Silastic tubing

Manufactured by Dow

Sourced in United States

Silastic tubing is a flexible, transparent silicone-based material commonly used in laboratory equipment. It is designed to provide a durable, inert, and chemically resistant conduit for the transfer of liquids and gases in various scientific applications.

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Lab products found in correlation

38 protocols using silastic tubing

Jugular Vein Catheterization in Rats

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Animals were anesthetized with ketamine (87.5 mg/kg, i.p.), and xylazine (5 mg/kg, i.p.) administered in a volume of 1 mL/kg. Catheters (SILASTIC tubing, ID 0.51 mm, OD 0.94 mm, Dow Corning, Midland, MI) were implanted and secured into the jugular vein with suture thread (Surgical Specialties Corp., Wyomissing, PA). The catheter tubing was passed subcutaneously and exited from the back where it was connected to a cannula (Plastics One, Roanoke, VA, USA) embedded in a rubber harness (Instech, Plymouth Meeting, PA, USA). This harness was worn by the rat for the duration of the experiment. Carprofen (5 mg/kg, subcutaneous) was administered on the day of surgery and for the three following days for analgesia. Heparin (100 units/mL in 0.1 mL) was administered for the duration of the experiment. Catheter patency was tested periodically with methohexital sodium (10 mg/mL; Eli Lilly, Indianapolis, IN, USA).

Giorgione J., Epand R.M., Buda C, & Farkas T. (1995). Role of phospholipids containing docosahexaenoyl chains in modulating the activity of protein kinase C.. Proceedings of the National Academy of Sciences of the United States of America, 92(21), 9767-9770.

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Hormone Replacement in Gonadectomized Rats

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All rats (16 male, 18 female) were gonadectomized under ketamine/xylazine anesthesia, and half received hormone replacement (n=7–9/group) as in our previous studies (Antzoulatos et al., 2011 ; Kent et al., 2013 (link)). Hormone implants were made from Silastic tubing (id: 1.57 mm, od: 3.18 mm, Dow Corning, Midland, MI) filled with crystalline steroid to provide chronic replacement of testosterone or estradiol at physiologic levels (Moger, 1976 (link); Bridges, 1984 ). Males were castrated via a mid-line scrotal incision, and received a 10mm Silastic implant s.c. either filled with testosterone (ORCHX+T) or blank (ORCHX). These testosterone implants maintain serum testosterone at the level of intact adult male rats (~2.5 ng/ml) (Moger, 1976 (link); Damassa et al., 1977 (link)). Females were ovariectomized via bilateral dorsal flank incisions and received a 5mm implant s.c. filled with 17β-estradiol (OVX+E) or blank (OVX). These estradiol implants maintain serum 17β-estradiol at 50–100 pg/ml, corresponding to periovulatory estrogen levels during proestrus in rats (Becker et al., 2005 (link); Mosquera et al., 2015 (link)). Rats were allowed to recover from surgery for at least 2 weeks before behavioral testing. This time-course is sufficient to demonstrate decreases in hormone-driven behaviors (e.g., mating) after gonadectomy (Hull et al., 2006 ).

Wallin-Miller K.G., Chesley J., Castrillon J, & Wood R.I. (2017). Sex differences and hormonal modulation of ethanol-enhanced risk taking in rats. Drug and alcohol dependence, 174, 137-144.

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Ovariectomy and Estradiol Replacement in Mice

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Adult female mice were anesthetized using 2.5% isoflurane in oxygen delivered by a nose cone. After receiving a preoperative dose of carprofen (5 mg/kg), ovaries were removed through bilateral lumbar incisions. The vasculature to the ovary and body wall were sutured, and wound clips were used to close the incision. For surgeries involving estradiol (E₂) replacement, an E₂-filled capsule was implanted in the interscapular region immediately after OVX surgery. The E₂ implants were made of Silastic tubing (0.59 inches long, 0.078 inches inner diameter, 0.125 inches outer diameter; Dow Corning) and filled with a low dose of crystalline E₂ (20 μg/mL, in sesame oil) as previously described (Navarro et al., 2015 (link)).

Heppner K.M., Baquero A.F., Bennett C.M., Lindsley S.R., Kirigiti M.A., Bennett B., Bosch M.A., Mercer A.J., Rønnekleiv O.K., True C., Grove K.L, & Smith M.S. (2017). GLP-1R Signaling Directly Activates Arcuate Nucleus Kisspeptin Action in Brain Slices but Does not Rescue Luteinizing Hormone Inhibition in Ovariectomized Mice During Negative Energy Balance. eNeuro, 4(1), ENEURO.0198-16.2016.

