Embelin was purchased from Tocris Bioscience (Ellisville, MO). MTT was purchased from Sigma (St. Louis MO, MA). LY294002 and zVAD-fmk was purchased from Calbiochem (San Diego, CA, USA). XIAP antibody was purchased from BD Transduction lab (San Jose, CA, USA). Antibodies against caspase-9, caspase-3, PARP, p-AKT, p-Bad, Bcl-2, Bcl-Xl, Beta-actin, Survivin and Bid were purchased from Cell Signaling Technologies (Beverly, MA, USA). Cytochrome c and GAPDH antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). cIAP-1 antibody was purchased from R&D (USA). Annexin V/PI staining kit was purchased from Molecular Probes (Eugene OR, USA).
Gapdh antibody
Manufactured by Santa Cruz Biotechnology
Sourced in United States, Germany, United Kingdom, France
The GAPDH antibody is a widely used tool in molecular biology research. GAPDH, or Glyceraldehyde 3-phosphate dehydrogenase, is a key enzyme involved in the glycolytic pathway. The GAPDH antibody is commonly used for the detection and quantification of GAPDH protein expression in various biological samples, making it a valuable reagent for Western blotting, immunohistochemistry, and other related applications.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (17 mentions)
Pvdf membrane, by Merck Group (10 mentions)
Cleaved caspase 3, by Cell Signaling Technology (9 mentions)
Bcl 2, by Cell Signaling Technology (8 mentions)
Caspase 3, by Cell Signaling Technology (8 mentions)
Pierce bca protein assay kit, by Thermo Fisher Scientific (7 mentions)
Caspase 9, by Cell Signaling Technology (6 mentions)
Bcl xl, by Cell Signaling Technology (6 mentions)
Ripa lysis buffer, by Beyotime (6 mentions)
Hoechst 33342 solution, by BD (5 mentions)
Cytofix cytoperm plus fixation and permeabilization solution kit, by BD (5 mentions)
Caspase 8, by Cell Signaling Technology (5 mentions)
Annexin 5 fitc, by BD (5 mentions)
β actin antibody, by Santa Cruz Biotechnology (5 mentions)
Cisplatin, by Merck Group (5 mentions)
Nitrocellulose membrane, by Bio-Rad (5 mentions)
Bca protein assay kit, by Thermo Fisher Scientific (5 mentions)
Insulin, by Merck Group (4 mentions)
Polyvinylidene difluoride membrane, by Merck Group (4 mentions)
E cadherin, by Cell Signaling Technology (4 mentions)
213 protocols using gapdh antibody
1
Molecular Pathways of Embelin-Induced Apoptosis
2
Collagen-Rivaroxaban-Edoxaban Signaling Pathway
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Collagen was purchased from Takeda Austria GmbH (Linz, Austria). Rivaroxaban and edoxaban were kindly provided by Bayer Vital GmbH (Leverkusen, Germany) and Daiichi Sankyo Co., Ltd. (Tokyo, Japan), respectively. Phospho-specific p44/p42 MAP kinase antibodies, p44/p42 MAP kinase antibodies, phospho-specific HSP27 antibodies (Ser-78) and phospho-specific HSP27 antibodies (Ser-82) were purchased from Cell Signaling Technology, Inc. (Beverly, MA). HSP27 antibodies and GAPDH antibodies were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). The PDGF-AB enzyme-linked immunosorbent assay (ELISA) kit was obtained from R&D System, Inc. (Minneapolis, MN). The phosphorylated HSP27 ELISA kit was purchased from Enzo Life Science INC. (Farmingdale, NY). ECL Western blotting detection system was obtained from GE Healthcare (Buckinghamshire, UK). Other materials were obtained from commercial sources.
3
Murine Hematopoietic Cytokine Analysis
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Recombinant murine-IL-3 (rm-IL-3), rm-IL-6, rm-SCF and rm-GM-CSF were purchased from eBioscience (San Diego). Recombinant human IL-3, IL-6, SCF, Flt-3, and TPO were obtained from Humanzyme. TNFα was purchased from BD Biosciences. BAY11-7085 and SP600125 small molecule inhibitors were purchased from Millipore. Cell lysis buffer (10×) was obtained from Cell Signaling, and supplemented with proteinase inhibitors and phosphatase inhibitors (Roche Diagnostics). GAPDH antibodies were obtained from Santa Cruz Biotechnology. c-Jun, JunB, and JunD primary and requisite secondary antibodies were also obtained from Cell Signaling. Tri-reagent used for RNA extraction was purchased from Sigma Aldrich. Etanercept® and Kinaret® were graciously provided by the hematology/oncology clinic at Loyola University Chicago. Methylcellulose for CFU assays was purchased from StemCell Technologies. Anti-CD11b and Anti-CD117 antibodies were purchased from eBioscience. Wright-Geimsa solutions were purchased from Exaxol (Clearwater, FL).
4
Western Blot Analysis of AKT, pAKT, ERK, pERK
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HepG2 cells were collected and lysed in RIPA buffer. Protein was loaded onto 10% polyacrylamide gels and transferred to PVDF membranes (Immobilon‐P, Millipore). Membranes were blocked for nonspecific antibody binding in 5% nonfat milk and incubated with the corresponding primary and secondary antibodies. AKT, pAKT, ERK, and pERK antibodies were obtained from Epitomics (Cell Signal Technology, Danvers, USA). GAPDH antibodies were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA).
