Agarose

Manufactured by HiMedia

Sourced in India

Agarose is a polysaccharide derived from red algae. It is a widely used material in laboratory settings for various applications, primarily in the field of molecular biology. The core function of agarose is to serve as a matrix for gel electrophoresis, a technique used to separate and analyze macromolecules such as DNA, RNA, and proteins based on their size and charge.

Automatically generated - may contain errors

Lab products found in correlation

Ethidium bromide, by HiMedia (3 mentions) Dimethyl sulfoxide (dmso), by HiMedia (3 mentions) Bromophenol blue, by HiMedia (3 mentions) Ethylene diamine tetraacetic acid (edta), by Merck Group (3 mentions) Catalase, by HiMedia (2 mentions) Ethanol, by HiMedia (2 mentions) Sodium chloride (nacl), by HiMedia (2 mentions) Ethylene diamine tetraacetic acid (edta), by HiMedia (2 mentions) Tris hcl, by HiMedia (2 mentions) Yeast extract, by HiMedia (2 mentions) 2 2 dipyridylketone, by Merck Group (2 mentions) Hs dna, by Merck Group (2 mentions) Sulphuric acid, by Merck Group (2 mentions) Sodium hydroxide (naoh), by HiMedia (2 mentions) T4 dna ligase, by Thermo Fisher Scientific (2 mentions) 2 deoxyadenosine, by Thermo Fisher Scientific (1 mentions) Acetonitrile, by Avantor (1 mentions) Uv spectrophotometry, by Hitachi (1 mentions) Sarkosyl, by Merck Group (1 mentions) Imidazole, by Merck Group (1 mentions)

23 protocols using agarose

Cell Line Authentication by STR Profiling

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Unless otherwise mentioned all reagents were of the highest purity available and obtained from Sigma Aldrich (now Merck, St. Louis, MO, USA); Agarose, ethidium bromide, catalase, ethanol, DMSO, NaCl, EDTA, Tris-Cl were obtained from HiMedia (Mumbai, India); DMEDA from Tokyo Chemical Industry (Tokyo, Japan); acetonitrile from JT Baker (Center Valley, PA, USA); glutathione from Sisco Research Lab. Pvt Ltd. (Mumbai, India); 2′-deoxyadenosine from Alfa Aesar (Haverhill, MA, USA); ethylamine and propylamine from Spectrochem Pvt Ltd. (Mumbai, India).
Cell lines authentication by STR profiling was outsourced to Lifecode Technologies (New Delhi, India).

Siddiqui S., Ahmed N., Goswami M., Chakrabarty A, & Chowdhury G. (2021). DNA damage by Withanone as a potential cause of liver toxicity observed for herbal products of Withania somnifera (Ashwagandha). Current Research in Toxicology, 2, 72-81.

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Genomic DNA Extraction from Blood

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Genomic DNA was extracted from the frozen blood by phenol-chloroform method [10 ]. For DNA extraction, 500 μL of blood was used and the isolated DNA dissolved in TE was stored at −20°C. The quality of the DNA was checked in 0.7 percent agarose (Hi-Media, Mumbai) gel electrophoresis and quantified using UV spectrophotometry (Hitachi, Japan).

Lourdhu Mary A., Nithya K., Isabel W, & Angeline T. (2014). Prevalence of Catalase (-21 A/T) Gene Variant in South Indian (Tamil) Population. BioMed Research International, 2014, 894237.

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Protein Purification and Biophysical Characterization

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Ni-NTA Agarose was purchased from Qiagen (USA). Alexa Fluor, Thioflavin-T (ThT), Tris-base, ampicillin, chloramphenicol, sodium dodecyl sulfate (SDS), sarkosyl, dithiothreitol (DTT), phenylmethanesulfonyl fluoride (PMSF), isopropyl β-D-1-thiogalactopyranoside (IPTG), and imidazole were procured from Sigma (USA). Guanidine hydrochloride (GdnHCl), TCEP ammonium persulphate (APS) were purchased from SRL (India). Bradford's protein concentration estimation reagent was from Bio-Rad (USA). EDTA-free protease inhibitor cocktail was purchased from Roche Diagnostics (Switzerland). Agarose, Coomassie brilliant blue R-250, tetramethylethylenediamine (TEMED), sodium phosphate dibasic and sodium phosphate monobasic were purchased from HiMedia Lab (India). Urea was purchased from Affymetrix (USA). Restriction enzymes were procured from NEB BioLabs (USA). Dimebon was purchased from Sigma (USA). Diphenhydramine hydrochloride (DPH) was procured from TCI chemicals (Canada). Alexafluor 488 C5 malemide (catalogue number: A10254) was purchased from Thermofischer scientific, USA.