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Silastic Capsules for Sex Steroid Replacement

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Silastic capsules were utilized for sex steroid replacement. Capsules consisted of a 10 mm long section of Silastic tubing (Dow Corning, Midland, MI) filled with either 17β‐estradiol (Sigma‐Aldrich, St. Louis, MO), cholesterol (MP Biomedicals, Solon, OH), or a mixture of the two chemicals. Control animals received capsules filled with 100% cholesterol; experimental groups received capsules filled with a 10:90 mixture of 17β‐estradiol and cholesterol, a 50:50 mixture of 17β‐estradiol and cholesterol, or 100% 17β‐estradiol. Prepared capsules were sealed at both ends with weatherproof silicone caulk, washed in 70% ethanol, and rinsed with sterile water. Capsules containing cholesterol were stored at −20°C until use; capsules containing 100% 17β‐estradiol were stored at room temperature until use.
Capsules were surgically implanted between the skin and fascia on the dorsum of the animals. Briefly, a small incision was made on the dorsolateral aspect of the neck. Hemostats were used to create a pocket between the skin and muscle tissue and the capsule was placed inside the formed cavity. The skin incision was closed with a wound clip. All surgeries were performed under light isoflurane anesthesia and with sterile techniques as described above. All animals were allowed a 2‐day postprocedure recovery period to facilitate efficient release of the steroid from the capsule.

Cates B.E., Dillard B.M., Foster B.R., Patterson S.V., Spivey T.P., Combs E.B, & Bowen R.S. (2018). Effects of varying doses of estrogen and caudal pressure on wheel running in orchidectomized male mice. Physiological Reports, 6(11), e13730.

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Estradiol Microimplantation in Ovariectomized Rats

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Rats were ovariectomized (OVX), and some of them received unilateral estradiol (Sigma-Aldrich, St. Louis, MO, USA) or cholesterol microimplants in the various hypothalamic regions in the pre- (day 20 of age) or postpubertal period (day 35 of age). The microimplants were made of a 1:1000 (w/w) mixture of estradiol and paraffin. Cholesterol was used instead of estradiol in controls. The paraffin mixture (approximately 0.05 mm³), theoretically containing 33 ng estradiol or cholesterol, was then punched out with a 24-gauge stainless steel tubing (0.30 mm i.d.). The stainless steel tubing containing the paraffin mixture was then stereotaxically inserted in the discrete area of the hypothalamus. The paraffin mixture was immediately pushed out of the tubing and into the designated nucleus using a stainless steel wire (0.25 mm diameter). The stereotaxic coordinates (mm posterior and ventral to the bregma, and lateral to the midline) were as follows: mPOA, 0.8, 7.7, 0.4;
PVN, 1.8, 7.4, 0.5; VMH, 2.8, 9.0, 0.8; and ARC, 2.8, 9.0, 0.5.
Some of the OVX animals were subcutaneously implanted with a Silastic tubing (1.57 mm i.d.; 3.18 mm o.d.; 8 mm in length for day 20 and 16 mm in length for day 35, Dow Corning, Midland, MI, USA) containing estradiol dissolved in peanut oil at 20 μg/ml to mimic the diestrous plasma estradiol level in ovary-intact female rats [19 (link), 29 (link)].

UENOYAMA Y., TANAKA A., TAKASE K., YAMADA S., PHENG V., INOUE N., MAEDA K.I, & TSUKAMURA H. (2015). Central estrogen action sites involved in prepubertal restraint of pulsatile luteinizing hormone release in female rats. The Journal of Reproduction and Development, 61(4), 351-359.

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Ovariectomized Rat Model for Estrogen Study

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Wistar female rats aged 8 weeks were purchased from Shimizu Laboratory Supplies Co. (Kyoto, Japan) and housed under a 12-h reverse light/dark cycle with free access to food and water. After two consecutive estrus cycles, rats were bilaterally ovariectomized and silastic tubing (1.5 mm i.d.; 3.0 mm o.d.; 25 mm length; Dow Corning, Midland, MI) containing crystalline 17 β-estradiol (E2, Nachalai, Osaka, Japan) was implanted subcutaneously under anesthesia with 2–3% isoflurane. We confirmed that the E2 treatment caused hypertrophy of the uterus and induced a high lordosis quotient (>90) against male mount behavior. All experimental procedures were authorized by the Committee for Animal Research, Kyoto Prefectural University of Medicine.

