Lunawave

Vfib occurrence was individually adjudicated by 2 independent cardiologists by reviewing ECG recordings during OCT/OFDI examinations identified by markings of each OCT/OFDI procedure. After adjudication of Vfib, the type of flushing agent, injection time length, injection flow rate, and injection volume of flushing agent were documented. The Vfib induction trigger such as R on T was also assessed and the length of time after the end of injection of flushing agent was measured. In all cases of Vfib, the time required Santa Clara, CA, USA) or Terumo OFDI (Lunawave®, Terumo Corporation, Tokyo, Japan). The technique of OCT/OFDI image acquisition has been described elsewhere. 1,3 OCT/OFDI pullbacks were performed automatically by the dedicated devices during injection of flushing agent, either contrast medium (iopamidol, Fuji Pharma Co., Ltd., Tokyo, Japan) or low-molecular-weight dextran with Ringer's lactate solution (LMWD) (Otsuka Pharmaceutical Factory, Inc., Tokushima, Japan), at a flow rate of 3-4 mL/s via the guiding catheter using an automated power injector pump (ACIST CVi, Eden Prairie, MN, USA). Pullback speed selection included 18 mm/s with the Abbott OCT system and 20 mm/s with the Terumo OFDI system. Invasive OCT/OFDI examinations were performed by 9 different

At 1 month after stent implantation, the animals were euthanized with a lethal dose of sodium pentobarbital (40 mg/kg, IV) after deep sedation with 5% inhaled sevoflurane; the heart was then gently removed (n=6 each) (Figure 1). Subsequently, saline and 10% neutral buffered formalin were immediately infused into the left coronary arteries via a constant perfusion pressure system (120 cm H2O). 16, 17 The harvested heart was then fixed with 10% neutral buffered formalin for 24 h. After the whole-heart sample preparation, we performed the OFDI imaging (LUNAWAVE; Terumo, Tokyo, Japan). We introduced an OFDI catheter (Fast View; Terumo, Tokyo, Japan) into the stented left coronary artery with the introduction of an intracoronary guide-wire, and then performed serial OFDI acquisition, with an automatic pullback rate of 40 mm/s.

OCT was performed by using either ILUMIEN OPTIS (Abbott Vascular, Santa Clara, CA, USA) or LUNAWAVE (Terumo, Tokyo, Japan). In cases with TIMI flow Grade 0 or 1, aspiration thrombectomy was performed prior to OCT imaging. The OCT catheter was advanced distally to the culprit lesion over a 0.014-inch conventional angioplasty lization, spontaneous coronary artery dissection, stent thrombosis, or coronary artery bypass graft failure were excluded from the study. Patients who had no stent implantation or inadequate OCT before and/or immediately after PCI were also excluded from the analysis.
This study was approved by the institutional review board of Wakayama Medical University Ethics Committee (Reference no. 1920). The requirement for written informed consent was waived because of the retrospective design of the study.