Log In Sign up
The largest database of trusted experimental protocols

Sb590885

Manufactured by R&D Systems
Visit Supplier

SB590885 is a small molecule inhibitor. It functions as a selective and potent inhibitor of a specific target enzyme.

Automatically generated - may contain errors

Lab products found in correlation

B27 supplement, by Thermo Fisher Scientific (2 mentions) Mitomycin c, by Thermo Fisher Scientific (1 mentions) Xav939, by Merck Group (1 mentions) Recombinant human lif, by Thermo Fisher Scientific (1 mentions) A419259, by Bio-Techne (1 mentions) Y 27632, by STEMCELL (1 mentions) N2 supplement, by Thermo Fisher Scientific (1 mentions) Gelatin, by Merck Group (1 mentions) L ascorbic acid, by Merck Group (1 mentions) Tak 632, by Selleck Chemicals (1 mentions) Encorafenib, by Selleck Chemicals (1 mentions) Vemurafenib, by Selleck Chemicals (1 mentions) Y27632, by Thermo Fisher Scientific (1 mentions) Activin a, by Thermo Fisher Scientific (1 mentions) Neurobasal, by Thermo Fisher Scientific (1 mentions) Rhlif, by Merck Group (1 mentions) Fibroblast growth factor 2 (fgf2), by Proteintech (1 mentions) 2 mercaptoethanol, by Thermo Fisher Scientific (1 mentions) Primocin, by Thermo Fisher Scientific (1 mentions) Bovine serum albumin (bsa), by Thermo Fisher Scientific (1 mentions)

4 protocols using sb590885

1

Differentiation of human embryonic stem cells into excitatory postsynaptic cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
hEPSC were cultured and maintained as described[5] with minor modifications. Briefly, the cells were cultured on mitomycin‐C (Thermo Fisher Scientific) inactivated STO feeder cells, which were seeded on 0.1% gelatin (Sigma Aldrich)‐coated wells at a density of 0.075 × 106 cells/cm2 at least 2 days prior to hEPSC seeding. The hEPSC medium: DMEM/F12, 1× L‐glutamine, 1× penicillin–streptomycin, 1× NEAA, 0.1 µm 2‐mercaptoethanol, 1× N2 supplement, 1× B27 supplement (Thermo Fisher Scientific), and 65 µg mL−1 L‐ascorbic acid (Sigma Aldrich) supplemented with 2.5 µm XAV939 (Sigma Aldrich), 0.15 µm A419259 (Tocris); 1.0 µm CHIR99021 (Stemgent), 0.25 µm SB590885 (R&D), and 10 ng mL−1 recombinant human LIF (PeproTech). 20% Knock‐out serum replacement (KOSR; Thermo Fisher Scientific) and 10 µm Y‐27632 (Stemcell Technologies) were supplemented to the medium on the day of hEPSC seeding and hEPSC were passaged every 3–4 days.
Chen A.C., Lee Y.L., Ruan H., Huang W., Fong S.W., Tian S., Lee K.C., Wu G.M., Tan Y., Wong T.C., Wu J., Zhang W., Cao D., Chow J.F., Liu P, & Yeung W.S. (2023). Expanded Potential Stem Cells from Human Embryos Have an Open Chromatin Configuration with Enhanced Trophoblast Differentiation Ability. Advanced Science, 10(11), 2204797.
+ Open protocol
+ Expand
2

Inhibitor Preparation for Cell Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols
TAK-632 (#S7291), Vemurafenib (PLX4032, #1267), and Encorafenib (LGX818, #S7108) were purchased from Selleck Chemicals LLC, Houston, TX, USA. Sorafenib tosylate (Axon 1397) was from Axon Medchem, Groningen, The Netherlands, and SB-590885 (2650/10) was purchased from R&D Systems, Minneapolis, MN, USA. Inhibitors were dissolved in DMSO. Stocks were kept at −80 °C and further diluted in DMSO as required.
Imoto H., Rauch N., Neve A.J., Khorsand F., Kreileder M., Alexopoulos L.G., Rauch J., Okada M., Kholodenko B.N, & Rukhlenko O.S. (2023). A Combination of Conformation-Specific RAF Inhibitors Overcome Drug Resistance Brought about by RAF Overexpression. Biomolecules, 13(8), 1212.
+ Open protocol
+ Expand
3

