Raman microscope

Manufactured by Renishaw

Sourced in United Kingdom

The Raman microscope is a scientific instrument designed for the analysis of materials. It utilizes Raman spectroscopy, a technique that measures the inelastic scattering of light to provide information about the molecular structure and composition of samples. The core function of the Raman microscope is to capture detailed chemical and structural data from microscopic regions of a specimen.

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Lab products found in correlation

K alpha, by Thermo Fisher Scientific (5 mentions) Jem 2100f, by JEOL (3 mentions) Nicolet 6700, by Thermo Fisher Scientific (3 mentions) Jem 2010f, by JEOL (2 mentions) Tecnai g2 f20 electron microscope, by Thermo Fisher Scientific (2 mentions) Fluoromax 3 spectrofluorometer, by Hamamatsu Photonics (2 mentions) Uv vis spectrometer, by Agilent Technologies (2 mentions) Zetasizer nano z, by Malvern Panalytical (2 mentions) D8 advance, by Bruker (2 mentions) Jsm 6700f, by JEOL (2 mentions) Lsm 780, by Zeiss (2 mentions) Pyris diamond tg dta, by PerkinElmer (1 mentions) Tecnai g2 20, by JEOL (1 mentions) Jem 2011, by JEOL (1 mentions) Eclipse lv100, by Nikon (1 mentions) Uv 1800 photospectrometer, by Shimadzu (1 mentions) Tecnai g2 f20, by Hitachi (1 mentions) Su8020, by Hitachi (1 mentions) X pert pro super x ray diffractometer, by Philips (1 mentions) Supra 40, by Hitachi (1 mentions)

75 protocols using raman microscope

Comprehensive Structural Analysis of CoFe2O4 Nanoparticles

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X-ray diffraction (XRD) patterns of all samples were collected in the range 10–80° (2θ) using a RigakuD/MAX 2550 diffract meter (Cu K radiation, λ = 1.5406 Å), operated at 40 kV and 100 mA. The morphologies were characterized by transmission electron microscopy (TEM, JEM2000EX). The particle size distribution curve was derived from 100 CoFe₂O₄ nanoparticles. The surface morphologies were observed by scanning electron microscopy (TESCAN nova Ш) and field emission scanning electron microscopy (FESEM, NOVA NanoSEM450). Raman measurements were performed at room temperature using Raman microscopes (Renishaw, UK) under the excitation wavelength of 532 nm. BET surface area measurements were carried out by N₂ adsorption at 77 K using an ASAP2020 instrument. Thermogravimetric and differential thermal analyses were conducted on a Pyris Diamond TG/DTA (PerkinElmer) apparatus at a heating rate of 20 K min⁻¹ from 40 to 800 °C in air flow.

Qiu B., Deng Y., Du M., Xing M, & Zhang J. (2016). Ultradispersed Cobalt Ferrite Nanoparticles Assembled in Graphene Aerogel for Continuous Photo-Fenton Reaction and Enhanced Lithium Storage Performance. Scientific Reports, 6, 29099.

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Comprehensive Material Characterization

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The product morphology and crystal structure was examined using field-emission scanning electron microscopy (FESEM; Hitachi, S5500), transmission electron microscopy (TEM; FEI, Tecnai G² 20, 200 kV; JEOL, JEM-2011, 200 kV; JEOL, JEM-2010F, 200 kV), thermal gravimetric analysis (Netzsch-STA 449C, measured from room temperature to 800°C at a heating rate of 10°C min⁻¹ under an air flow), and X-ray photospectroscopy (XPS; Escalab 250, Al Kα, binding energies are referenced to the C 1s of carbon contaminants at 284.6 eV). Crystallographic information for the samples was collected using a Bruker Model D8 Advance X-ray powder diffractometer (XRD) Cu-Kα irradiation (λ = 1.5418 Å). Raman spectra were collected by using Raman microscopes (Renishaw, UK) under a 488 nm excitation. Fourier transform infrared spectra (FT-IR) spectra were recorded with a Nicolet 205 FTIR spectrometer using the KBr pellet technique.

Zhao Y., Kuai L., Liu Y., Wang P., Arandiyan H., Cao S., Zhang J., Li F., Wang Q., Geng B, & Sun H. (2015). Well-Constructed Single-Layer Molybdenum Disulfide Nanorose Cross-Linked by Three Dimensional-Reduced Graphene Oxide Network for Superior Water Splitting and Lithium Storage Property. Scientific Reports, 5, 8722.

