Quanta 200 feg sem

Manufactured by Thermo Fisher Scientific

Sourced in United States, Australia, Netherlands

The Quanta 200 FEG SEM is a field emission scanning electron microscope (FEG SEM) designed for high-resolution imaging and analysis of a wide range of materials. It features a Schottky field emission gun, which provides high brightness and small probe size for high-resolution imaging. The Quanta 200 FEG SEM is capable of operating in high and low vacuum modes, as well as in environmental mode, allowing for the analysis of a variety of sample types.

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Lab products found in correlation

Autosamdri 815b, by Tousimis (3 mentions) Dxr raman microscope, by Thermo Fisher Scientific (2 mentions) Escalab 250xi, by Thermo Fisher Scientific (2 mentions) Jes fa200, by JEOL (1 mentions) Ultraflextreme, by Bruker (1 mentions) 3view system, by Ametek (1 mentions) Digital micrograph, by Ametek (1 mentions) Pelco conductive silver paint, by Ted Pella (1 mentions) Leica las v 3, by Leica camera (1 mentions) Quanta 200 feg, by Thermo Fisher Scientific (1 mentions) Ds 5m l1, by Nikon (1 mentions) Eclipse te2000 s, by Nikon (1 mentions) Evo ma15, by Zeiss (1 mentions) Spectramax m2e multi mode microplate reader, by Molecular Devices (1 mentions) Cary 100 uv vis spectrophotometer, by Agilent Technologies (1 mentions) Avance 400 spectrometer, by Bruker (1 mentions) Uv 2550, by Shimadzu (1 mentions) D8 advance diffractometer, by Bruker (1 mentions) Chi 660b, by Chenhua (1 mentions) Tecnai f20, by Thermo Fisher Scientific (1 mentions)

33 protocols using quanta 200 feg sem

Comprehensive Characterization of Cu@ZnO Nanocomposites

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XRD patterns were
recorded for the synthesized composite materials using Rigaku smartlab
in the 2θ range of 20–80°. The surface morphology
of the materials was analyzed using FEI quanta FEG 200 SEM. XPS measurements
were done using an Omicron ECA probe spectrometer, with polychromatic
Mg Kα X-rays (hν = 1253.6 eV). ESR analyses
of the Cu@ZnO nanocomposites were analyzed for oxygen vacancies and
conduction band electrons using JEOL model JES FA200. MALDI-TOF analysis
was done using a Bruker ultraflextreme equipped with a pulsed IR laser,
with 337 nm wavelength; the mass spectra of the Cu@ZnO nanocomposite
materials were recorded in the positive reflection mode. A high resolution
TEM (HRTEM, JEOL model 3010) was used for the identification of cluster
formation and to measure the lattice distance. The band gap of the
composite materials was measured using Jasco 650 along with the spherical
accessory using the principles of DRS.

Krishnamurthi P., Raju Y., Khambhaty Y, & Manoharan P.T. (2017). Zinc Oxide-Supported Copper Clusters with High Biocidal Efficacy for Escherichia coli and Bacillus cereus. ACS Omega, 2(6), 2524-2535.

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3D Reconstruction of Dendritic Spines from SEM

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Serial block-face images are collected using a Gatan 3View system (Gatan) mounted in an FEI Quanta FEG 200 SEM operating at an acceleration voltage of 3 kV with an in-chamber pressure of 10 Pa of water vapor. Samples were imaged at 10 000 × in order to visualize dendritic spines and PSD; the voxel size is 6 × 6 × 50 nm (x-y-z), the latter was chosen as the thickness for the SEM cutting interval. The resulting datasets were assembled into aligned volume files using Digital Micrograph (Gatan) and then semiautomatically or manually segmented and reconstructed in 3D using Ilastik 0.5 or 1.2 (www.ilastik.org) or AMIRA 5.4.3 software package (FEI Visualization Science Group), respectively. 3D models were either measured using the “Material Statistics” module of AMIRA, or Neuromorph addon available for Blender (www.blender.org).

Vezzoli E., Calì C., De Roo M., Ponzoni L., Sogne E., Gagnon N., Francolini M., Braida D., Sala M., Muller D., Falqui A, & Magistretti P.J. (2019). Ultrastructural Evidence for a Role of Astrocytes and Glycogen-Derived Lactate in Learning-Dependent Synaptic Stabilization. Cerebral Cortex (New York, NY), 30(4), 2114-2127.

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Integrated Microscopy Protocol for Multimodal Imaging

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Images where taken on two different integrated microscope systems. For Figs 1 to 3, data was collected on a home-built integrated microscope in an FEI Quanta FEG 200 SEM. The integrated wide-field fluorescence microscope, described in detail by Zonnevylle et al.10 (link), was equipped with a Nikon 60 ×, 0.95NA CFI PLAN APO objective and 200 mm Nikon tube lens, an Andor Clara E camera for Figs 1 and 2 and an Andor Zyla 5.5 camera for Fig. 3. Data for Fig. 4 was collected using a SECOM platform (Delmic, the Netherlands) equipped with a Nikon 40x, 0.95NA CFI PLAN APO objective, installed on an FEI Verios SEM.

Haring M.T., Liv N., Zonnevylle A.C., Narvaez A.C., Voortman L.M., Kruit P, & Hoogenboom J.P. (2017). Automated sub-5 nm image registration in integrated correlative fluorescence and electron microscopy using cathodoluminescence pointers. Scientific Reports, 7, 43621.

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Scanning Electron Microscopy of Desiccated Discs

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One disc from the control group and four discs from each of the experimental groups were desiccated with silica gel for 72 hours. Following dehydration, the discs were mounted on sample holders, gold sputter-coated (2 nm) and examined with an Everhart-Thornley detector in a FEI Quanta FEG 200 SEM at 10 kV under high vacuum (Bio21 Advanced Microscopy Facility, Victoria, Australia). The remaining discs from each group were desiccated using silica gel for 72 hours and examined using SEM-EDS as described for the enamel samples.

