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Accu check guide glucometer

Manufactured by Roche
Sourced in Switzerland

The Accu-Check Guide glucometer is a portable device used to measure and monitor blood glucose levels. It provides accurate and reliable results to help individuals manage their diabetes.

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2 protocols using accu check guide glucometer

1

Interstitial Fluid Glucose Measurement

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Xenografts were dissected within 2 min after the mice were sacrificed by cervical dislocation. They were weighed, briefly washed in PBS, put on a cell sieve with 20-μm pores (pluriStrainer, 20 μm, Pluriselect) on top of a 50-ml tube, and centrifuged at 300g at 4°C for 10 min. Flow-through interstitial fluid was collected (5 to 100 μl per xenograft). Glucose concentration in the interstitial fluid was measured by the Glucose colorimetric/Fluorometric Assay Kit (Merck), according to the manufacturer’s instructions. Five microliters of interstitial fluid was used per measurement. Blood glucose was measured by tail vein puncture using an Accu-Check Guide glucometer (Roche).
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2

Glucose and Insulin Tolerance Tests

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Mice were fasted for 6 h prior to glucose tolerance test and 4 h prior to Insulin tolerance test, starting at 6:00 a.m. Mice were single-housed during the fasting period and the duration of the test. Water was provided ad libitum. Basal glucose levels were measured before intraperitoneal injection of the glucose or Insulin solution (fixed dose of 42 mg glucose (Sigma-Aldrich, St. Louis, MI, United States), and 0.02 IU Insulin (Novo Nordisk, Bagsvaerd, Denmark)), respectively. Blood glucose levels (mg/dl, measured with Accucheck guide glucometer (Roche, Basel, Switzerland)) were measured after 15, 30, 60, and 120 min of glucose injection. Blood samples were taken from the tail vein.
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