Gitr pe

Manufactured by Miltenyi Biotec

The GITR-PE is a laboratory equipment product manufactured by Miltenyi Biotec. It is used for the detection and analysis of GITR (Glucocorticoid-Induced TNFR-Related Protein) expression in various cell types. The GITR-PE utilizes a phycoerythrin (PE) conjugated antibody to identify and quantify GITR-positive cells through flow cytometry or other analytical techniques.

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Lab products found in correlation

Lsrfortessa flow cytometer, by BD (1 mentions) Prism 6, by GraphPad (1 mentions) Cd25 pe, by BD (1 mentions) Diva software, by BD (1 mentions) Cd45ra apc, by BD (1 mentions) Cd3 apca750, by Beckman Coulter (1 mentions) Cd4 ecd, by Beckman Coulter (1 mentions) Cd16 fitc, by Beckman Coulter (1 mentions) Cd19 ecd, by Beckman Coulter (1 mentions) Cd127 fitc, by Thermo Fisher Scientific (1 mentions) Cd8 apc a700, by Beckman Coulter (1 mentions) Dynabeads mouse t activator cd3 cd28, by Thermo Fisher Scientific (1 mentions) Cd8α fitc, by Miltenyi Biotec (1 mentions) Tnfr2 apc, by Miltenyi Biotec (1 mentions) Foxp3 staining buffer set kit, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

PE-anti-GITR (2 citations)

2 protocols using gitr pe

Profiling T-cell activation and pSTAT5 signaling

Cited 1 time

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Flow cytometry and basal pStat5 experiments were performed according to previously published methods [17 (link), 41 (link)]. Monoclonal antibodies (mAbs) used were CD3-APCa750, CD4-ECD, CD8-APCa700, CD16-FITC, CD19-ECD, CD45RO-FITC, CD56-PCy7 and CD152-PE from Beckman Coulter; CD25-PE, CD45RA-APC and CD45RA-PCy7 from BD Biosciences; CD127-FITC from eBioscience; CD25-APC and LAP-PE from R&D Systems and GITR-PE from Miltenyi.
For pSTAT5 activation experiments, peripheral blood mononuclear cells (PBMCs) were cultured (500,000/well) for 30 min at 37° C and IL-2 was added for 15 min. Cells were then fixed with 1.6% paraformaldehyde, permeabilized with 100% methanol, stained for pSTAT5 and appropriate surface markers. Events were collected using a LSRFortessa flow cytometer (BD Biosciences) and data were analyzed using Diva software (BD Biosciences). To calculate EC50, after subtracting the value from the media control, binding data for each sample was normalized to 100% based on the maximal response and non-linear regression of these data was performed assuming a variable slope using Graph-Pad Prism 6.0.

Rosenzwajg M., Churlaud G., Mallone R., Six A., Derian N., Chaara W., Lorenzon R., Long A., Buckner J., Afonso G., Pham H.P., Hartemann A., Yu A., Pugliese A., Malek T.R, & Klatzmann D. (2015). Low-dose interleukin-2 fosters a dose-dependent regulatory T cell tuned milieu in T1D patients. Journal of autoimmunity, 58, 48-58.

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Immunosuppressive Activity of MSCs

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3 × 10⁴ WT or TNFR2 KO-MSCs were co-cultured in 12-well plates with 1.5 × 10⁵ (1/5 ratio) of mice WT-CD3⁺CD25⁻T cells in a total volume of 2 ml. We used this intermediate fixed ratio since we observed 50% immunosuppressive activity by MSCs. WT-CD3⁺CD25⁻T cells were stimulated by Dynabeads Mouse T-Activator CD3/CD28 (Gibco). 2 × 10⁵ freshly isolated WT-CD3⁺CD25⁻T cells were used as control T cells alone. After 3 days, WT-CD3⁺CD25⁻T cells were harvested and immunostained with CD4-VIOBLUE and CD8α-PeCy5 or CD8α-FITC, ICOS-PeCy7, GITR-PE, CD25-PeCy7 or PE, and TNFR2-APC (Miltenyi). Intracellular Foxp3 staining was performed according to the manufacturer’s instructions, using the Foxp3 staining buffer set kit (eBioscience).

Beldi G., Khosravi M., Abdelgawad M.E., Salomon B.L., Uzan G., Haouas H, & Naserian S. (2020). TNFα/TNFR2 signaling pathway: an active immune checkpoint for mesenchymal stem cell immunoregulatory function. Stem Cell Research & Therapy, 11, 281.

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