Cumene h2o2

Dexamethasone (Dex), aluminum chloride, folin–ciocalteau reagent, 2,2-diphenyl-1-picrylhydrazyl (DPPH), sodium nitroprusside, Griess reagent (sulphanilamide and N-(1-Naphthyl) ethylenediamine dihydrochloride), tris-HCL, pyrogallol, thiobarbituric acid, trichloroacetic acid, 5,5-dithio-bis-2-nitrobenzoic acid (DTNB), cumene H₂O₂, reduced glutathione (GSH), and phenazine methosulfate fluoride (PMSF) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The total protein assay kit was purchased from BioMED (Cairo, Egypt). Total RNA extraction kit, cDNA synthesis kit, and SYBR green with low ROX were purchased from Enzynomics (Daejeon, Republic of Korea). Primers and antibodies were obtained from Willowfort (Nottingham, UK) and Cell Signaling Technology (Danvers, Massachusetts, USA), respectively. Other chemicals were of analytical grades.

Folin-Ciocalteau reagent, aluminum chloride, gallic acid, catechin, thiobarbituric acid, cumene H₂O₂, 5,5′-dithio-bis-2-nitrobenzoic acid (DTNB), p-nitrobenzyl chloride, reduced glutathione (GSH), pyrogallol, and DEXA were obtained from Sigma-Aldrich (St. Louis, MO, USA). Acid phosphatase (ACP), alkaline phosphatase (ALP), calcium, and total protein assay kits were purchased from Spectrum, Egypt. Osteocalcin (OCN), p38, peroxisome proliferator-activated receptors (PPARγ) and cluster differentiation-90 (CD90) ELISA kits were obtained from INOVA, China. Easy redTM total RNA extraction kit, as well as cDNA synthesis kit and RealMOD™ Green w2 2X qPCR mix kit, were purchased from iNtRON Biotechnology, Korea. Invitrogen, Thermo Fisher Scientific, USA was used to purchase the primers. Other chemicals were obtained with high grades.