Log In Sign up
The largest database of trusted experimental protocols

Dapi mounting solution

Manufactured by Merck Group
Visit Supplier

DAPI mounting solution is a lab reagent used to mount and preserve fluorescently labeled samples for microscopic analysis. It provides a medium to affix the sample to a slide while maintaining the fluorescent signal.

Automatically generated - may contain errors

Lab products found in correlation

Goat anti human gpnmb, by R&D Systems (1 mentions) Nanozoomer 2ht, by Hamamatsu Photonics (1 mentions) Visiomorph, by Visiopharm (1 mentions)

Close spelling variants of this product

4′,6-diamidino-2-phenylindole (DAPI)-containing mounting solution Antifade-4 6-diamidino-2-phenylindole (DAPI) mounting solution 6-diamidino-2-phenylindole (DAPI) mounting solution DAPI-containing mounting solution Mounting solution DAPI solution

2 protocols using dapi mounting solution

1

Quantitative Analysis of gpNMB Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
gpNMB was stained using goat anti-human gpNMB (R&D Systems) primary antibody (1:500) at 4°C overnight, then with an anti-goat-Dylight 488 (Novus) secondary antibody (1:2500) at ambient temperature for 1 hr. Slides were counterstained with DAPI mounting solution (Sigma). The high-resolution images of tissue slices were acquired using a digital whole slide scanner (Nanozoomer 2-HT, Hamamatsu. Bridgewater, NJ) using a 20×/0.0.75 lens (Olympus, Center Valley, PA). The images were then quantified using the image analysis module of a digital pathology software Visiomorph (VisioPharm, Broomfield, CO).
Marquez-Nostra B.V., Lee S., Laforest R., Vitale L., Nie X., Hyrc K., Keler T., Hawthorne T., Hoog J., Li S., Dehdashti F., Ma C.X, & Lapi S.E. (2017). Preclinical PET imaging of glycoprotein non-metastatic melanoma B in triple negative breast cancer: feasibility of an antibody-based companion diagnostic agent. Oncotarget, 8(61), 104303-104314.
+ Open protocol
+ Expand
2

Apoptosis Detection in Embryos

Check if the same lab product or an alternative is used in the 5 most similar protocols
TUNEL staining was performed as described16 (link) with the following modifications
– after freezing embryos with liquid nitrogen, the embryos were fixed
with 4% PFA/PBS, pH 7.4 for 15 min at room temperature and then permeabilized by
incubation with 0.1% Triton X-100 in PBS for 10 min at room temperature. After
washing, TUNEL staining was performed according to the manufacturer’s
(Roche, Switzerland) instructions. Stained embryos were mounted using
Fluoroshield with DAPI mounting solution (Sigma-Aldrich) to visualize the
nuclei.
Dwivedi V.K., Pardo-Pastor C., Droste R., Kong J.N., Tucker N., Denning D.P., Rosenblatt J, & Horvitz H.R. (2021). Replication stress promotes cell elimination by extrusion. Nature, 593(7860), 591-596.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!