A gas chromatograph of the Thermo Electron type (Trace GC Ultra) and a mass spectrometer system of the Thermo Electron Trace MS type were used for the chromatographic examination of EOs (Thermo Electron: Trace Ultra GC, Polaris Q MS). The fragmentation was achieved with an electronic impact intensity of 70 eV. A DB-5 column (5% phenyl-methyl-siloxane) (30 m × 0.25 mm × 0.25 m film thickness) and a flame ionization detector (FID) powered by a mixture of He gas/air were installed in the chromatograph. For 5 min, the column temperature was designed to rise at a rate of 4 °C/min from 50 to 200 °C. The used carrier gas was nitrogen, flowing at a rate of 1 mL/min in the split injection mode (leak rate: 1/70). By comparing the essential oil compounds’ Kováts index (KI) and Adams to those of the reference products noted in the literature, the essential oil compounds were identified [57 (link),58 ]. Additionally, the mass spectra and indexes of each of these compounds were compared to those in the aforementioned databases [59 ]. The retention duration of any product is compared using the Kováts index to a linear alkane with the same carbon number.
Trace gc ultra
Manufactured by Thermo Fisher Scientific
Sourced in United States, Italy, Germany, United Kingdom, Belgium, Spain, Switzerland
The Trace GC Ultra is a gas chromatograph designed for trace-level analysis. It features high sensitivity and reproducibility, enabling accurate identification and quantification of trace-level compounds in complex samples. The Trace GC Ultra provides consistent and reliable performance for a wide range of applications.
Automatically generated - may contain errors
Lab products found in correlation
Polaris q, by Thermo Fisher Scientific (12 mentions)
Dsq 2, by Thermo Fisher Scientific (9 mentions)
Dsq 2 mass spectrometer, by Thermo Fisher Scientific (9 mentions)
Xcalibur 2, by Thermo Fisher Scientific (9 mentions)
Db ffap, by Agilent Technologies (8 mentions)
Xcalibur, by Thermo Fisher Scientific (7 mentions)
Itq 900, by Thermo Fisher Scientific (7 mentions)
Polaris q ms, by Thermo Fisher Scientific (7 mentions)
Masslynx 4.1 operating system, by Waters Corporation (6 mentions)
Tsq quantum xls, by Thermo Fisher Scientific (5 mentions)
Itq 1100, by Thermo Fisher Scientific (5 mentions)
Ctc combipal, by CTC Analytics (5 mentions)
Acquity uplc system, by Waters Corporation (5 mentions)
Isq mass spectrometer, by Thermo Fisher Scientific (4 mentions)
Xcalibur software, by Thermo Fisher Scientific (4 mentions)
Db 5 capillary column, by Agilent Technologies (4 mentions)
Supelcowax 10, by Merck Group (3 mentions)
Thermo isq mass spectrometer, by Thermo Fisher Scientific (3 mentions)
30 m tg 5ms column, by Thermo Fisher Scientific (3 mentions)
Triplus, by Thermo Fisher Scientific (3 mentions)
273 protocols using trace gc ultra
1
GC-MS Analysis of Essential Oils
2
Sheep Feed Composition Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Representative samples of the test feeds that supplied to the sheep were collected and stored at −20 °C. Immediately before the analysis, all the samples were dried and ground to pass through a 1-mm filter using a sample mill (Cemotec, Tecator, Sweden). The DM fraction was quantified by drying the samples at 60°C for 48 h until constant weight. The CP, ether extract (EE), and crude ash contents were determined by the AOAC method [8 ]. Neutral detergent fiber (NDF, heat-stable α-amylase), acid detergent fiber (ADF) were determined using the method of Van Soest et al. [9 (link)]. Ruminal NH3-N was determined according to Chaney and Marbach [10 (link)]. Ruminal VFA was determined according to Erwin et al. [11 (link)] using a Trace GC Ultra gas chromatograph (Trace GC Ultra, Thermo, Italy).
