Invertoskop microscope

Manufactured by Zeiss

The Invertoskop is a microscope designed by Zeiss for professional laboratory use. It features a reversible optical path, allowing the observation of specimens in both the upright and inverted positions. The microscope is equipped with high-quality optics and a robust construction, making it suitable for a variety of applications in the scientific and research fields.

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Close spelling variants of this product

Invertoskop 40C Microscope Invertoskop

3 protocols using invertoskop microscope

Astrocyte Proliferation Kinetics

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Cortical and cerebellar astrocytes were seeded in 12-well plates precoated with poly-L-lysine at a density of 5 × 10⁴ cells/well in a culture medium. The day for seeding the cells was set as day 0, and cortical and cerebellar astrocytes were harvested at day 3 to day 6 using trypsin-EDTA and were counted with a hemocytometer. Cell counting was performed by a different researcher who was blinded to the groups using a Zeiss Invertoskop microscope.

Sun Y., Winters A., Wang L., Chaudhari K., Berry R., Tang C., Liu R, & Yang S. (2023). Metabolic Heterogeneity of Cerebral Cortical and Cerebellar Astrocytes. Life, 13(1), 184.

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Astrocyte Glucose Metabolism Study

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Astrocyte were seeded into 12-well (40,000 per well) or 24-well (25,000 per well) culture plates in DMEM with pyruvate and 10% FBS. Cells were cultured for two days, and then medium was replaced with new normal glucose (5.5 mM) or high glucose (25 mM) DMEM (with pyruvate and 10% FBS). Plates were incubated in a humidified incubator at 37 °C and 5% CO₂. Cells were harvested on each indicated day using 0.25% trypsin-EDTA (Invitrogen) and counted using an inverted phase contrast Zeiss Invertoskop microscope.

Li W., Roy Choudhury G., Winters A., Prah J., Lin W., Liu R, & Yang S.H. (2018). Hyperglycemia Alters Astrocyte Metabolism and Inhibits Astrocyte Proliferation. Aging and Disease, 9(4), 674-684.

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Astrocyte Proliferation and Cell Cycle Analysis

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Astrocytes were seeded at a density of 40,000 cells/well in 12-well plates or 25,000 cells per well in 24-well plates with different culture medium. At the indicated days after seeding, cells were harvested using trypsin-EDTA and counted using a hemocytometer. Four wells were assigned to each group and cell counting was conducted by a researcher who was blinded to the group assignment using an inverted phase contrast Zeiss Invertoskop microscope.
For cell cycle analysis astrocytes were seeded at a density of 50,000 cells/well in 12-well plates in various culture media. On day 7 after culture, cells were harvested using trypsin-EDTA and washed with buffer (PBS) twice to remove trypsin. Cells were fixed in ice-cold 70% ethanol for 24 hours at 4°C. Then, cells were incubated with propidium iodide (PI) (40 μg/ml) and RNase (10 μg/ml) for 30 minutes at 37°C. The stained cells were analyzed using a Beckman Coulter FC500 Flow Cytometry Analyzer for quantification of cell cycle distribution (G1, S or G2/M).

Prah J., Winters A., Chaudhari K., Hersh J., Liu R, & Yang S.H. (2019). A Novel Serum Free Primary Astrocyte Culture Method that Mimic Quiescent Astrocyte Phenotype. Journal of neuroscience methods, 320, 50-63.

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