Chemidoc mp digital imaging system

Manufactured by Bio-Rad

Sourced in United Kingdom

The ChemiDoc MP digital imaging system is a high-performance imaging device designed for a variety of laboratory applications. It captures and analyzes images of chemiluminescent, fluorescent, and colorimetric samples, including Western blots, gels, and molecular arrays. The system features a highly sensitive CCD camera, advanced optics, and a user-friendly software interface to enable efficient image acquisition and data analysis.

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Lab products found in correlation

Image lab 5, by Bio-Rad (1 mentions) Trans blot turbo transfer system, by Bio-Rad (1 mentions) Semi dry turbo blotter system, by Bio-Rad (1 mentions) 0.2 μm nitrocellulose membrane, by Bio-Rad (1 mentions) Protein assay dye reagent, by Bio-Rad (1 mentions)

Close spelling variants of this product

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3 protocols using chemidoc mp digital imaging system

Western Blot Protein Detection

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Protein extracts (20 μg
proteins/lane) were separated by
SDS-PAGE using 10% Bis-Tris NuPAGE Novex Gel (Fisher Scientific, Loughborough,
U.K.) according to the protocol described in the Supporting Information. After running, gels were separated
and transferred to PVDF (poly(vinylidene difluoride)) membranes using
a Trans-Blot Turbo Transfer System (Bio-Rad Laboratories, U.K.) for
100 min at 125 V. Afterward, PVDF membranes were removed and blocked
in 5% milk in PBST (PBS, pH 7.2–7.5, 0.05% Tween-20) overnight
at 4 °C. Membranes were washed in PBST and then incubated overnight
at 4 °C with primary antibody mouse monoclonal anti-DNP (dinitro-phenyl-hydrazone)
(Sigma-Aldrich Co., St. Louis, Cat# A2831, RRID: AB_258033) at 1:1000
dilution. The following day, membranes were washed with PBST and incubated
at room temperature with corresponding horseradish goat antimouse
IgG/peroxidase (HRP)-conjugated secondary antibody (polyclonal goat
antimouse IgG; HRP, Millipore Cat# AP340P-50 ML, RRID: AB_1587164)
diluted at 1:1000. After 1 h of incubation, membranes were washed
in PBST and soaked with Western Enhanced Chemiluminescence substrates
supplied by the kit (Bio-Rad, Hertfordshire, U.K.). The emitted chemiluminescent
signals were detected by a ChemiDoc MP digital imaging system (Bio-Rad,
Hertfordshire, U.K.), and protein visualization was captured by Image
Lab 5.0 software (Bio-Rad).

Louati K., Kolsi F., Kallel R., Gdoura Y., Borni M., Hakim L.S., Zribi R., Choura S., Maalej A., Sayadi S., Chamkha M., Mnif B., Khemakhem Z., Boudawara T.S., Boudawara M.Z, & Safta F. (2023). Research of Pesticide Metabolites in Human Brain Tumor Tissues by Chemometrics-Based Gas Chromatography–Mass Spectrometry Analysis for a Hypothetical Correlation between Pesticide Exposure and Risk Factor of Central Nervous System Tumors. ACS Omega, 8(32), 29812-29835.

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SDS-PAGE and Western Blotting Protocol

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Total cell lysates were prepared in 1x reducing Laemmli sample buffer (2% SDS, 10 glycerol, 50 mM Tris pH 6.8, 2.5% β-mercaptoethanol, 0.002% bromophenol blue). Proteins were denatured by heating to 100 °C for 4 min prior to electrophoresis. Electrophoresis was performed using Bio-Rad 4–15% polyacrylamide mini-TGX gels in a Mini-Protean II system. Proteins were transferred to PVDF membrane using a semi-dry Turbo blotter system (Bio-Rad). Membranes were blocked for 1 h at room temperature in 5% low-fat milk powder in Tris buffered saline containing 0.2% Tween20 (1TBS.T) before incubation with primary antibody overnight at 4 °C. Following extensive washing in TBS.T, blots were incubated with HRP-conjugated secondary antibodies. Specific proteins were detected using Immobilon ECL reagent and a ChemiDoc-MP digital imaging system (Bio-Rad, Watford, U.K.).

Hawkins J.W., McNeill M.C., Ebrahimighaei R., Mellor H.H., Newby A.C, & Bond M. (2022). Cyclic-AMP Increases Nuclear Actin Monomer Which Promotes Proteasomal Degradation of RelA/p65 Leading to Anti-Inflammatory Effects. Cells, 11(9), 1414.

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Western Blot Analysis of Transfected Cells

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Transfected HTR8-SVneo cells were lysed in 1 x sodium dodecyl sulphate (SDS) gel loading buffer (50 mM Tris-HCl, pH 6.8, 2% SDS, 10% glycerol, 100 mM 2-mercaptoethanol) without dyes and immediately boiled for 5 min. Protein concentrations were determined by the Bradford Assay [46 (link)] using the Bio-Rad protein assay dye reagent (Cat. #500–0006; Bio-Rad Laboratories). Protein lysates (10 μg protein/lane) were separated by SDS-polyacrylamide gel electrophoresis (PAGE) and electroblotted to 0.2 μm nitrocellulose membranes (Cat #: 162–0097; Bio-Rad). After blocking the membranes with 5% skim milk in Tris-buffered saline Tween-20 (TBST; 20 mM Tris, 137 mM NaCl, and 0.1% Tween-20, pH 7.6) for 1 h, membranes were incubated with appropriate specific primary antibodies (Table 1). Immunoblots were then washed with TBST followed by incubation in horseradish peroxidase-conjugated secondary antiserum (Table 1) for 1 h. The immunoblots were again washed with TBST and protein-antisera complexes detected using the Pierce SuperSignal West Pico chemiluminescent substrate detection system (Cat. # 34080; ThermoFisher Scientific). Multiple exposures were acquired using a Bio-Rad ChemiDoc MP digital imaging system. Membranes were subsequently probed for Tubulin (TUBA) expression, which served as a loading control.

Kawamura E., Hamilton G.B., Miskiewicz E.I, & MacPhee D.J. (2018). Fermitin family homolog-2 (FERMT2) is highly expressed in human placental villi and modulates trophoblast invasion. BMC Developmental Biology, 18, 19.

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