Sigmafast p nitrophenyl phosphate tablets
SIGMAFAST™ p-Nitrophenyl phosphate Tablets are a colorimetric substrate that can be used to detect and quantify the presence of alkaline phosphatase (ALP) in various assays and applications. The tablets contain p-nitrophenyl phosphate, which is hydrolyzed by ALP to produce a yellow-colored p-nitrophenol product that can be measured spectrophotometrically.
Lab products found in correlation
38 protocols using sigmafast p nitrophenyl phosphate tablets
Enzymatic Assay for Alkaline Phosphatase Activity
Quantitative Alkaline Phosphatase Assay
Osteogenic Differentiation of hSF-MSCs on PEKK/TCP
Alkaline Phosphatase Activity Assay for Osteoblasts and MSCs
Quantifying ADAM17-Mediated Shedding in Cell Lines
Osteogenic Potential of MC3T3-E1 Cells
ELISA for Recombinant NTS-DBL1α Antibodies
Quantifying Plasma C3c Complement Binding
Serum IgE Quantification via ELISA
Quantifying ADAM17-Mediated Shedding in Cell Lines
Forty-eight hours after transfection, AP-HB-EGF HeLa were treated with 160 nM PMA or SFM for 45 minutes (as described18 (link)). Seventy-two hours after transfection, MCF-7 cells were stimulated with SFM, 160 nM PMA or 1 µM ionomycin for 30 minutes (as described27 (link)). Shedding of AP-HB-EGF and knockdown efficiency were analysed as above.
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