HeLa cells (American Type Culture Collection, Manassas, VA) stably modified to
express both firefly and Renilla luciferase were maintained at 37°C in high
glucose Dulbecco’s Modified Eagles Medium without phenol red (Invitrogen,
Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS, Invitrogen). 12
– 16 hours prior to transfection, cells were seeded in white 96-well plates at a
density of 15,000 cells per well. Cells were transfected with a 40 nM concentration of
anti-firefly luciferase siRNA (Dharmacon, Lafayette, CO) that had been formulated with
lipidoids into nanoparticles. Firefly luciferase silencing was assessed with a
Dual-Glo® Luciferase Assay kit (Promega, Madison, WI). Renilla
luciferase activity served as a control.
express both firefly and Renilla luciferase were maintained at 37°C in high
glucose Dulbecco’s Modified Eagles Medium without phenol red (Invitrogen,
Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS, Invitrogen). 12
– 16 hours prior to transfection, cells were seeded in white 96-well plates at a
density of 15,000 cells per well. Cells were transfected with a 40 nM concentration of
anti-firefly luciferase siRNA (Dharmacon, Lafayette, CO) that had been formulated with
lipidoids into nanoparticles. Firefly luciferase silencing was assessed with a
Dual-Glo® Luciferase Assay kit (Promega, Madison, WI). Renilla
luciferase activity served as a control.