Access ostase assay

Manufactured by Beckman Coulter

Sourced in China, United States

The Access Ostase assay is a laboratory test used to measure the levels of bone-specific alkaline phosphatase in the blood. This enzyme is produced by active bone-forming cells and provides information about bone formation activity.

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Lab products found in correlation

Elisa, by R&D Systems (1 mentions) Cobas e801 analyzer, by Roche (1 mentions) Enzyme linked immunosorbent assay kit, by Cusabio (1 mentions)

3 protocols using access ostase assay

Quantification of Biomarkers in Serum Samples

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Serum samples were collected on days 1 and 2; predose on days 8, 15, 22, 28; on day 64; and every 4 weeks thereafter and were analyzed for soluble MET, HGF, PlGF, VEGF-R2, c-Kit, sFlt-1, VEGF, sCTx, P1NP, and BALP. Pharmacodynamic effects were evaluated using time profiles with the mean ratio to baseline and standard error bars by treatment arms.
Levels of soluble MET, PlGF, VEGF, VEGF-R2, c-Kit, and sFlt-1 were quantified using multiplexed sandwich immunoassays with electrochemiluminescent detection (Meso Scale Discovery, Rockville, MD) following the manufacturer's instructions. Levels of HGF were analyzed using an analyte-specific enzyme-linked immunosorbent assay (ELISA; R&D Systems, Minneapolis, MN) following the manufacturer's instructions and were compared to a standard curve. Samples were prepared and analyzed as previously described [36 (link)]. Levels of sCTx were quantified using the Serum Crosslaps ELISA (IDS Nordic, Herlev, Denmark) following the manufacturer's instructions. Levels of P1NP were quantified by radioimmunoassay (RIA) using the UniQ PINP RIA kit following the manufacturer's instructions and were compared to a standard curve (Covance Laboratories). Levels of BALP were quantified using the Access Ostase assay, a one-step immunoenzymatic assay, following the manufacturer's instructions (Beckman Coulter, Indianapolis, IN).

Hong D.S., Rosen P., Lockhart A.C., Fu S., Janku F., Kurzrock R., Khan R., Amore B., Caudillo I., Deng H., Hwang Y.C., Loberg R., Ngarmchamnanrith G., Beaupre D.M, & Lee P. (2015). A first-in-human study of AMG 208, an oral MET inhibitor, in adult patients with advanced solid tumors. Oncotarget, 6(21), 18693-18706.

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Bone Turnover Biomarker Assessment

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At baseline and week 12, venous blood was collected in the ethylenediaminetetraacetic acid tube (BD Biosciences, San Jose, CA, USA) and serum-separated tube (BD Biosciences). The plasma was separated by centrifugation at 1500× g, 4 °C for 10 min, and serum was centrifuged at 1910× g, 4 °C, for 15 min. Serum osteocalcin (OC) was measured by electrochemiluminescence (Elecsys N-MID Osteocalcin ELISA Kit, Roche Diagnostics GmbH, Mannheim, Germany). Plasma C-telopeptide fragment (CTx), plasma N-telopeptide fragment (NTx), and serum bone-specific alkaline phosphatase (BALP) were assessed by the Elecsys β-CrossLaps/serum assay (Roche Diagnostics GmbH), enzyme-linked immunosorbent assay kit (Cusabio Biotech, Wuhan, China), and the Access Ostase assay (Beckman Coulter, Fullerton, CA, USA), respectively. Estradiol, FSH, and LH levels were determined by the Cobas e801 analyzer (Roche Diagnostics GmbH).

Kim J.E., Jeong H., Hur S., Lee J, & Kwon O. (2020). Efficacy and Safety of Kudzu Flower–Mandarin Peel on Hot Flashes and Bone Markers in Women during the Menopausal Transition: A Randomized Controlled Trial. Nutrients, 12(11), 3237.

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Osteoporosis Induction and Analysis

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The osteoporosis induction was done in the 50 dayold rats through the intramuscular administration of dexamethasone disodium phosphate (Decadron ® 4 mg/ml) at the dose level of 7 mg/Kg of body weight, once a week, during five weeks in all groups, except in the control group (Lucinda et al. 2010a (Lucinda et al. , b, 2013)) . The blood samples were centrifuged at 3000 rpm for 10 min and the serum was stored at -80 ºC during three months. Serum bone specific alkaline phosphatase (BAP) was measured using Access
Ostase assay (Beckman access, Beckman Coulter Inc. Fullerton, CA, USA) (Gao et al. 2011 ). The right tibias were removed and stored at -20 ºC for 30 days until the analysis, afterward they were thawed at room temperature for four hours. Finally, the tibias were placed centrally in their anatomical position on the scanner table.

Lucinda L.M.F., Aarestrup B.J.V., Reboredo M.M., Pains T.D.A., Chaves R.Z., Reis J.E.P., Louzada M.J.Q, & Guerra M.O. (2017). Evaluation of the anti-osteoporotic effect of Ginkgo biloba L. in Wistar rats with glucocorticoid-induced-osteoporosis by bone densitometry using dual-energy x-ray absorptiometry (DEXA) and mechanical testing. Anais da Academia Brasileira de Ciencias, 89(4).

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