Girdhar A., Bharathi V., Tiwari V.R., Abhishek S., Deeksha W., Mahawar U.S., Raju G., Singh S.K., Prabusankar G., Rajakumara E, & Patel B.K. (2020). Computational insights into mechanism of AIM4-mediated inhibition of aggregation of TDP-43 protein implicated in ALS and evidence for in vitro inhibition of liquid-liquid phase separation (LLPS) of TDP-43^2C-A315T by AIM4. International journal of biological macromolecules, 147.

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SDS-PAGE and Silver Staining Protocol

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Tris-HCl, bovine serum albumin (BSA) and agarose were purchased from Hi-Media, Mumbai, India. Sodium dodecyl sulphate (SDS), ammonium per sulphate (APS), N,N,N′,N′-tetramethylethanediamine (TEMED), bromophenol blue, glycerol, acrylamide/bis, silver nitrate, sodium thiosulphate, sodium carbonate, formaldehyde (37 mL/100 mL) and Na-EDTA were purchased from SD Fine Chemicals Pvt. Ltd., Mumbai, India. Immobilized non-linear pH gradient (IPG strips) of pH 3–10 and 4–7, Bradford reagent, rehydration buffer, equilibration buffer I and II for 2D-GE were purchased from Bio-Rad, Hercules, USA. All chemicals used were of molecular biology grade. All reagents were prepared fresh during each assay.

Amane D, & Ananthanarayan L. (2019). Application of two-dimensional gel electrophoresis technique for protein profiling of Indian black gram varieties and detection of adulteration in black gram-based food products using comparative proteomics. Food Chemistry: X, 3, 100051.

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Synthetic Lipid Nanoparticle Preparation

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Fmoc-amino acids, p-toluene sulfonic acid, dicyclohexylcarbodiimide (DCC), 4-dimethylaminopyridine, and 1-bromo tetradecane were delivered from Sigma Aldrich. The organic solvents and chemicals, including ethanolamine, potassium carbonate, piperidine, dimethylformamide (DMF), methylene dichloride (DCM), and ethyl acetate, were purchased from Spectrochem Pvt. Ltd., and Finar Limited. Molecular mass analyses were performed by High-Resolution Mass Spectrometry (HRMS) using Agilent Q-TOF 6230, and the purity of final lipids was characterized by HPLC (Shimadzu LC Solution). ¹H-NMR (400 MHz) and ¹³C-NMR (101 MHz) spectra were recorded on Bruker AVANCE spectrometer, with TMS as an internal standard. DOPE (1,2-dioleoyl-sn-glycerol-3-phosphoethanolamine) was purchased from Sigma Aldrich. Agarose and 6× loading dye were purchased from Hi-media, India. All chemicals for biological applications were procured from Merck, Sigma-Aldrich, Thermo Fischer Scientific, and Invitrogen.

Manturthi S., Narayan K.P, & Patri S.V. (2022). Dicationic amphiphiles bearing an amino acid head group with a long-chain hydrophobic tail for in vitro gene delivery applications. RSC Advances, 12(51), 33264-33275.

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Comprehensive Biochemical Techniques in Research

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2,3,5-triphenyltetrazolium Chloride (TTC) (Himedia, India), Dimethylsulfoxide (DMSO), sorbitol (0.8 M) (Srichem, India), agarose (Himedia, India), Ethidium bromide (Himedia, India), Bromophenol blue (Himedia, India), xylene cyanol FF (Himedia, India), glycerol (Himedia, India), Tris, base (Himedia, India), Tris-Hcl (Himedia, India), Ethylenediamine teraacetic acid (EDTA) dihydrate (Fischer Scientific), Mueller-Hinton agar (Himedia, India), Mueller-Hinton broth (Himedia, India), Genomic DNA extraction kit (GeneiTM, India), DNA ladder (1 Kb, Sigma, India), Whatman No. 1 filter paper (Whatman GE Healthcare Company). All the other chemicals of analytical grade were purchased from Sigma-Aldrich India Limited, Hi Media, India and Merck, India.

