The number of proliferating cells was counted using an automatic cell imaging counter (CYTORECON; GE Healthcare) or image cytometer (Cellomics CellInsight; Thermo Fisher Scientific).
Cytorecon
Manufactured by GE Healthcare
Sourced in Japan
CYTORECON is a laboratory device designed for the processing and analysis of cell samples. It performs automated cytoreduction, which is the process of reducing the number of cells in a sample to facilitate downstream analysis. The core function of CYTORECON is to prepare cell samples for further examination and testing.
Automatically generated - may contain errors
Lab products found in correlation
Cellomics cellinsight, by Thermo Fisher Scientific (1 mentions)
Nthy ori 3 1, by Sumitomo Pharma (1 mentions)
Rpmi 1640, by Merck Group (1 mentions)
Ccl22, by R&D Systems (1 mentions)
Dynabeads mouse t activator cd3 cd28, by Thermo Fisher Scientific (1 mentions)
Ril 2, by Thermo Fisher Scientific (1 mentions)
5 protocols using cytorecon
1
Automated Cell Counting Protocol
2
Thyroid Cancer Cell Lines: Cultivation
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Immortalized human thyroid epithelial cell line, Nthy-ori 3-1, was purchased from DS Pharma Biomedical (Osaka, Japan) [28 (link)]. Two DTC cell lines (TPC-1 and FTC-133) and two ATC cell lines (FRO and ACT-1) were used in the study. TPC-1, which originated from PTC, and FRO cells were a gift from Dr. Yamashita at Nagasaki University [40 (link)]. FTC-133, which originated from FTC, was kindly provided by Dr. Takeda at the Fourth Department of Internal Medicine, Shinshu University School of Medicine [41 (link)]. ACT-1 was established by Dr. Ohara at Tokushima University and was a kind gift [23 (link)]. All cell lines were maintained in RPMI 1640 (Sigma-Aldrich, Saint Louis, MO, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS) at 37°C under 5% CO2. Cell number was counted by using Cytorecon (GE healthcare, Tokyo, Japan).
3
Cell Viability Assay with TAM
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The cells were grown in six-well plates, and the number of viable cells following drug treatment was counted using CYTORECON (GE Healthcare Life Science, Tokyo, Japan). Cells (1 × 105 cells/well) were seeded in six-well plates and incubated for 24 h. Then, 2 mL of medium with 1–30 μM of TAM was added into each well. After each indicated period, the cell numbers were directly counted.
4
Cell Growth Kinetics Quantification
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Cells (2 × 105 cells/well) were seeded on a 6-well plate with 500 μL of RPMI 1640 containing 10% FBS. The number of cells was counted by using Cytorecon (GE healthcare, Tokyo, Japan) from 24 h until 96 h after seeding the cells.
5
Chemotaxis Assay of CD4+ T Cells
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Splenic CD4+ T cell were cultured for 5 days in RPMI 1640 containing 10% FBS with Dynabeads mouse T-activator CD3/CD28 (Invitrogen) at bead/cells ratio of 1:1 and 30 U/mL of rIL-2 (eBioscience). After serum starvation in RPMI 1640 medium for 3 h, splenic CD4+ T cells were seeded (1.0 × 106 cells in 400 μL) in Millicell Culture Plates Inserts (5.0-μm pore size, Merck Millipore). 600 μl of RPMI 1640 containing 0.1% BSA containing CCL22 (200 ng/mL; R&D Systems Inc.) was added to the lower chamber. The cells were cultured for 3 h at 37°C and then the numbers of migrated cells were counted by cell counter (CYTORECON, GE Healthcare UK).
CD4+ T cells purified from the spleen, cLNs, and salivary glands in SS model mice were cultured with CCL22 for 6 h. Then the mRNA of the T cells was purified for analysis of cytokine gene expression.
CD4+ T cells purified from the spleen, cLNs, and salivary glands in SS model mice were cultured with CCL22 for 6 h. Then the mRNA of the T cells was purified for analysis of cytokine gene expression.
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