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Crystallographic Analysis of Polymorphs

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The morphologies and colour of the crystals precipitated on glass slides were analysed using optical microscopy with a Nikon Eclipse LV100 polarising microscope equipped with transmitted and reflected light sources. Routine identification of crystal polymorph was carried out using Raman microscopy and PXRD. Raman spectra were recorded using a Renishaw Raman Microscope equipped with a 785 nm laser, while laboratory PXRD experiments were carried out using a PANalytical X’Pert^{3 (link)} diffractometer equipped with a Cu-anode (λ = 1.54056 Å).

Kim Y.Y., Darkins R., Broad A., Kulak A.N., Holden M.A., Nahi O., Armes S.P., Tang C.C., Thompson R.F., Marin F., Duffy D.M, & Meldrum F.C. (2019). Hydroxyl-rich macromolecules enable the bio-inspired synthesis of single crystal nanocomposites. Nature Communications, 10, 5682.

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Synthesis and Spectroscopic Analysis of Mn(terpy) Complexes

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The syntheses of [Mn(terpy)Cl 3 ], [Mn(terpy)F 3 ], and [Mn(terpy) (N 3 ) 3 ] were carried out according to literature procedures. [14] [15] [16] The ultraviolet-visible spectra were measured using a Shimadzu UV-1800 photospectrometer using a 1 cm quartz cuvette. Raman spectra were measured using a Renishaw Raman microscope with a laser wavelength of 785 nm.

Barlow K., Eng J., Ivalo I., Coletta M., Brechin E.K., Penfold T.J, & Johansson J.O. (2022). Photoinduced Jahn-Teller switch in Mn(III) terpyridine complexes. Dalton transactions (Cambridge, England : 2003), 51(28).

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Characterization of Au and Au@Ag Nanorods

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The Au nanorods and Au@Ag nanorods were observed by scanning electron microscopy (SEM, SU8020, Hitachi, Japan) and transmission electron microscopy (TEM, Tecnai G2 F20, American). The UV-Vis-NIR spectra were measured on a Shimadzu UV3600-MPC3100 photometer (Shimadzu, Japan). The Raman spectra of the samples were measured and collected using a confocal Raman microscope (Renishaw inVia Reflex, England) equipped with a monochromatic laser light source (633 nm; 785 nm) and 50× long focus objective. An X-ray photoelectron spectrometer (ESCALAB 250Xi, England) was used to measure the XPS spectrum on the surface of the gold and silver samples.

Hu M., Huang Z., Liu R., Zhou N., Tang H, & Meng G. (2022). SERS spectral evolution of azo-reactions mediated by plasmonic Au@Ag core–shell nanorods. Nanoscale Advances, 4(22), 4730-4738.

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Raman Spectra of Materials

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Raman
spectra of the materials were obtained
using a confocal Raman microscope (Renishaw, UK) within a frequency
range of 100–2000 cm^–1.

Mobin M, & Ansar F. (2022). Polythiophene (PTh)–TiO2–Reduced Graphene Oxide (rGO) Nanocomposite Coating: Synthesis, Characterization, and Corrosion Protection Performance on Low-Carbon Steel in 3.5 wt % NaCl Solution. ACS Omega, 7(50), 46717-46730.

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Comprehensive Materials Characterization Protocol

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The achieved samples were examined by multiple analytic techniques. XRD was taken with a Philips X’Pert Pro Super X-ray diffractometer with Cu Kα radiation (λ = 1.54178 Å). The morphology of the samples was investigated by SEM (Zersss Supra 40) and TEM (Hitachi H7650). The STEM and HRTEM images, SAED, and EDX elemental mappings were taken on a JEMARM 200F Atomic Resolution Analytical Microscope with an acceleration voltage of 200 kV. Raman spectra were measured on a Raman microscope (Renishaw®) excited with a 514 nm excitation laser. ICP-AES data were obtained by an Optima 7300 DV instrument. N₂ adsorption/desorption isotherms were recorded on an ASAP 2020 accelerated surface area and a porosimetry instrument (Mictromeritics), equipped with an automated surface area, at 77 K using Barrett–Emmett–Teller calculations. Ultraviolet photoelectron spectroscopy was carried out at the BL11U beamline of National Synchrotron Radiation Laboratory in Hefei, China. The X-ray absorption spectra of Co and Se K-edges were obtained at the beamline 14W1 of Shanghai synchrotron Radiation Laboratory (China). XPS was taken on an X-ray photoelectron spectrometer (ESCALab MKII) with an X-ray source (Mg Kα hυ = 1253.6 eV). The X-ray absorption spectra of Co L-edges were performed on the BL10B beamline of National Synchrotron Radiation Laboratory in Hefei (China).