Fernando J.R., Shen P., Sim C.P., Chen Y.Y., Walker G.D., Yuan Y., Reynolds C., Stanton D.P., MacRae C.M, & Reynolds E.C. (2019). Self-assembly of dental surface nanofilaments and remineralisation by SnF2 and CPP-ACP nanocomplexes. Scientific Reports, 9, 1285.

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SEM-EDS Analysis of Enamel Remineralization

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SEM-EDS analysis was conducted on TMR sections of enamel lesions treated in vitro by CPP-ACP + SnF₂ to assess the distribution of elements within the lesion following remineralisation. The samples were examined at 10 kV under low vacuum using a solid-state diode backscatter electron detector in a FEI Quanta FEG 200 SEM operating at 10 kV with an energy-dispersive spectrometer (Bio21 Advanced Microscopy Facility, Victoria, Australia). Characteristic x-rays from areas of interest were then detected using an energy dispersive x-ray spectrometer and microanalysis software (AZtec Microanalysis Suite Ver 3.1, Oxford Instruments).

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Scanning Electron Microscopy of Freeze-Dried Samples

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A fragment of freeze-dried gel was attached to a self-adhesive carbon disc mounted on a 25 mm aluminium stub. The stub was coated with 25 nm of gold using a sputter coater. The stub was then placed into a FEI Quanta 200 FEG SEM for imaging at 5 kV accelerating voltage using secondary electron detection.

Joubert F., Cheong Phey Denn P., Guo Y, & Pasparakis G. (2019). Comparison of Thermoresponsive Hydrogels Synthesized by Conventional Free Radical and RAFT Polymerization. Materials, 12(17), 2697.

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Skin Specimen Inoculation and Visualization

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Using the prevention strategy as outlined in Section 2.4, skin specimens were first treated with ABIL and Eudragit, followed by inoculation with microconidia. These samples were then incubated for 24 h at 30 °C and visualized with scanning electron cryo-microscopy. For this, the skin specimens were directly mounted on SEM pin stubs with PELCO conductive silver paint (Ted Pella, Redding, CA, USA) and frozen immediately in liquid nitrogen. The specimens were then coated in a sputter coater and imaged by a Quanta 200 FEG SEM (FEI, Hillsboro, OR, USA).

Ho F.K., Bolhuis A, & Delgado-Charro M.B. (2021). Prevention and Treatment of Fungal Skin Infections Using Cationic Polymeric Films. Pharmaceutics, 13(8), 1161.

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Pectin Hydrogel Bead Characterization

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Pectin hydrogel beads were examined for morphology and the encapsulation of bacteria using scanning electron microscopy (SEM), confocal microscopy, and optical microscopy.
For SEM examination, hydrogel beads were fractured in liquid nitrogen. Sample fragments were mounted on aluminum specimen stubs with colloid silver adhesive and coated with a thin layer of gold by dc sputtering. A Quanta 200 FEG SEM (FEI, Hillsboro, OR) was used to collect images. Images were taken in the high vacuum/secondary electron imaging model at 2,500X and 10,000X.
For optical microscopy, images were collected with a Leica MZ FLIII stereoscopic microscope (SMZ1500, Nikon Instruments Inc., Melvilla, NY) equipped with a DFC420C camera and Leica LAS V. 3.8 software (National Institute of Health, MD).
For confocal microscopy, samples of pectin hydrogel beads containing LGG were placed on microscope slides and observed using an IRBE optical microscope with a 10X lens integrated with a model TCS-SP laser scanning confocal microscope (Leica Microsystems, Exton, PA). The parameters for image acquisition were set at 485/500–530 nm (excitation/emission).

Li R., Zhang Y., Polk D.B., Tomasula P.M., Yan F, & Liu L. (2016). Preserving viability of Lactobacillus rhamnosus GG in vitro and in vivo by a new encapsulation system. Journal of controlled release : official journal of the Controlled Release Society, 230, 79-87.

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Characterization of 3D Printed Punctal Plugs

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DLP 3D printed punctal plugs (D10, D20, D10PEG, D20PEG) were attached to a self-adhesive carbon disc mounted on a 25.0 mm aluminium stub, which was sputter coated with 25.0 nm of gold. The images were captured with an FEI Quanta 200 FEG SEM (FEI, Hillsboro, OR, USA) at 5.0 kV accelerating voltage using secondary electron detection to obtain the images.

Xu X., Awwad S., Diaz-Gomez L., Alvarez-Lorenzo C., Brocchini S., Gaisford S., Goyanes A, & Basit A.W. (2021). 3D Printed Punctal Plugs for Controlled Ocular Drug Delivery. Pharmaceutics, 13(9), 1421.

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Morphological Analysis of Nanofiber Networks

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The morphological properties of nanofiber networks were examined through scanning electron microscopy with an FEI Quanta 200 FEG, using the GSED detector at ESEM mode with 3−10 keV beam energy. After 10 min of gelation on glass surfaces, the nanofibers were treated with gradually increasing ethanol concentrations and then dried using the Tousimis Autosamdri^®-815b critical point dryer. The dried samples were coated with 3 nm Au/Pd and imaged with FEI Quanta 200 FEG SEM under a high vacuum.

Yaylaci S., Dinç E., Aydın B., Tekinay A.B, & Guler M.O. (2023). Peptide Nanofiber System for Sustained Delivery of Anti-VEGF Proteins to the Eye Vitreous. Pharmaceutics, 15(4), 1264.

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