After optimization, 100 μl of 5 μg/ml PCV3 VLPs in carbonate–bicarbonate buffer was coated onto a 96-well ELISA microplate (Corning, USA) at 4 °C overnight. The antigen-coated plate was washed three times with PBST and blocked with PBST containing 5% (w/v) skim milk at 37 °C for 3 h. After washing three times, 100 μl of diluted serum samples were added and incubated at 37 °C for 1 h. The plates were washed three times with PBST, followed by incubation for 1 h at room temperature with 100 μl diluted HRP-labeled goat anti-pig IgG (Solarbio, China, 1:5000). After being washed three times, the plates were incubated with 100 μl tetramethylbenzidine (TMB, Solarbio) in the dark for 15 min at room temperature, which was used as a chromogenic substrate. Reactions were stopped by adding 50 μl of ABTS stop solution (2 M HCl) and the absorbance (450 nm) was measured using an ELISA plate reader (PE, USA).
96 well elisa microplate
Manufactured by Corning
Sourced in United States
The 96-well ELISA microplate is a laboratory equipment designed for conducting enzyme-linked immunosorbent assay (ELISA) experiments. It consists of 96 individual wells arranged in a 8x12 grid format, providing a standardized platform for high-throughput sample processing and analysis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using 96 well elisa microplate
1
ELISA-based PCV3 Antibody Detection
2
FMDV Antibody Detection in Guinea Pigs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Serum samples collected weekly from the guinea pigs were evaluated by enzyme‐linked immunosorbent assay (ELISA). Initially, a 96‐well ELISA microplate (Corning Incorporated, Corning, ME) was coated with 100 µL of FMDV 146S antigen in 0.05 M of NaHCO3 coating buffer (pH 9.6) and incubated at 4°C overnight. After washing with phosphate buffered saline with Tween‐20 (PBST) three times, the microplate was blocked with 120 µL of PBST containing 1% bovine serum albumin for 1 hour at 37°C. Then, 100 µL of diluted serum (1:32) with PBST was incubated for 1 hour at 37°C, washed three times with PBST, and then patted dry. Afterward, 100 µL of HRP‐labeled rabbit anti‐guinea pig IgG (Sigma‐Aldrich, St. Louis, MO) was incubated as described above, followed by incubation with 100 µL of O‐phenylenediamine/hydrogen peroxide (H2O2) for 15 minutes at 37°C. The color reaction was stopped with 100 µL of 2 N H2SO4, and the optical density (OD) value was read using an ELISA reader (Bio‐Rad, CA, USA) at 490 nm.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!