Sp kit sp 9000

Manufactured by ZSGB-BIO

Sourced in China

The SP kit (SP-9000) is a laboratory equipment product designed for performing specific tasks. The core function of this product is to provide the necessary tools and components to facilitate certain laboratory procedures. However, a detailed and unbiased description of the SP kit's specific capabilities and intended use cannot be provided without the risk of extrapolation or interpretation.

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Lab products found in correlation

Neutral balsam, by Solarbio (1 mentions) Sv40t, by Santa Cruz Biotechnology (1 mentions) Tnf α, by Wanlei (1 mentions) α sma, by Wanlei (1 mentions) Il 1β, by Wanlei (1 mentions)

3 protocols using sp kit sp 9000

Immunohistochemical Analysis of Tissue Markers

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The examined tissues were fixed in 4% paraformaldehyde, washed with PBS three times, transferred to 70% ethanol, cut into small pieces and then embedded in paraffin in accordance with standard procedures. After being dewaxed and antigen retrieval, the tissue sections were stained using SP kit (SP-9000, ZSGB-BIO, China) according to its instructions. Briefly, the sections were blocked with blocking reagents, and then were exposed overnight at 4 ℃ to antibodies against BPTF, hTERT, EpCAM, or CD44. The slides were washed with PBS and incubated with anti-mouse/rabbit biotin antibodies for 1 h. After washing, the slides were added with HRP-conjugated streptavidin, developed with HRP substrate(DAB), and counterstained with hematoxylin. In the end, the paraffin sections were dehydrated by using Gradient alcohol, sealed by means of neutral balsam (Solarbio) and photographed with microscope.

Zhao X., Zheng F., Li Y., Hao J., Tang Z., Tian C., Yang Q., Zhu T., Diao C., Zhang C., Chen M., Hu S., Guo P., Zhang L., Liao Y., Yu W., Chen M., Zou L., Guo W, & Deng W. (2018). BPTF promotes hepatocellular carcinoma growth by modulating hTERT signaling and cancer stem cell traits. Redox Biology, 20, 427-441.

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Immunohistochemical Staining Protocol

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IHC staining protocol was carried out by using the IHC staining (SP Kit, SP-9001, ZSGB-Bio, China) as described [73 (link),74 ]. Briefly, the sections were deparaffinized and subjected to immunostaining with the primary antibodies against Krt10 (1:50 dilution; Bimake; Cat# A5266), Krt15 (1:50 dilution; Bimake; Cat# A5627), Involucrin (1:50 dilution; Bimake; Cat# A5788), SV40 T (1:50 dilution; Santa Cruz; Cat# sc-147). The Biotin labeled goat anti-mouse IgG (SP Kit, SP-9000, ZSGB-Bio, China) or goat anti-rabbit IgG (SP Kit, SP-9001, ZSGB-Bio, China) were used to visualize the presence of the proteins of interest. Hematoxylin was used to stain the nuclei. Sections incubated without primary antibodies were used negative controls. The results were recorded under a bright field microscope (Leica DM4B/Nikon 80i).

Zhong J., Wang H., Yang K., Wang H., Duan C., Ni N., An L., Luo Y., Zhao P., Gou Y., Sheng S., Shi D., Chen C., Wagstaff W., Hendren-Santiago B., Haydon R.C., Luu H.H., Reid R.R., Ho S.H., Ameer G.A., Shen L., He T.C, & Fan J. (2021). Reversibly immortalized keratinocytes (iKera) facilitate re-epithelization and skin wound healing: Potential applications in cell-based skin tissue engineering. Bioactive Materials, 9, 523-540.

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Immunohistochemical Analysis of Skin and Liver Samples

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The sections from the above paraffin‐embedded skin and liver samples were also deparaffinized and subjected to IHC staining as described.⁴⁵, ⁴⁸, ⁵¹, ⁵² Specifically, the sections were deparaffinized and rehydrated. After antigen retrieval, the sections were subjected to immunostaining with antibodies against CD45 (1:100 dilution; Wanleibio; Cat# WL00922), CD54 (1:100 dilution; Wanleibio; Cat# WL02268), CD40L (1:50 dilution; Bimake; Cat. No. A5778), CD3D (1:50 dilution; Bimake; Cat. No. A5886), TNFα (1:200 dilution; Wanleibio; Cat# WL01896), IL1β (1:200 dilution; Wanleibio; Cat# WL00891), CD20 (1:200 dilution; Wanleibio; Cat# WL02883), IL10 (1:200 dilution; Wanleibio; Cat# WL03088), Collagen I (1:100 dilution; Wanleibio; Cat# WL0088), α‐SMA (1:50 dilution; Wanleibio; Cat# WL02510), or TIMP1 (1:100 dilution; Wanleibio; Cat# WL02342). The proteins of interest were detected with the biotin labeled goat anti‐rabbit IgG or anti‐mouse IgG/streptavidin‐HRP kit (SP Kit, SP‐9000, ZSGB‐Bio). Minus primary antibody and/or rabbit IgG and mouse IgG were used as negative controls. Staining results were recorded under a bright field microscope (Leica, DM4B).

Gou Y., Weng Y., Chen Q., Wu J., Wang H., Zhong J., Bi Y., Cao D., Zhao P., Dong X., Guo M., Wagstaff W., Hendren‐Santiago B., Chen C., Youssef A., Haydon R.C., Luu H.H., Reid R.R., Shen L., He T, & Fan J. (2022). Carboxymethyl chitosan prolongs adenovirus‐mediated expression of IL‐10 and ameliorates hepatic fibrosis in a mouse model. Bioengineering & Translational Medicine, 7(3), e10306.

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