Twenty days after the DHEA treatment, the ovaries were immediately sampled from rats (n = 3 per group) under anesthesia and deep-frozen. Immunoblot analysis was performed as previously described [22] . The following primary antibodies were used for Immunoblot analysis: Rabbit anti-Bad (1:500, Cell Signaling Technology, Beverly, MA), mouse anticleaved caspase-9 (1:1000, Cell Signaling Technology), rabbit anticleaved caspase-3 (1:500; Cell Signaling Technology), rabbit anti-p-IκBα, rabbit anti-p–NF-κB p65 (1:1,500; Cell Signaling Technology), rabbit antinuclear factor erythroid 2-related factor 2 (Nrf2; 1:1500, Santa Cruz Biotechnology, Santa Cruz, CA), mouse antiheme oxygenase-1 (HO-1; 1:1,500; Enzo Life Sciences, Farmingdale, NY), mouse anti-NQO1 (1:1,500; Cell Signaling Technology), rabbit antihistone H3 (1:5,000; Cell Signaling Technology), rabbit anticyclooxygenase-2 (COX-2) (1:1,000, Santa Cruz Biotechnology), rabbit anti-iNOS (1:1,000, Santa Cruz Biotechnology), mouse anti-ER alpha (ERα) (1:1,000, Santa Cruz Biotechnology), and mouse anti-ERß (1:500, Santa Cruz Biotechnology) were used as primary antibodies. Immunoblot images were quantified using Image J analysis software (JAVA image processing program, NIH, Bethesda, MD).
Mouse anticleaved caspase 9
Manufactured by Cell Signaling Technology
Sourced in United States
Mouse anti-cleaved caspase-9 is a monoclonal antibody that recognizes the large fragment (p35) of caspase-9 resulting from its cleavage. Caspase-9 is a key enzyme involved in the initiation of the intrinsic apoptosis pathway.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti histone h3, by Cell Signaling Technology (1 mentions)
Rabbit anti inos, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Mouse anti cleaved caspase 8, by Cell Signaling Technology (1 mentions)
Mouse anti s6, by Cell Signaling Technology (1 mentions)
Bez235, by Selleck Chemicals (1 mentions)
Mouse anti beclin 1, by Cell Signaling Technology (1 mentions)
Mouse anti lc3, by Cell Signaling Technology (1 mentions)
Mouse anti mtor, by Cell Signaling Technology (1 mentions)
Mouse anti ps6, by Cell Signaling Technology (1 mentions)
Anti mouse cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Mouse anti pakt, by Cell Signaling Technology (1 mentions)
2 protocols using mouse anticleaved caspase 9
1
Ovarian Protein Expression Analysis
2
Apoptosis and Autophagy Regulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
BEZ235, TSA and 3-MA were purchased from Selleck company (Selleck, Shanghai, CHINA). Antibodies for western blotting and flow cytometry were: mouse anti-cleaved caspase-3, mouse anti-cleaved caspase-8, mouse anti-cleaved caspase-9, mouse anti-p-Akt, mouse anti-Beclin-1, mouse anti-LC3, mouse anti-S6, mouse anti-p-S6, mouse anti-4EBP1, mouse anti-p-4EBP1, mouse anti-mTOR (Cell Signaling, USA), mouse anti-PARP-1 (Abcam, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!