Mts kit

Manufactured by Promega

Sourced in United States, China

The MTS kit is a laboratory equipment product offered by Promega. It is used to measure the viability and metabolic activity of cells in culture. The core function of the MTS kit is to provide a colorimetric method for determining the number of viable cells in proliferation or cytotoxicity assays.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (7 mentions) Rhodamine phalloidin, by Thermo Fisher Scientific (3 mentions) Elx800, by Agilent Technologies (2 mentions) Versamax microplate reader, by Molecular Devices (2 mentions) Milli q water purification system, by Merck Group (2 mentions) Cisplatin, by Merck Group (2 mentions) Curcumin, by Merck Group (2 mentions) Mcroy 5a medium, by Thermo Fisher Scientific (2 mentions) Eagle s minimum essential medium emem, by Thermo Fisher Scientific (2 mentions) Celltiter kit lncap, by Promega (2 mentions) Penicillin, by Merck Group (2 mentions) Streptomycin, by Merck Group (2 mentions) Celltiter 96 aqueous kit, by Promega (1 mentions) Inverted microscope, by Olympus (1 mentions) Envision multilabel reader, by PerkinElmer (1 mentions) Elx808 absorbance reader, by Agilent Technologies (1 mentions) 96 well multiscanner autoreader, by Agilent Technologies (1 mentions) Magic redtm cathepsin b assay kit, by ImmunoChemistry Technologies (1 mentions) Thp 1 cell, by American Type Culture Collection (1 mentions) Phorbol 12 myristate acetate pma, by Merck Group (1 mentions)

98 protocols using mts kit

Cytotoxicity Evaluation of Microneedle Patches

Cited 1 time

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NIH 3T3 fibroblast cells were grown at 37°C in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (Gibco‐Invitrogen Corp., USA). Cultures were maintained in a 95% air/5% carbon dioxide atmosphere, at 95% relative humidity. MN patches were sterilized by exposure to UV light for 15 min. The cytotoxicity of the MN patches was studied by exposing cell lines to 3 × 3 tested needles. Cytotoxicity was assessed 48 h after cells were added, using MTS kits
⁴³ (link) (CellTiter 96 Aqueous kit, Promega, USA) and live/dead fluorescence viability kits
²⁶ (link) (Abcam, England). The results are reported as means ± SD (n = 4).

Freundlich E., Shimony N., Gross A, & Mizrahi B. (2023). Bioadhesive microneedle patches for tissue sealing. Bioengineering & Translational Medicine, 9(3), e10578.

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Cell Proliferation Assay with MTS

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Cell proliferation assays were performed using the MTS kits (Promega, Madison, Wisconsin, USA). Cell suspensions (1000 cells/well) were inoculated in a 96-well plate. After culturing for 24 h, the old mediums were discarded. Mixtures of MTS and medium were added into each well (Reagent: media = 1:4). Optical density (OD) values at 490 nm wavelength were measured after 2 h cultured.

Zhang D., Zhou Y., Ma Y., Jiang P., Lv H., Liu S., Mu Y., Zhou C., Xiao S., Ji G., Liu P., Zhang N., Sun D., Sun H., Wu N, & Jin Y. (2022). Ribosomal protein L22-like1 (RPL22L1) mediates sorafenib sensitivity via ERK in hepatocellular carcinoma. Cell Death Discovery, 8, 365.

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Cell Viability Assay of CNC Extract

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Cell viability was assessed with MTS kits according to the manufacturer instructions (Promega, Madison, WI, USA). Briefly, 3,000 cells/well were seeded in 96-well plates. After overnight incubation, the cells were treated with or without CNC extract (31.25, 46.875, 62.5, 93.75, 125, 187.5, 250 μg/mL) for 48 h prior to the MTS assays. The classic ferrostatin-1 rescue experiment was performed by MTS, the concentration of ferrostatin-1 was 1uM, and the concentration of CNC extract was 100ug/mL. Cells morphology was observed and photographed under an inverted microscope (Olympus, Tokyo, Japan).

Chen Y., Zhang F., Du Z., Xie J., Xia L., Hou X., Hao E, & Deng J. (2021). Proteome Analysis of Camellia nitidissima Chi Revealed Its Role in Colon Cancer Through the Apoptosis and Ferroptosis Pathway. Frontiers in Oncology, 11, 727130.

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Cell Viability and Colony Formation Assay

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After 24 h of transfection, cells were trypsinized and plated into 96-well plates (8000 per well). At different time points (12 h, 24 h, 48 h), cells were determined by using the MTS kits (Promega, WI, USA) following the manufacturer's protocol and the absorption was read at 490 nm. For colony formation assay, cells were trypsinized and plated in 6-well plates (2000 per well) after transfected for 24 h. Colonies were counted 14 days later using Crystal violet fixed.

Xiang Z., Wang S, & Xiang Y. (2014). Up-Regulated MicroRNA499a by Hepatitis B Virus Induced Hepatocellular Carcinogenesis via Targeting MAPK6. PLoS ONE, 9(10), e111410.