Resetting Human Embryonic Stem Cells to Naive State

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were reverted to the naive ground state in 5iLAF as described previously (Theunissen et al., 2014 (link)). At day 7, primed hESCs were dissociated into single cells with accutase and re-plated on MEFs in hESC media with Y27632 (Stemgent, 04–0012-10) at a density of 200k cells/well per 6-well plate. After one day, media was changed to 5iLAF media including a 50/50 mixture of DMEM/F12 (Gibco 11320–033) and Neurobasal (Gibco 21103–049), 1X N2 (Gibco 17502–048), 1X B27 (17504–044), 20 ng/mL rhLIF (Millipore LIF1005), 1 mM GlutaMAX (Gibco 35050–061), 1% NEAA (Gibco 11140–050), .1 mM 2-Mercaptoethanol (GIBCO, 21985–023), 1x Penicillin-Streptomycin (Gibco 15140–122), 50 ug/mL BSA (Gibco A10008–01), 1 mM PD0325901 (Stemgent 04–006-02), 1 mM IM-12 (BML-WN102–0005), .05 mM SB590885 (R&D 2650/10), 1 uM WH-4–023 (A Chemtek H620061), 10 uM Y-27632, 20 ng/mL Activin A (Peprotech AF-120–14E), 8 ng/mL FGF2 (Proteintech HZ1285), .50% KSR (Gibco 10828–028), and 1X primocin (Invitrogen (ant-pm-2). Media is changed daily and cells are passaged every 5 days at a ratio between 1:1 and 1:3 until robust colonies emerge.
Hancock G., Liu W., Peretz L., Chen D., Gell J., Collier A., Zamudio J., Plath K, & Clark A. (2021). Divergent roles for KLF4 and TFCP2L1 in Naive Ground State Pluripotency and Human Primordial Germ Cell Development. Stem cell research, 55, 102493.
+ Open protocol
+ Expand
4

SWR-PGCLC Expansion on MMC-treated m246 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
m246 feeder cells were treated with MMC (5 μg/ml) for 2 hours, washed with PBS, and then cultured with STO [DMEM, high glucose (Nacalai, #08458-45), 10% (v/v) FBS(Gibco, #10437028), 2 mM l-glutamine, and penicillin/streptomycin (50 U/ml)] medium for 5 hours. MMC-treated m246 cells were stored at −80°C. At 6 hours before sorting of SWR-PGCLCs, MMC-treated m246 feeder cells were seeded at 1 × 105 or 2 × 105 cells per well of a 24- or 12-well plate, respectively. SWR-PGCLCs at day 6 of induction were sorted and seeded at 5 × 103 or 1 × 104 cells per well of a 24- or 12-well plate, respectively, with expansion medium [advanced RPMI 1640, 10% (v/v) KSR, 2.5% (v/v) FBS, 1× NEAA, 1× GlutaMAX, and penicillin/streptomycin (50 U/ml)] supplemented with bFGF (20 ng/ml), 6 μM CHIR, 2.5 μM endo-IWR1, and 0.5 μM SB590885 (R&D Systems, #2650). The medium was then supplemented with 10 μM of Y-27632 for the first 48 hours of culture. The medium was changed every 2 days with the addition of 1 × 105 or 2 × 105 of MMC-treated m246 feeder cells to the well of the 24- or 12-well plate, respectively.
Hayashi M., Zywitza V., Naitou Y., Hamazaki N., Goeritz F., Hermes R., Holtze S., Lazzari G., Galli C., Stejskal J., Diecke S., Hildebrandt T.B, & Hayashi K. (2022). Robust induction of primordial germ cells of white rhinoceros on the brink of extinction. Science Advances, 8(49), eabp9683.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!