Insulin tolerance tests were performed on 5 h-fasted mice injected intraperitoneally with 1.2 U/Kg human insulin (Humulin R, Eli Lilly). Blood glucose levels were measured with an Accu-Chek glucometer (Roche Diagnostics Corp.) immediately before and 15, 30, 60, 90, and 120 min after glucose injection. Area under the curve values were calculated with the GraphPad Prism software (GraphPad Software Inc.). Fasting glucose levels were obtained after a 16-h fast with an Accu-Chek glucometer (Roche Diagnostics Corp.). Tissue weights were recorded at the time of euthanasia.
Accu chek glucometer
Manufactured by Roche
Sourced in Switzerland, Germany, Spain, United States, United Kingdom, Australia, China, India
The Accu-Chek glucometer is a portable device used to measure blood glucose levels. It provides a quick and accurate reading of the user's current blood sugar concentration.
Automatically generated - may contain errors
Lab products found in correlation
Humulin r, by Eli Lilly (5 mentions)
Streptozotocin (stz), by Merck Group (5 mentions)
D glucose, by Merck Group (4 mentions)
Accu chek active, by Roche (3 mentions)
Insulin, by Merck Group (3 mentions)
Pyruvate, by Merck Group (2 mentions)
Ultra sensitive mouse insulin elisa kit, by Crystal Chem (2 mentions)
Insulin, by Mercodia (2 mentions)
Leptin, by R&D Systems (2 mentions)
Human insulin, by Novo Nordisk (1 mentions)
Actrapid penfill, by Novo Nordisk (1 mentions)
Hydroxyproline colorimetric assay kit, by Merck Group (1 mentions)
Ultrasensitive mouse insulin enzyme linked immunosorbent assay kit, by Crystal Chem (1 mentions)
Infinity cholesterol kit, by Thermo Fisher Scientific (1 mentions)
Multiconstituent calibrator 1e65 04, by Abbott (1 mentions)
Au480 clinical chemistry analyzer, by Beckman Coulter (1 mentions)
Triglyceride quantification colorimetric fluorometric kit, by Abcam (1 mentions)
Bovine insulin, by Merck Group (1 mentions)
Novolin, by Novo Nordisk (1 mentions)
Ezrl 83k, by Merck Group (1 mentions)
116 protocols using accu chek glucometer
1
Insulin Tolerance Test in Fasted Mice
2
Insulin Tolerance Test in Fasted Mice
3
Glucose and Insulin Tolerance Tests
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Glucose tolerance test (GTT) and insulin tolerance test (ITT) were measured during the last week of HFD. For GTT, mice were fasted overnight with free access to water, followed by an oral administration of glucose (2 g/kg). The blood samples were collected from the caudal vein and the blood glucose concentration was measured by using Roche ACCU-CHEKTM glucometer at 0, 15, 30, 60, 90, 120 min. For ITT, mice were fasted for 4 h followed by an intraperitoneal injection of human insulin (1 U/kg). Blood glucose concentration was measured at 0, 15, 30, 45, 60, 120 min and the results were expressed as the change of blood glucose relative to the basal level. The area under the curve (AUC) of GTT or ITT was calculated from the area between the base line and the indicated line using GraphPad software.
4
Fasting Glucose and Serum Cytokines in Mice
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The fasting plasma glucose concentration of mice fasted overnight was measured by using Roche ACCU-CHEKTM glucometer. Blood samples were collected before the mice were sacrificed and serum was prepared by centrifuging at 3000 rpm for 10 min. Serum cytokines leptin (FineTest, P0191), Adiponectin (FineTest, EM001), TNF-α (Biotech, EM008), IL-1β (Biotech, EM001), IL-6 (Biotech, EM004) was evaluated by ELISA kit following standard protocol.
5
Broccoli Juice for Diabetes
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Broccoli was purchased from a local store in Nanjing city, Jiangsu Province, China, then add ultra-pure water for juiced, the ACCU-CHEK glucometer were obtained from Roche (Mannheim, Germany); Human insulin was obtained from Novo Nordisk (Copenhagen, Denmark); Beijing Solarbio Biological Technology Co., Ltd. provided the metformin, while Nanjing Jiancheng Bioengineering Institute provided the commercial kits (Nanjing, China).
