Total RNA was extracted from mice liver tissues or cells using TRIzol reagent (Vazyme, R401-01, China). A total of 2 μg RNA was reversely transcribed using a HiScript® II Q RT SuperMix cDNA synthesis kit (Vazyme, R233-01, China). RT-qPCR was performed using a ChamQTM Universal SYBR® qPCR Master Mix (Vazyme, Q711-02, China). The relative expression levels of the genes were determined by SYBR-green-based RT-qPCR. The results were analyzed using the 2−ΔΔCT method and normalized toβ-actin values. Primer sequences are listed in Supplementary Table S1 .
Hiscript 2 q rt supermix cdna synthesis kit
Manufactured by Vazyme
Sourced in China
The HiScript® II Q RT SuperMix cDNA synthesis kit is a product designed for the reverse transcription of RNA into complementary DNA (cDNA). This kit includes a reverse transcriptase enzyme and other necessary components to facilitate the cDNA synthesis reaction.
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Lab products found in correlation
2 protocols using hiscript 2 q rt supermix cdna synthesis kit
1
Quantitative RNA Expression Analysis
2
Quantitative Real-Time PCR Analysis
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Total RNA from tissues or in vitro cultured cells was extracted with TRIzol reagent (Life Technologies) and cDNA was prepared with a Hiscript II Q RT SuperMix cDNA synthesis kit (Vazyme Biotech, China). qRT‒ PCR was performed to detect relative mRNA expression levels using SYBR Green master mix (Vazyme Biotech, China) on a QuantStudio 6 Real-Time PCR System (Life Technologies). The 2−ΔΔCT method was used to quantify fold induction. Each cDNA data point was normalized to GAPDH expression. The primer sequences are shown in Additional file 1 : Table S1.
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