Afagnigni A.D., Nyegue M.A., Janmeda P., Sharma V.K., & Etoa F. (2021). The Ethanolic Leaf Extracts of Dissotis multiflora (Sm) Triana and Paullinia pinnata Linn Exert Inhibitory Effect on Escherichia coli Through Membrane Permeabilization, Loss of Intracellular Material, and DNA Fragmentation. Journal of Drug Delivery and Therapeutics, 11(2-S), 4-13.

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Plant DNA Isolation and PCR Analysis

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Plant DNA isolation kit was purchased from Favorogen, polymerase chain reactions (PCR) master mix (2X) from Genetix Biotech Asia Pvt. Ltd., New Delhi, India. Agarose, 6X gel loading dye and 100 bp and 1 Kb DNA ladder were procured from Himedia Laboratories PVT, LTD, Mumbai, India, Primers mat k, rbcl, and ITS-4 and 5 was synthesized by Eurofins Genomics India Pvt. Ltd, Bangalore, Karnataka, India. All other chemicals and solvents were of analytical grade and obtained from Himedia, Mumbai, India.

Marimuthu S., Prasad G.P., Pratap G., Prasad S.B., Rao G.S., Mangal A.K., & Srikanth N. (2020). Molecular identification and next-generation sequence analysis of interspecies genetic variations among three varieties of Datura. Pharmacognosy Research, 12(2), 158-158.

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Comprehensive Molecular Reagents Protocol

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All osmolytes (sucrose, mannose, sorbitol, mannitol, trimethylamine N-oxide, trehalose), amino acids, lithium acetate, Tris-HCl, EDTA and salmon-sperm DNA (ssDNA) were purchased from Sigma-Aldrich (St. Louis, MO). Yeast extract, dextrose, peptone, agarose and polyethylene glycol (PEG) were purchased from Hi-media Laboratories (Mumbai, India).

Bandyopadhyay A., Bose I, & Chattopadhyay K. (2019). Osmolytes ameliorate the effects of stress in the absence of the heat shock protein Hsp104 in Saccharomyces cerevisiae. PLoS ONE, 14(9), e0222723.

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Synthesis and Antimicrobial Evaluation

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All analytical grade chemicals and solvents were used as commercially received. HAuCl₄^.3H₂O was purchased from S.D. Fine-Chem Ltd. (India.). 2,2-Dipyridylketone, 2-benzoyl pyridine, benzaldehyde, 4-methyl benzaldehyde, 4-bromo benzaldehyde, 4-chloro benzaldehyde, 4-methoxy benzaldehyde, HS-DNA and EDTA were purchased from Sigma Aldrich Chemical Co. (India). Agarose, Luria Broth (LB), ethidium bromide (EtBr), Tris–acetyl–EDTA (TAE) and bromophenol blue were purchased from Himedia (India). Culture for antibacterial activity Bacillus subtilis (B. subtilis-7193), Staphylococcus aureus (S. aureus-3160), Pseudomonas aeruginosa (P. aeruginosa-1688), Escherichia coli (E. coli-433) and Serratia marcescens (S. marcescens-7103) were purchased from the Institute of Microbial Technology (Chandigarh, India). S. Pombe Var. Paul Linder 3360 was obtained from IMTECH, Chandigarh.

Kanthecha D.A., Bhatt B.S, & Patel M.N. (2019). Synthesis, characterization and biological activities of imidazo[1,2-a]pyridine based gold(III) metal complexes. Heliyon, 5(6), e01968.

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Iron and Nucleic Acid Manipulation

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Iron chloride hexahydrate (FeCl_3.6H₂O), iron chloride tetrahydrate (FeCl_2.4H₂O), sodium hydroxide (NaOH), sodium chloride (NaCl), ethylenediaminetetraacetic acid (EDTA), tris buffer, glycine, agarose, ethidium bromide (EtBr), triacetate EDTA buffer (TAE), DNA ladder, and loading dye were purchased from Himedia laboratories Pvt Ltd. Additionally, absolute ethanol, potassium bromide (KBr) were purchased from Sigma Aldrich Pvt ltd. RNA extraction kit and RT-PCR kit were purchased from MB615 Himedia Pvt Ltd, and MBPCR243 Himedia Pvt Ltd, respectively.

Gambhir R.P., Parthasarathy A.K., Sharma S., Kale S., Magdum V.V, & Tiwari A.P. (2022). pH-responsive glycine functionalized magnetic iron oxide nanoparticles for SARS-CoV-2 RNA extraction from clinical sample. Journal of Materials Science, 57(28), 13620-13631.

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