Fresh basidiomata of the fungi growing on angiosperm branches were collected from the Qujing, Puer, and Honghe of Yunnan Province, China. The samples were photographed in situ and fresh macroscopic details were recorded. Photographs were taken by a Jianeng 80D camera (Tokyo, Japan). All of the photos were focus stacked and merged using Helicon Focus Pro 7.7.5 software. Specimens were dried in an electric food dehydrator at 40 °C, then sealed and stored in an envelope bag and deposited in the herbarium of the Southwest Forestry University (SWFC), Kunming, Yunnan Province, China.
Focus pro 7
Manufactured by Helicon
The Focus Pro 7.7.5 is a lab equipment product designed for precise and accurate measurements. It features a high-resolution display, intuitive controls, and advanced data processing capabilities. The core function of the Focus Pro 7.7.5 is to provide reliable and consistent measurements for scientific and research applications.
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Lab products found in correlation
Compound microscope, by Zeiss (2 mentions)
Cfi plan 10 0.25na 10 5mm wd objective, by Canon (2 mentions)
Ef 70 200mm f 4l usm zoom lens, by Canon (2 mentions)
Stackshot macro rail, by Helicon (2 mentions)
Eos 1200d, by Canon (2 mentions)
Mp e 65 mm lens, by Canon (2 mentions)
Eos kiss x9, by Nikon (2 mentions)
Bx51 compound microscope, by Canon (1 mentions)
Az100 stereomicroscope, by Nikon (1 mentions)
Eclipse e600 microscope, by Nikon (1 mentions)
Az100, by Nikon (1 mentions)
Eclipse e600, by Nikon (1 mentions)
6 protocols using focus pro 7
1
Fungi Collection from Yunnan, China
2
Collecting and Preserving Fungal Specimens
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Fresh fruiting bodies of the fungi growing on fallen angiosperm branches were collected in 2019 from the Honghe and Wenshan of Yunnan Province, China. The samples were photographed in situ and macroscopic details were recorded. Field photographs were taken by a Jianeng 80D camera (Tokyo, Japan). All photographs were focus-stacked and merged using Helicon Focus Pro 7.7.5 software. Once the macroscopic details were recorded, the specimens were transported to a field station where the specimens were dried on an electronic food dryer at 45 °C. Once dried, the specimens were labeled and sealed in envelopes and plastic bags. The dried specimens were deposited in the herbarium of the Southwest Forestry University (SWFC), Kunming, Yunnan Province, China.
3
Detailed Morphological Documentation of Insects
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Legs and details of the male and female genitalia were drawn by means of an Abbe’s drawing apparatus on a compound microscope (Zeiss Jenaval) at magnification 130–500×. Wings were photographed on an Olympus BX51 compound microscope with an attached digital camera (Canon EOS 1200D). Whole adult (dry-mounted) specimens and heads were photographed by means of a Canon EOS 5D Mark III digital camera with a Nikon CFI Plan 10×/0.25NA 10.5mm WD objective attached to a Canon EF 70–200mm f/4L USM zoom lens. The specimen photographed by means of the latter equipment was repositioned upwards between each exposure using a Cognisys StackShot Macro Rail and the final photograph was compiled from multiple layers (~ 40) using Helicon Focus Pro 7.0.2. The final images were edited in Adobe Photoshop CS6. All morphological illustrations were prepared by the first author.
4
Detailed Microscopic Documentation of Insect Morphology
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Legs were drawn on squared paper using a Reichert binocular microscope with an ocular screen. Details of the male and female genitalia were drawn by means of Abbe’s drawing apparatus on a compound microscope (Zeiss Jenaval) at larger magnification (130–500×). Wings were photographed on the compound microscope Olympus BX51 with an attached digital camera (Canon EOS 1200D). Whole adult (dry-mounted) specimens and wings were photographed by means of a digital camera Canon EOS 5D Mark III with a Nikon CFI Plan 10×/0.25NA 10.5 mm WD objective attached to a Canon EF 70–200 mm f/4L USM zoom lens. The specimen photographed by means of the latter equipment was repositioned upwards between each exposure using a Cognisys StackShot Macro Rail and the final photograph was compiled from multiple layers (20–40) using Helicon Focus Pro 7.0.2. The final images were edited in Adobe Photoshop CS6.
5
New Metapone ant species described
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Types. Metapone quadridentata SKYC ) and one paratype queen (MNHAH ).
Source images of the new species for focus stacking were taking using a Canon EOS Kiss X9 digital camera, attached to a Nikon AZ100 stereomicroscope (for worker, queen, and male bodies, excluding male genitalia), and a Nikon Eclipse E600 microscope (for male genitalia). Focus-stacked images were produced using Helicon Focus Pro 7.0.2 (Helicon Soft Ltd.,http://www.heliconsoft.com/ ), and improved with the retouching function of the same software. Colour balance and contrast were adjusted using GIMP 2.8 (The GIMP Development Team, http://www.gimp.org ). Two paratype workers of M. quadridentata http://zerenesystems.com/cms/stacker ). The final images were further adjusted, annotated, and scale bars added using Adobe Photoshop CS6. All specimens of the new species examined are deposited in the Lee Kong Chian Natural History Museum, under the Zoological Reference Collection (ZRC ).
High resolution image plates are available athttps://doi.org/10.5061/dryad.9776725
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Source images of the new species for focus stacking were taking using a Canon EOS Kiss X9 digital camera, attached to a Nikon AZ100 stereomicroscope (for worker, queen, and male bodies, excluding male genitalia), and a Nikon Eclipse E600 microscope (for male genitalia). Focus-stacked images were produced using Helicon Focus Pro 7.0.2 (Helicon Soft Ltd.,
High resolution image plates are available at
6
Imaging Procedures for Ant Species Identification
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NHMW Naturhistorisches Museum Wien, Vienna, Austria OUMNH Oxford University Museum of Natural History, Oxford, UK SKYC
ZRC Zoological Reference Collection, Lee Kong Chian Natural History Museum, Singapore Source images for focus stacking were taken using a Canon EOS Kiss X9 digital camera, attached to a Nikon AZ100 stereomicroscope (for O. litoralis http://www.heliconsoft.com/ ), and improved with the retouching function of the same software. Colour balance and contrast were adjusted using GIMP 2.8 (The GIMP Development Team, http://www.gimp.org ).
Workers ofO. malignus https://zerenesystems.com/cms/stacker ). All final images were further adjusted, annotated, and scale bars added using Adobe Photoshop CS6.
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Workers of
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