Yamada S, & Kawata M. (2014). Identification of neural cells activated by mating stimulus in the periaqueductal gray in female rats. Frontiers in Neuroscience, 8, 421.

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Surgical Implantation of Jugular Catheters in Rats

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Rats were anesthetized with ketamine HCl (males 87.5 mg/kg, IP; females 60 mg/kg, IP) and xylazine (males 5 mg/kg, IP; females 5 mg/kg, IP) in preparation for surgical implantation of jugular catheters (SILASTIC tubing, 0.51-mm inner diameter, 0.94 outer diameter, Dow Corning, Midland, MI, USA). The catheter exited the skin between the shoulder blades and attached to a stainless-steel cannula, which was held in place by a harness (Instech, Plymouth Meeting, PA, USA). Ketorolac (3 mg/kg, IP) was administered prior to surgery to provide analgesia, and for 2 days post-surgery, along with the antibiotic cefazolin (100 mg/mL, IV). Rats were allowed to recover for 5 days before the start of the experiment. During this time, and throughout the self-administration period, their catheters were flushed daily with 0.2 mL of heparinized saline (100 U/mL, Elkins-Sinn, Cherry Hill, NJ, USA). Catheter patency was verified periodically by intravenous administration of methohexital sodium (10 mg/mL, IV), which produces a temporary loss of muscle tone.

Bechard A.R., Hamor P.U., Schwendt M, & Knackstedt L.A. (2017). The effects of ceftriaxone on cue-primed reinstatement of cocaine-seeking in male and female rats: estrous cycle effects on behavior and protein expression in the nucleus accumbens. Psychopharmacology, 235(3), 837-848.

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Collars for Carotid Artery Manipulation in Mice

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Collars were prepared from Silastic tubing (Dow Corning) and were autoclaved until further use. Mice were anesthetized by intraperitoneal injection of ketamine/xylazine. The anterior cervical triangles were accessed through a sagittal anterior neck incision. The right carotid sheath was opened, and the common carotid artery was dissected from the surrounding connective tissue, avoiding damage to the vagus nerves and carotid bodies. Collars were placed bilaterally around the common carotid arteries, and their axial edges were approximated by placement of two circumferential silk ties. Subsequently, the skin incision was closed, and the animals were returned to their cage to recover from the anesthesia.

Zhang K., Zheng J., Chen Y., Dong J., Li Z., Chiang Y.P., He M., Huang Q., Tang H, & Jiang X.C. (2021). Inducible phospholipid transfer protein deficiency ameliorates atherosclerosis. Atherosclerosis, 324, 9-17.

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Surgical Implantation of Jugular Vein Catheter in Rats

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On the day of surgery, rats were anesthetized with isoflurane and implanted with a silastic catheter into the right external jugular vein using sterile surgical procedures as previously described by our laboratory (14 ). The catheter consisted of silastic tubing (0.025 inch inner diameter, 0.047 inch outer diameter; Dow Corning, Midland, MI,) connected to a metal guide cannula (22 gauge; Plastics One, Roanoke, VA) bent at a right angle. Catheters were flushed with heparinized saline (0.2 ml), routed to the back of the rat and sealed with a plastic cap and metal cannula cover. This catheter design allows for serial blood sampling over an extended period of time. Starting at 4 days post-surgery, catheters were flushed once daily with cefazolin (20 mg i.v.; Sigma-Aldrich, St. Louis, MO) in heparinized saline (20 U/ml, 0.1 ml total volume). Rats were allowed to recover for 1 week following surgery before initiating experimental procedures to allow stress hormone levels to return to baseline.

Whitaker A.M, & Gilpin N.W. (2015). Blunted Hypothalamo-pituitary Adrenal Axis Response to Predator Odor Predicts High Stress Reactivity. Physiology & behavior, 147, 16-22.

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Jugular Vein Catheterization Procedure

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Jugular catheters were made of 12 cm long silastic tubing (0.037 mm ID, 0.94 mm OD; Dow Corning) with small beads of 100% silicone rubber sealant at 8.5 and 9 cm, respectively. One end of the catheter was inserted into the right jugular vein and run subcutaneously below the front right leg to exit the back between the shoulder blades. A stainless steel guide cannula (22 ga; Plastics One) was inserted into an elastomer self-administration harness (Instech Laboratories) and the jugular catheter was attached to the cannula within the harness.

Pizzimenti C.L., Navis T.M, & Lattal K.M. (2017). Persistent effects of acute stress on fear and drug-seeking in a novel model of the comorbidity between post-traumatic stress disorder and addiction. Learning & Memory, 24(9), 422-431.

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