Zhang X.L., Hu S.J., Zheng Y.R., Wu R., Gao F.Y., Yang P.P., Niu Z.Z., Gu C., Yu X., Zheng X.S., Ma C., Zheng X., Zhu J.F., Gao M.R, & Yu S.H. (2019). Polymorphic cobalt diselenide as extremely stable electrocatalyst in acidic media via a phase-mixing strategy. Nature Communications, 10, 5338.

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Raman Analysis of TDR SSM Samples

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Raman microscopic technique is applied in the present research for chemical investigation of the TDR SSM before (raw TDR) and after (1 st and 9 th week TDR SSM samples) the treatment.
Further, MB simulated wastewater sample was also examined by Raman. All Raman spectra were recorded using a Raman Microscope (RENISHAW in via confocal Raman Microscope, STFC Rutherford Appleton Laboratory [RAL], UK). The Raman signals were collected in the spectral interval 600-2000 cm -1 ; at 830nm wavelength laser source with integration time of 10 s. The experiments were performed at 5X objective extended scan using 50 pc power and 5 accumulations.

Naresh Yadav D., Naz I., Anand Kishore K, & Saroj D. (2021). Evaluation of tire derived rubber (TDR) fixed biofilm reactor (FBR) for remediation of Methylene blue dye from wastewater. Environmental technology, 42(23).

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SERS Characterization of R6G in ISF and Serum

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Raman spectra were collected using an inVia confocal Raman microscope (Renishaw, Gloucestershire, UK) mounted on Leica microscope and controlled with Wire 3.4 software. The 785 nm wavelength diode laser (0.5mW) coupled to a holographic notch filter with a grafting of 1200 lines mm⁻¹ was focused onto the sample using a 20X objective (NA = 0.4) with 10s exposure time., and 1 accumulation was collected per spot. Six spectra were collected from different spots across each substrate, using a motorized XYZ translational stage integral to the microscope.
ISF calibrators were prepared by adding aliquots of R6G stock solution to ISF (extracted from porcine cadaver skin) to create the following concentrations: 0, 0.05, 0.5, 2, 5, 10, 25, 50 and 100 μM R6G in ISF. Serum calibrators were prepared by adding aliquots of R6G stock solution to rat serum (collected from rats in vivo) to create the following concentrations: 0, 0.5, 5, 10, 25, 50 and 100 μM of R6G in Serum. The plasmonic paper was soaked in 150 μL of calibrator for 1 hour, thoroughly rinsed in DI water for 5 min, and air dried prior to collecting SERS spectrum from six different locations across the substrate.

Kolluru C., Gupta R., Jiang Q., Williams M., Derami H.G., Cao S., Noel R.K., Singamaneni S, & Prausnitz M.R. (2019). Plasmonic paper microneedle patch for on-patch detection of molecules in dermal interstitial fluid. ACS sensors, 4(6), 1569-1576.

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Raman Spectroscopy of Nanomaterials

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Raman spectra were acquired using an inVia confocal Raman microscope (Renishaw). The three types of nGO (bare, nGO-PEG-and nGO-PEtOx) with or without plasma protein coatings, as well as the plasma protein control in aqueous solution (0.5 mg/mL), were mounted on microscope slides and air-dried. The dried samples were excited by a laser line at 785 nm, with a power of 500 mW. Spectra were stored with in-built WiRE 2.0 software.

Wang M., Gustafsson O.J.R., Siddiqui G., Javed I., Kelly H.G., Blin T., Yin H., Kent S.J., Creek D.J., Kempe K., Ke P.C, & Davis T.P. (2018). Human plasma proteome association and cytotoxicity of nano-graphene oxide grafted with stealth polyethylene glycol and poly(2-ethyl-2-oxazoline). Nanoscale, 10(23).

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