The ARCHITECT HIV Ag/Ab Combo assay was performed according to the manufacturer’s instructions without modification. Plasma specimens were centrifuged for ten minutes at 8,000 rpm, transferred to polystyrene sample cups (Abbott Laboratories) in a total volume of 200μL, and then loaded onto the ARCHITECT i2000SR. The specimens were tested in singlicate due to volume limitations. The chemiluminescent signal, as measured by the instrument, is reported as a S/Co ratio of the relative light units (RLU) of the test sample to the RLU of the cutoff determined from an ARCHITECT calibrator. In addition to the kit controls, high and low external HIV-1 positive controls were included in each run. The controls were obtained from a proficiency testing panel derived from HIV-1-seropositive serum [22 (link)]. The high and low positive controls are consistent with long-term and recent infection, respectively, as measured by a previously characterized HIV incidence assay [23 (link)].
Polystyrene sample cups
Manufactured by Abbott
Polystyrene sample cups are disposable containers used for holding and transporting small liquid or solid samples. They are made of durable, lightweight polystyrene material. These cups are designed to provide a secure and hygienic way to handle and store samples in various laboratory and testing applications.
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2 protocols using polystyrene sample cups
1
ARCHITECT HIV Ag/Ab Combo Assay Protocol
2
Evaluation of ARCHITECT HIV Ag/Ab Assay
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The ARCHITECT HIV Ag/Ab Combo assay was performed according to the manufacturer's instructions without modification. Plasma specimens were centrifuged for ten minutes at 8,000 rpm, transferred to polystyrene sample cups (Abbott Laboratories) in a total volume of 200µL, and then loaded onto the ARCHITECT i2000SR. The specimens were tested in singlicate due to volume limitations. The chemiluminescent signal, as measured by the instrument, is reported as a S/Co ratio of the relative light units (RLU) of the test sample to the RLU of the cutoff determined from an ARCHITECT calibrator. In addition to the kit controls, high and low external HIV-1 positive controls were included in each run. The controls were obtained from a proficiency testing panel derived from HIV-1-seropositive serum [22] (link). The high and low positive controls are consistent with long-term and recent infection, respectively, as measured by a previously characterized HIV incidence assay [23] (link).
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