6
Streptozotocin-Induced Diabetic Rat Model
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Streptozotocin (STZ) was purchased from Sigma–Aldrich, USA; Biochemical Kits for measuring TC, TG, LDL-c and HDL-c were obtained from Roche Co., Ltd; a sample of glipizide was accepted from Zydus Research Centre, Ahmedabad, India. Accu-chek glucometer (Roche) was already available from previous experiments. Rat insulin ELISA Kit was purchased from GenX biotech and other reagents were procured from Himalayan Scientific House, Chandigarh, India. High Fat diet was prepared in house from coconut oil (Parachute brand) and Vanaspati ghee (Dalda brand), the common dietary ingredients of the Indian diet.
7
Streptozotocin-Induced Diabetes in Rats
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T2DM was induced by a month-long high-fat diet (HFD) (48% carbohydrate, 32% fat and 20% protein), followed by an intraperitoneal injection of 35 mg/kg BW single dose Streptozotocinin 0.1 M sodium citrate buffer, at pH 4.5. One week after injection, BG levels were obtained from the orbital sinus (1-2 drops) to determine a diagnosis of diabetes. An Accu-Chek glucometer (Roche, Germany) was used to determine BG. Rats with BG levels of ≥ 250 mg/dL were classified as diabetic. Moreover, we excluded rats with BG levels of ≤ 250 mg/dL.
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T2DM was induced by a month-long high-fat diet (HFD) (48% carbohydrate, 32% fat and 20% protein), followed by an intraperitoneal injection of 35 mg/kg BW single dose Streptozotocinin 0.1 M sodium citrate buffer, at pH 4.5. One week after injection, BG levels were obtained from the orbital sinus (1-2 drops) to determine a diagnosis of diabetes. An Accu-Chek glucometer (Roche, Germany) was used to determine BG. Rats with BG levels of ≥ 250 mg/dL were classified as diabetic. Moreover, we excluded rats with BG levels of ≤ 250 mg/dL.
8
Streptozotocin-Induced Diabetes in Mice
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Diabetes was induced in mice fasted overnight by a single intraperitoneal (ip) injection of streptozotocin (STZ; Sigma, St. Louis, MO, USA) in a dose of 45 mg/kg. The STZ was dissolved in citrate buffer as the vehicle (0.1 M, pH 4.5) before use for 15 min, then nicotinamide (110 mg/kg BW in normal physiological saline) was administered [10 (link)]. Finally, a solution of 5% glucose was provided to prevent hypoglycemia. Fasting blood glucose levels were measured for 72 h and then the STZ injection was administered with a glucometer in the blood drawn from the tail vein. Diabetes was confirmed in the mice with fasting blood glucose above 250 mg/dl (13.8 mM), which was measured by Accu-Chek Glucometer (Accu-Chek® Active, Roche Diagnostics GmbH, Hannheim, Germany) and expressed in terms of mg/dl.
9
Glucose Tolerance Test Protocol
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During glucose tolerance tests, all mice were fasted for 4 h prior to intraperitoneal injections of dextrose (2 g/kg BW). Tail vein blood glucose levels were measured using Accu Chek Glucometer (Roche Diagnostics, Indianapolis, IN) at 0, 15, 30, 45, 60, 90, and 120 min post glucose administration. Area under the curve over 120 min was calculated.
10
Measuring Blood Glucose and Metabolites
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Blood glucose concentration was measured with an Accu-Chek Glucometer (Roche Diagnostics, Barcelona, Spain). Plasma or serum non-esterified fatty acid (NEFA) and β-hydroxybutyrate concentrations were measured using commercial enzymatic colorimetric kits (from, respectively, Wako Chemicals, Neuss, Germany; and Ben S.r.l. Biochemical Enterprise, Milano, Italy) according to the manufacturer’s instructions.
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