Jhos 4

The 293T (expressing SV40 T-antigen), CaOV3, and ES2 cell lines were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). JHOS-2, JHOS-4, and OVCAR3 cells were purchased from the RIKEN CELL BANK (Tsukuba, Japan). KURAMOCHI, OVSAHO, RMUGS, and TYK-nu cell lines were purchased from the Japanese Collection of Research Bioresources Cell Bank (JCRB, Osaka, Japan). 293T and CaOV3 cells were cultured in DMEM with 10% fetal bovine serum (FBS). JHOS-2 and JHOS-4 cells were cultured in DMEM/HamF12 medium with 10% FBS and 0.1 mM NEAA. ES2, KURAMOCHI, and OVSAHO cells were cultured in RPMI 1640 medium with 10% FBS, and OVCAR3 cells were maintained in RPMI 1640 medium with 20% FBS and 0.1% insulin. RMUGS cells were cultured in Ham’s F12 medium with 10% FBS. TYK-nu cells were cultured in EMEM with 10% FBS. All cell lines were certified by STR profiling cell line authentication (Supplementary Data 4). We routinely confirmed that these cell lines were negative for mycoplasma contamination using an e-Myco mycoplasma PCR detection kit (25235; iNtRON Biotechnology, Kirkland, WA, USA). For the serum starvation study, cells were treated with 0.2% FBS for 24 h, and for the hypoxia study, cells were treated with 2% oxygen for 24 h.

The OVTOKO, OVISE, OVMANA, RMG-I, OVSAHO, OVKATE, and OV1063 lines were purchased from the Japanese Collection of Research Bioresources (JCRB) Cell Bank (Osaka, Japan). The JHOC-7, JHOC-9, HTOA, JHOS-2, JHOS-3, and JHOS-4 cell lines were purchased from the RIKEN Cell Bank (Ibaraki, Japan). The TOV-21, ES-2, and SKOV3 cell lines were from the American Type Culture Collection (Manassas, VA). OVISE, OVTOKO, TOV-21G and ES2 were cultured in RPMI1640 medium containing 10% fetal bovine serum (FBS). OVMANA was cultured in RPMI medium containing 20% FBS, JHOC-7 in DMEM/F12 medium containing 10% FBS, JHOC-9 and RMG-I in DMEM/F12 medium containing 20% FBS, and SKOV3 in DMEM containing 10% FBS. The OVSAHO, OVKATE, OV1063, HTOA, JHOS-2, JHOS-3, and JHOS-4 lines were cultured in DMEM medium containing 10% FBS. The histological subtype of the SKOV3 cells was not unambiguously defined even after extensive analysis, although it was confidently identified as clear cell adenocarcinoma [22] (link). The immortalized epithelial cell line from an ovarian endometrial cyst was a generous gift from Dr. Satoru Kyo [23] (link).

JHOS2, JHOS3, and JHOS4 cell lines (RIKEN Cell Bank, Ibaraki, Japan) were cultured in Dulbecco’s modified Eagle’s medium (DMEM)/F12 medium, with 10% heat-inactivated fetal bovine serum (FBS) and 1% penicillin/streptomycin. KURAMOCHI, OVSAHO, and OVKATE cell lines (JRCB, Osaka, Japan) were cultured in RPMI medium with 10% heat-inactivated FBS and 1% penicillin/streptomycin. The TYK-nu cell line (JCRB) was cultured in DMEM with the addition of 10% heat-inactivated FBS and 1% penicillin/streptomycin. The OVCAR3 cell line (ATCC, Manassas, VA, USA) was cultured in RPMI medium with the addition of 20% heat-inactivated FBS and 1% penicillin/streptomycin. We cultured all cells in an incubator at 37 °C in humidified air with 5% CO₂.