5
Western Blot Analysis of Apoptosis Regulators
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Western blot analysis was performed as described previously (Wang et al., 2020 (link)). The following antibodies were used: Bcl-2 (#4223), Phospho-Bcl-2 (#2875), Bcl-xL (#2764), Bid (#2002), Bax (#5023), Bad (#9239), phospho-JNK (#4668), JNK (#9252), JNK1 (#3708), JNK2 (#9258), Cleaved Caspase-3 (#9664), PARP (#9532), phospho-c-Jun (Ser73) (#3270), phospho-c-Jun (Ser63) (#91952), c-Jun (#9165), PUMA (#4976), GAPDH (SC-47724). The antibodies were used at either 1:1,000 dilution or 1:2000 dilution. The GAPDH antibodies were purchased from Santa Cruz Biotechnology, and the rest of the antibodies were purchased from Cell Signaling Technology. The band intensity of the target protein was quantified using VisionWork LS image acquisition and analysis software (UVP).
6
Western Blot Analysis of EMT Markers
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SW480 and HCT116 cells were collected and lysed in RIPA buffer. Aliquots (25 μg) of total or nucleus protein were boiled with RIPA buffer and loaded onto 8% or 10% polyacrylamide gels and transferred to PVDF membrane (Immobilon-P, Millipore). Membranes were blocked for nonspecific antibody binding in 5% nonfat milk and incubated with the corresponding primary and secondary antibodies. GAPDH antibodies were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). hTERT, ZEB1, E-cadherin, N-cadherin and vimentin antibodies were obtained from Epitomics (Abcam, Cambridge, UK).
7
Comprehensive Reagent Procurement for Cell Culture
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Crystal violet, sodium dodecyl sulfate (SDS), bovine serum albumin (BSA), insulin, Triton X-100, trypsin, cordycepin, and trypan blue were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Moreover, fetal bovine serum (FBS) was purchased from Life Technologies (Auckland, New Zealand), and dimethyl sulfoxide was purchased from Wako Pure Chemical Industries (Saitama, Japan). Vimentin antibody, E-cadherin antibody, and GAPDH antibodies were purchased from Santa Cruz (Santa Cruz, CA, USA). The PGC-1alpha antibody was obtained from Novus (Littleton, CO, USA). TGF-beta was purchased from Preprotech (London, UK). CD44-FITC antibody and CD117-PE antibody were purchased from (eBioscience, CA, USA). Epidermal growth factor and fibroblast growth factor were purchased from Invitrogen (CA, USA).
8
Quantification of Phosphorylated HSP27 and Akt
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TRAP (H-Ser-Phe-Leu-Leu-Arg-Asn-Pro-Asn-Asp-Lys-Tyr-Glu-Pro-Phe-OH trifluoroacetate salt) was purchased from Bachem AG (Budendorf, Switzerland). Phospho-specific HSP27 (Ser-78) antibodies were purchased from Stressgen Biotechnologies (Victoria, BC, Canada). Phospho-specific Akt (Ser-473 and Thr-308) antibodies, phospho-specific p44/p42 MAP kinase antibodies, phospho-specific p38 MAP kinase antibodies and phospho-specific HSP27 (Ser-82) antibodies were purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA). GAPDH antibodies and deguelin were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). The PDGF-AB ELISA kit was purchased from R&D (Minneapolis, MN, USA). The phosphorylated-HSP27 ELISA kit was purchased from Enzo Life Science, Inc. (Plymouth Meeting, PA, USA). Other materials and chemicals were obtained from commercial sources. Deguelin was dissolved in dimethyl sulfoxide. The maximum concentration of dimethyl sulfoxide was 0.1%, which did not affect the detection of protein expression by a Western blot analysis.
9
Topical Morin Hydrate for Anti-Inflammatory Effects
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Morin hydrate with 90% of purity was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Lecinolws-50 (lysolecithin; Cosphaderm® E145V)) was purchased from Cosphatec GmbH (Hamburg, Germany). Pluronic F-68 non-ionic surfactant was ordered from Sigma (St Louis, MO, USA). Tween-80 (edge activator) was purchased from Shimakyu’s Pure Chemicals (Osaka, Japan). HaCaT keratinocyte cell line was acquired from the I.Z.S.L.E.R. (Institute Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna, Brescia, Italy). Primary antibodies including anti-phospho-ERK 1/2 (Thr202/Tyr204, Thr185/Tyr187) and anti-phospho-p38 (Thr180/Tyr182) were obtained from EMD Millipore Corporation (Temecula, CA, USA). GAPDH antibodies were obtained from Santa Cruz Biotechnology (Dallas, TX, USA) and MMP-1 antibodies were purchased from Proteintech Group, (Rosemont, IL, USA). Fetal Bovine Serum (FBS) and Gibco Dulbecco’s Modified Eagle’s Medium (DMEM) for cell culture were supplied by Thermo Fisher Scientific (Waltham, MA, USA).
10
Immunoblotting and Kinase Assay Protocol
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RAI16 and PKA-RIIα antibodies were from Abcam; 14-3-3θ, Caspase-3, Flag and Myc epitope antibodies were from ABclone; HA and His epitope antibodies were from MBL; HSP70 and GAPDH antibodies were from Santa Cruz Biotechnology; Anti-PKA substrate antibody were from Cell Signaling. RAI16-siRNA, HSP70-siRNA and nonrelative control (NC) siRNA were synthesized by GenePharma (Shanghai, China); FSK were purchased from Sigma; PKImyr, sHt31 were from Promega. Peptide sYF (Aa348-373: YCDHLITEAHTVVADALAKAVAENF) was synthesized by ABclone (Shanghai, China).
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