HSCPs derived from BM were purchased from ATCC (Manassas, VA, USA) and cultured in StemMACS HSC growth media (Miltenyi Biotech, Auburn, CA, USA). HSCPs treated with SR1 (1 μM) were irradiated at 2Gy using a Gammacell 3000 Elan irradiator (137Cs γ-ray source; MDS Nordion, Ottawa, ON, Canada).
3000 elan
Manufactured by Nordion
Sourced in Canada
The 3000 Elan is a laboratory instrument designed for high-performance liquid chromatography (HPLC) analysis. It features a modular design and supports a range of HPLC applications. The core function of the 3000 Elan is to perform precise and reliable liquid chromatography separations and quantitative analysis.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using 3000 elan
1
Irradiated Hematopoietic Stem Cell Cultures
2
Generating Bone Marrow Chimeric Mice
3
Platelet Apheresis and Storage Characteristics
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Platelets were collected by standard apheresis procedures from 6 healthy donors (COBE Spectra®, n=4 and Trima Accel®, n=2, Terumo BCT, Lakewood, CO, USA). Acid citrate dextrose was used as anticoagulant [15] . The PC units contained 28828 x10 9 platelets on the day of donation (day 0). All PCs were irradiated with 25Gy on day 0 (Gammacell 3000 Elan, MDS Nordion, Ottawa, Canada). The PCs were stored in bags integrated to each collection kit on an agitator (Model LPR-3, Melco Engineering, Glendale, CA, USA) in an incubator (Helmer PC2200, Helmer, Noblesville, IN, USA) with a stable temperature of 22±2°C.
Samples were taken aseptically with a sampling bag (MacoPharma, Tourcoing, France) from each PC on days 1, 5, 7 and 12 (one PC on day 13 for flow cytometry measurements). One PC (prepared by COBE Spectra) had reduced swirling on day 12, thus as a comparison we performed flow cytometry measurements on samples from 5 additional PCs with decreased swirling (on day 15-22, collected by apheresis Trima Accel®, n=3 and Spectra Optia®, n=2, Terumo BCT). The eBDS system was used to check for bacterial growth (Haemonetics Corporation, Braintree, MA, USA).
Samples were taken aseptically with a sampling bag (MacoPharma, Tourcoing, France) from each PC on days 1, 5, 7 and 12 (one PC on day 13 for flow cytometry measurements). One PC (prepared by COBE Spectra) had reduced swirling on day 12, thus as a comparison we performed flow cytometry measurements on samples from 5 additional PCs with decreased swirling (on day 15-22, collected by apheresis Trima Accel®, n=3 and Spectra Optia®, n=2, Terumo BCT). The eBDS system was used to check for bacterial growth (Haemonetics Corporation, Braintree, MA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!