicated manufacturers: rabbit polyclonal anti-human DR4 antibody, anti-human TRPV1 antibody, and anti-human HDAC1 antibody from Abcam (Cambridge, UK); mouse polyclonal anti-β-actin antibody and valproate from Sigma (St. Louis, MO); rabbit polyclonal anti-Histone H3 antibody, rabbit polyclonal anti-acetyl-histone H3 antibody and anti-acetyl-histone H4 antibody from Merck Millipore (Billerica, MA); rabbit polyclonal anti-Sp1 antibody, anti-Akt antibody, anti-phosphorylated Akt antibody, anti-PI3K antibody, anti-phosphorylated PI3K antibody, anti-STAT3 antibody, anti-phosphorylated STAT3 antibody, anti-p38 antibody, anti-phosphorylated p38 antibody, anti-p65 antibody, anti-phosphorylated p65 antibody, anti-ERK antibody, anti-phosphorylated ERK antibody, anti-JNK antibody, anti-phosphorylated JNK antibody, horseradish peroxidase (HRP)-anti-rabbit IgG and anti mouse IgG from Cell Signaling Technology (Beverly, MA); rh IGF-1 from R&D Systems (Minneapolis, MN); panobinostat from Cayman Chemical Company (Ann Arbor, MI); and 5-azacytidine and Akt inhibitor VIII from Calbiochem (Darmstadt, Germany). The human monoclonal anti-DR4 agonistic IgG antibody R1-B12 was kindly provided by Kyowa Hakko Kirin Co. Ltd. (Tokyo, Japan).

Amachi R., Hiasa M., Teramachi J., Harada T., Oda A., Nakamura S., Hanson D., Watanabe K., Fujii S., Miki H., Kagawa K., Iwasa M., Endo I., Kondo T., Yoshida S., Aihara K.I., Kurahashi K., Kuroda Y., Horikawa H., Tanaka E., Matsumoto T, & Abe M. (2016). A vicious cycle between acid sensing and survival signaling in myeloma cells: acid-induced epigenetic alteration. Oncotarget, 7(43), 70447-70461.

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