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Recombinant mouse ifnα

Manufactured by R&D Systems
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Recombinant mouse IFNα is a laboratory reagent produced by R&D Systems. It is a type I interferon protein derived from a recombinant expression system. This product is intended for use in research applications.

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Lab products found in correlation

Gapdh, by Cell Signaling Technology (3 mentions) L nmma, by Merck Group (2 mentions) Total stat1, by Cell Signaling Technology (2 mentions) Pe anti mouse h 2kb h 2db, by BioLegend (2 mentions) Ptyr701 stat1, by Cell Signaling Technology (2 mentions) Total stat2, by Cell Signaling Technology (2 mentions) Total iκbα, by Cell Signaling Technology (2 mentions) Pe anti mouse ifnγr β chain, by BioLegend (2 mentions) Total p65 pser32 iκbα, by Cell Signaling Technology (2 mentions) Ptyr690 stat2, by Abcam (2 mentions) Lamin b, by Abcam (2 mentions) P p65 ser536, by Cell Signaling Technology (2 mentions) β actin, by Cell Signaling Technology (2 mentions) Tnf α, by R&D Systems (2 mentions) Ifn γ, by R&D Systems (2 mentions) Ifn β, by R&D Systems (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Actin, by Cell Signaling Technology (1 mentions) Tubulin, by Cell Signaling Technology (1 mentions) Mouse anti flag, by OriGene (1 mentions)

3 protocols using recombinant mouse ifnα

1

Antibody Reagents for Autophagy and Chikungunya Virus Research

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The rabbit anti LC3B (Cat # 2775), GAPDH (Cat #5174), Tubulin (Cat# 2148), ATG5 (Cat#12994), Actin (Cat # 8456) and anti-p62/SQSTM11(Cat# 5114, #7695) antibodies were purchased from Cell Signaling Technology (Danvers, MA 01923, USA). The mouse anti-FLAG (Cat# TA50011) and rabbit anti-human/mouse MSR1 (Cat# TA336699) antibodies were from Origene (Rockville, MD 20850, USA); the goat anti-mouse MSR1 (Cat# AF1797), mouse ant-human MSR1 (Cat# MAB2708), mouse anti-ATG12 (Cat# MAB6807) and recombinant mouse IFN-α (Cat #:12100-1) from R&D Systems (Minneapolis, MN 55413, USA). The mouse anti-CHIKV (Clone A54Q, Cat# MA5-18181) and Lipofectamine 2000 were obtained from ThermoFisher Scientific (Rockford, IL 61105, USA). The rat anti-CHIKV nsP1 (Cat# 111441) and nsP2 (Cat# 111442) were available from Antibody Research Corporation (St Peters, MO 63304, USA) and rabbit anti-CHIKV nsP1 (Cat# 11-13020) from ABGENEX (Bhubaneswar, Odisha 751024, India). DAPGreen (Cat# D676) was purchased from Dojindo Molecular Technologies, Inc. (Rockville, MD 20850).
Yang L., Geng T., Yang G., Ma J., Wang L., Ketkar H., Yang D., Lin T., Hwang J., Zhu S., Wang Y., Dai J., You F., Cheng G., Vella A.T., Flavell R.A., Fikrig E, & Wang P. (2020). Macrophage scavenger receptor 1 controls Chikungunya virus infection through autophagy in mice. Communications Biology, 3, 556.
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2

Flow Cytometry and Western Blot Analysis of Interferon Signaling

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The following antibodies were used in flow cytometry: PE anti-mouse H-2Kb/H-2Db, PE anti-mouse IFNγR β chain (Biolegend, San Diego, CA, USA), PE anti-mouse CD119 (IFNγ Receptor 1) (eBioscience, San Diego, CA, USA). Antibodies used in western blotting analysis were: iNOS, pTyr701-Stat1, total Stat1, total Stat2, pSer536-p65, total p65 pSer32-IκBα, total IκBα, β-actin, GAPDH (Cell Signaling Technology, Danvers, MA, USA); pTyr690-Stat2 (Abcam, Cambridge, MA, USA); LaminB (Epitomics, Burlingame, CA, USA). Recombinant mouse IFNα, IFNβ, IFNγ and TNFα were from R&D Systems (Minneapolis, MN, USA). L-NMMA was from Sigma-Aldrich (St. Louis, MO, USA).
Shou P., Chen Q., Jiang J., Xu C., Zhang J., Zheng C., Jiang M., Velletri T., Cao W., Huang Y., Yang Q., Han X., Zhang L., Wei L., Rabson A.B., Chin Y.E., Wang Y, & Shi Y. (2016). Type I interferons exert anti-tumor effect via reversing immunosuppression mediated by mesenchymal stromal cells. Oncogene, 35(46), 5953-5962.
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3

Flow Cytometry and Western Blotting of IFN Signaling

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The following antibodies were used in flow cytometry: PE anti-mouse H-2Kb/H-2Db, PE anti-mouse IFNγR β chain (Biolegend, San Diego, CA, USA) and PE anti-mouse CD119 (IFNγ Receptor 1) (eBioscience, San Diego, CA, USA). Antibodies used in western blotting analysis were iNOS, pTyr701-Stat1, total Stat1, total Stat2, pSer536-p65, total p65 pSer32-IκBα, total IκBα, β-actin, GAPDH (Cell Signaling Technology, Danvers, MA, USA); pTyr690-Stat2 (Abcam, Cambridge, MA, USA) and LaminB (Epitomics, Burlingame, CA, USA). Recombinant mouse IFNα, IFNβ, IFNγ and TNFα were from R&D Systems (Minneapolis, MN, USA). l-NMMA was from Sigma-Aldrich (St Louis, MO, USA).
Shou P., Chen Q., Jiang J., Xu C., Zhang J., Zheng C., Jiang M., Velletri T., Cao W., Huang Y., Yang Q., Han X., Zhang L., Wei L., Rabson A.B., Chin Y.E., Wang Y, & Shi Y. (2016). Type I interferons exert anti-tumor effect via reversing immunosuppression mediated by mesenchymal stromal cells. Oncogene, 35(46), 5953-5962.
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