The TBNC cell lines MDA-MB-157 (MDA-157), MDA-MB-231 (MDA-231), MDA-MB-468 (MDA-468), and HCC1806 were purchased (HTB-24, HTB-26, HTB-132, and CRL-2335, American Type Culture Collection, Manassas, VA, USA) within the previous 24 months and passaged < 15 times for all experiments (Table 1 ). Cells were tested biweekly for mycoplasma contamination (MycoAlert, Lonza, Basel, Switzerland). MDA-157, MDA-231, MDA-468, and MCF10A cells were grown in Dulbecco Modified Eagle Medium (DMEM High Glucose with GlutaMAX, Life Technologies, Carlsbad, CA, USA) and supplemented with 1% sodium pyruvate (Life Technologies) and 10% fetal bovine serum (FBS) (Premium Select, Atlantic Biologicals, Miami, FL, USA). HCC1806 cells were grown in RPMI 1640 medium (Life Technologies) supplemented with 10% FBS. Cells were maintained in a humidified 37°C incubator with 5% carbon dioxide.
Mda 468
Manufactured by Thermo Fisher Scientific
Sourced in United States
The MDA-468 is a laboratory equipment product manufactured by Thermo Fisher Scientific. It is designed to perform a specific function, but a detailed description cannot be provided while maintaining an unbiased and factual approach without extrapolation or interpretation. Additional information about the intended use or capabilities of this product is not available.
Automatically generated - may contain errors
Lab products found in correlation
Mda 231, by Thermo Fisher Scientific (4 mentions)
Mycoalert, by Lonza (4 mentions)
Mda mb 468, by American Type Culture Collection (3 mentions)
Htb 132, by American Type Culture Collection (3 mentions)
Htb 26, by American Type Culture Collection (3 mentions)
Htb 24, by American Type Culture Collection (3 mentions)
Mda 157, by Thermo Fisher Scientific (2 mentions)
Dmem high glucose with glutamax, by Thermo Fisher Scientific (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Mda 468, by American Type Culture Collection (2 mentions)
Crl 2335, by American Type Culture Collection (2 mentions)
Dmem high glucose glutamax, by Thermo Fisher Scientific (2 mentions)
Mda mb 231, by American Type Culture Collection (1 mentions)
Mda 157, by American Type Culture Collection (1 mentions)
Mcf 10a, by Thermo Fisher Scientific (1 mentions)
Mda mb231 mda 231, by American Type Culture Collection (1 mentions)
Hcc1806, by American Type Culture Collection (1 mentions)
Hcc1937, by American Type Culture Collection (1 mentions)
Crl 2336, by American Type Culture Collection (1 mentions)
Premium select, by R&D Systems (1 mentions)
4 protocols using mda 468
1
Cell Line Cultivation and Characterization
2
Breast Cancer Cell Lines Culturing Protocol
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MDA-MB-157 (MDA-157), MDA-MB-231(MDA-231) and MDA-MB-468 (MDA-468), MCF10A, and HCC1806 cells were purchased from the American Type Culture Collection (ATCC #’s HTB-24, HTB-26, HTB-132, CRL-10317, and CRL-2335, respectively) within the last 12 months and passaged < 15 times for all experiments. Cells were tested biweekly during experiments for mycoplasma contamination using the Lonza MycoAlert (Lonza #LT07-318). HCC1806 cells were grown in RPMI 1640 Medium (Life Technologies #11875093) and supplemented with 10% Fetal Bovine Serum (FBS, Atlantic Biologicals Premium Select). MDA-157, MDA-231, MDA-468, and MCF10A cells were grown in DMEM High Glucose + GlutaMAX (Life Technologies #10566016) and supplemented with 1% sodium pyruvate (Life Technologies #11360070) and 10% FBS. Cells were maintained in a humidified 37°C incubator with 5% carbon dioxide.
3
Breast Cancer Cell Line Cultivation
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The TNBC cell lines HCC1806, HCC1937, MDA-MB-157 (MDA-157), MDA-MB-231 (MDA-231), and MDA-MB-468 (MDA-468) were purchased from the American Type Culture Collection (Manassas, VA; CRL-2335, CRL-2336, HTB-24, HTB-26, and HTB-132). MX-1 was purchased from the NCI repository. All cell lines were purchased within the previous 24 months and passaged < 15 times for all experiments [Table 1] . Cells were tested biweekly for mycoplasma contamination (MycoAlert, Lonza, Basel, Switzerland). MX-1, MDA-157, MDA-231, and MDA-468 were grown in Dulbecco Modified Eagle Medium (DMEM High Glucose with GlutaMAX, Life Technologies, Carlsbad, CA) and supplemented with 1% sodium pyruvate (Life Technologies) and 10% fetal bovine serum (FBS) (Premium Select, Atlantic Biologicals, Miami, FL). HCC1937 and HCC1806 were grown in RPMI supplemented with 10% FBS. Cells were maintained in a humidified 37 °C incubator with 5% carbon dioxide.
4
Cell Line Cultivation and Validation
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MDA-MB-231(MDA-231), MDA-MB-468 (MDA-468), and HEK293T were purchased from the American Type Culture Collection (ATCC HTB-26, HTB-132, and CRL-3216, respectively; Manassas, VA, USA) within the last 24 months and passaged < 15 times for all experiments. Cells were tested biweekly during experiments for mycoplasma contamination using the Lonza MycoAlert® (Lonza #LT07-318). MDA-231 and MDA-468 cells were grown in DMEM High Glucose + GlutaMAX™ (Life Technologies, Carlsbad, CA, USA, #10566016) and supplemented with 1% sodium pyruvate (Life Technologies, #11360070) and 10% FBS (Premium Select, R&D systems, Minneapolis, MN, USA). HEK293T cells were grown in DMEM High Glucose + L-Glutamine (HyClone, Logan, UT, USA, # SH30022.01) and supplemented with 1% sodium pyruvate (Life Technologies #11360070) and 10% FBS. Cells were maintained in a humidified 37 °C incubator with 5% carbon dioxide.
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