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Cell Viability Assay for Adipocytes

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Cell viability was measured by the cell proliferation MTS kit (Promega Corporation, Madison, MI, USA) according to the manufacturer’s protocols. Briefly, the 3T3-L1 adipocytes were treated with various concentrations of adenanthin in 96-well plates for 7 days. The medium was removed, and the MTS solution was added to each well for 2 h. The absorbance was measured at 492 nm by using a microplate reader (Perkin Elmer Envision Multilabel reader).

Hu J., Li X., Tian W., Lu Y., Xu Y., Wang F., Qin W., Ma X., Puno P.T, & Xiong W. (2019). Adenanthin, a Natural ent-Kaurane Diterpenoid Isolated from the Herb Isodon adenantha Inhibits Adipogenesis and the Development of Obesity by Regulation of ROS. Molecules, 24(1), 158.

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Cell Viability Measurement via MTS Assay

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Cell viability was measured using an MTS kit (lot#G971A, Promega). Cells were cultured in a 96‐well plate, and the assay solution was added for an incubation of 1.5 hours in the dark. Amicroplate reader (BioTek ELx800) was used to measure the results at 490 nm.

Zhang Z., Yu X., Zhou Z., Li B., Peng J., Wu X., Luo X, & Yang L. (2019). LMP1‐positive extracellular vesicles promote radioresistance in nasopharyngeal carcinoma cells through P38 MAPK signaling. Cancer Medicine, 8(13), 6082-6094.

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Cell Viability Assessment by MTS Assay

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Cell viability was determined using an MTS kit (Promega Corporation, Madison, WI, USA). SK-N-SH cells were seeded at a density of 4,000 cells/well and treated in 96-well plates for various durations. Following 24 h culture, the cells were washed with PBS three times and fresh DMEM medium (100 µl) and MTS (20 µl) reagent were added to the wells for 1 h at 37°C in the dark (25 (link),26 (link)). The absorbance was measured at a wavelength of 490 nm on an ELx808 absorbance reader (BioTek Instruments, Inc., Winooski, VT, USA). To eliminate possible interference by alcohol, cells treated with the same concentrations of alcohol (0, 50, 100, 200 and 400 mM) and memantine (4 µM) but without addition of assay reagents were used as blank controls. All experiments were repeated at least five times.

Wang H., Wang X., Li Y., Yu H., Wang C., Feng C., Xu G., Chen J., You J., Wang P., Wu X., Zhao R, & Zhang G. (2018). Chronic ethanol exposure induces SK-N-SH cell apoptosis by increasing N-methyl-D-aspartic acid receptor expression and intracellular calcium. Experimental and Therapeutic Medicine, 15(4), 3791-3800.

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Cell Proliferation Assay Using MTS Kit

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The cells were seeded at a density of 5 × 10³ cells/well in a 96-well plate. After the cells were incubated with drugs following the above procedures, the cell proliferation was detected using an MTS kit (CTB169, Promega, WI, USA). The absorbance at 490 nm was read on a microplate reader (51119200, Thermo Fisher Scientific, Waltham, MA, USA) and the relative cell proliferation was calculated according to the formula provided in the kit.

Zhang X., Li X., Xiong G., Yun F., Feng Y., Ni Q., Wu N., Yang L., Yi Z., Zhang Q., Yang Z., Kuang Y., Sai B, & Zhu Y. (2022). Palmitic Acid Promotes Lung Metastasis of Melanomas via the TLR4/TRIF-Peli1-pNF-κB Pathway. Metabolites, 12(11), 1132.

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Evaluating Tyloxapol's Impact on Cell Viability

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Raw 264.7, BMMs and BMSCs cells were pretreated separately with the indicated concentrations of tyloxapol for 48 h. Then, the cellular viability was evaluated utilizing the commercial MTS kit (Promega, Australia) on the basis of the manufacturer’s operating instructions.

Guo W., Li H., Lou Y., Zhang Y., Wang J., Qian M., Wei H., Xiao J, & Xu Y. (2021). Tyloxapol inhibits RANKL-stimulated osteoclastogenesis and ovariectomized-induced bone loss by restraining NF-κB and MAPK activation. Journal of Orthopaedic Translation, 28, 148-158.

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Cell Viability Assay with CA

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Cells were seeded (2 × 10⁴ cell/well) on 96 well plates, stabilized for 24 h, and incubated with various concentrations of CA (10-1000 μg/mL) for additional 48 h. Cell viability was monitored using the cell proliferation 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) kit (Promega Co., Madison, WI, USA) according to the manufacturer’s instructions. The absorbance was measured at 490 nm with a VERSAmax microplate reader (Molecular Devices, Sunnyvale, CA, USA).

Park J., Kim H.L., Jung Y., Ahn K.S., Kwak H.J, & Um J.Y. (2019). Bitter Orange (Citrus aurantium Linné) Improves Obesity by Regulating Adipogenesis and Thermogenesis through AMPK Activation. Nutrients, 11(9), 1988.

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