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Pdh enzyme activity dipstick assay kit

Manufactured by Abcam
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The PDH Enzyme Activity Dipstick Assay Kit is a lab equipment product that allows for the quantitative measurement of pyruvate dehydrogenase (PDH) enzyme activity. The kit provides a simple and rapid method to determine PDH activity levels in biological samples.

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Lab products found in correlation

Molecular imager chemidoc xrs system, by Bio-Rad (3 mentions) Molecular imager chemidoc mp imaging system, by Bio-Rad (1 mentions)

Close spelling variants of this product

PDH activity kit Dipstick Assay kit Enzyme activity kit Activity assay kits Enzyme assay kits

4 protocols using pdh enzyme activity dipstick assay kit

1

Measuring PDH Complex Activity

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PDH complex activity in whole cells was measured using the Pyruvate dehydrogenase (PDH) Enzyme Activity Dipstick Assay Kit (ab109882, ABCAM, Cambridge, MA, USA) according to manufacturer’s instructions. Three biological replicates were used per measurement. Results are expressed as enzyme activity respect to control. The signal intensity was analyzed by a Molecular Imager ChemiDoc XRS + System (Bio-Rad Laboratories Inc., USA).
Álvarez-Córdoba M., Talaverón-Rey M., Villalón-García I., Povea-Cabello S., Suárez-Rivero J.M., Suárez-Carrillo A., Munuera-Cabeza M., Salas J.J, & Sánchez-Alcázar J.A. (2021). Down regulation of the expression of mitochondrial phosphopantetheinyl-proteins in pantothenate kinase-associated neurodegeneration: pathophysiological consequences and therapeutic perspectives. Orphanet Journal of Rare Diseases, 16, 201.
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2

Pyruvate Dehydrogenase Activity Quantification

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PDH complex activity in whole cells was measured using the Pyruvate dehydrogenase (PDH) Enzyme Activity Dipstick Assay Kit (ab109882, ABCAM, Cambridge, MA, USA) according to manufacturer’s instructions. Three biological replicates were used per measurement. Results are expressed as enzyme activity with respect to control. The signal intensity was analyzed by a Molecular Imager ChemiDoc™ MP Imaging System (Bio-Rad, Hercules, CA, USA).
Talaverón-Rey M., Álvarez-Córdoba M., Villalón-García I., Povea-Cabello S., Suárez-Rivero J.M., Gómez-Fernández D., Romero-González A., Suárez-Carrillo A., Munuera-Cabeza M., Cilleros-Holgado P., Reche-López D., Piñero-Pérez R, & Sánchez-Alcázar J.A. (2023). Alpha-lipoic acid supplementation corrects pathological alterations in cellular models of pantothenate kinase-associated neurodegeneration with residual PANK2 expression levels. Orphanet Journal of Rare Diseases, 18, 80.
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3

Measuring PDH Complex Activity

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PDH complex activity in whole cells was measured using the Pyruvate dehydrogenase (PDH) Enzyme Activity Dipstick Assay Kit (ab109882, ABCAM, Cambridge, MA, USA) according to manufacturer’s instructions. Three biological replicates were used per measurement. Results are expressed as enzyme activity respect to control. The signal intensity was analyzed by a Molecular Imager ChemiDoc XRS + System (Bio-Rad Laboratories Inc., USA).
Álvarez-Córdoba M., Reche-López D., Cilleros-Holgado P., Talaverón-Rey M., Villalón-García I., Povea-Cabello S., Suárez-Rivero J.M., Suárez-Carrillo A., Munuera-Cabeza M., Piñero-Pérez R, & Sánchez-Alcázar J.A. (2022). Therapeutic approach with commercial supplements for pantothenate kinase-associated neurodegeneration with residual PANK2 expression levels. Orphanet Journal of Rare Diseases, 17, 311.
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4

Measuring PDH Complex Activity

Check if the same lab product or an alternative is used in the 5 most similar protocols
PDH complex activity in whole cells was measured using the Pyruvate dehydrogenase (PDH) Enzyme Activity Dipstick Assay Kit (ab109882, ABCAM, Cambridge, MA, USA) according to manufacturer's instructions. Three biological replicates were used per measurement. Results are expressed as enzyme activity respect to control. The signal intensity was analyzed by a Molecular Imager ChemiDoc XRS+ System (Bio-Rad Laboratories Inc., USA).
Álvarez-Córdoba M., Talaverón-Rey M., Villalón-García I., Povea-Cabello S., Suárez-Rivero J.M., Suárez-Carrillo A., Munuera-Cabeza M., Salas J.J., & Sánchez‐Alcázar J.A. (2021). Down Regulation Of The Expression Of Mitochondrial Phosphopantetheinyl-Proteins In Pantothenate Kinase-Associated Neurodegeneration: Pathophysiological Consequences and Therapeutic Perspectives.
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