3
Gas Chromatographic Analysis of Essential Oils
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The chromatographic analysis of the essential oils from the aerial part of the two plants was carried out on a gas chromatograph of the Thermo Electron type (Trace GC Ultra) coupled to a mass spectrometer of the Thermo Electron Trace MS system type (Thermo Electron: Trace GC Ultra; Polaris Q MS), fragmentation was carried out by the electronic impact of intensity 70 eV. The chromatograph was equipped with a DB-5 (5% phenyl-methyl-siloxane) type column (30m × 0.25mm × 0.25μm film thickness), a flame ionization detector (FID) powered by a mixture of He gas/Air. The temperature of the column was programmed at a rate of a rise of 4 °C/min from 50 to 200 °C for 5 min. The injection mode was split (leakage ratio: 1/70, flow rate mL/min), the carrier gas used was nitrogen with a flow rate of 1 mL/min. The identification of the chemical composition of the essential oils of the two plants was carried out based on the comparison of their Kováts index (KI) and Adams with those of the reference products known in the literature [80 (link),81 ]. It was supplemented by a comparison of indices and mass spectra with different references [82 ,83 ]. The Kováts index compares the retention time of any product with that of a linear alkane of the same carbon number.
4
GC-MS Analysis of Essential Oils
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The analysis of the EOs’ chromatography was done using a gas chromatograph of the Thermo Electron type (Trace GC Ultra) coupled to a mass spectrometer of the Thermo Electron Trace MS system type (Thermo Electron: Trace GC Ultra; Polaris Q MS), where the fragmentation is done by electron impact with a 70 eV intensity. The chromatograph has a flame ionization detector (FID) driven by an H2/Air gas mixture and a DB-5 type column (5% phenyl-methyl-siloxane) measuring 30 m × 0.25 mm × 0.25 μm film thickness. For 5 min, the column temperature will rise at a rate of 4 °C/min from 50 to 200 °C. Split injection is employed, with a leakage ratio of 1/70 and a flow rate of 1 mL/min for the vector gas nitrogen. By comparing the calculated Kovats indices (IK) of EOs with those of Adams and other reference products that were known to exist in the literature [68 ,69 ], the chemical composition of EOs was identified. It was enhanced by comparing indices and mass spectra with other references [68 ].
5
GC-MS Analysis of Lavender Essential Oil
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The analysis of the lavender EO samples was carried out by a Thermo Electron type gas chromatograph (Trace GC Ultra) coupled to a Thermo Electron Trace MS system mass spectrometer (Thermo Electron: Trace GC Ultra; Polaris Q MS). Fragmentation is carried out by electronic impact with an intensity of 70 eV. The chromatograph is equipped with a DB-5 column (5% phenyl-methyl-siloxane) (30 m × 0.25 mm x 0.25 μm film thickness), and a flame ionization detector (FID) powered by a mixture of H2/Air gas. The column temperature is programmed at a rate of 4°C/min from 50°C to 200°C for 5 min. The device has a split–splitless PVT (Programmed Vaporization Temperature) injector. Split injection is employed, with a leakage ratio of 1/70 and a flow rate of 1 mL/min for the vector gas nitrogen.
The identification of the constituents of essential oils was made based on the determination and comparison of the Kovats indices (KI) of the compounds with those of the standard products known and described in the databases of Kovats (1965) and Adams (2007) , By conducting a comparison of the peak retention periods with the known legitimate standards present in the authors’ laboratory, as well as comparing the stated KI and MS data with the mass spectral database standards of WILEY and NIST 14, along with published literature.
The identification of the constituents of essential oils was made based on the determination and comparison of the Kovats indices (KI) of the compounds with those of the standard products known and described in the databases of Kovats (1965) and Adams (2007) , By conducting a comparison of the peak retention periods with the known legitimate standards present in the authors’ laboratory, as well as comparing the stated KI and MS data with the mass spectral database standards of WILEY and NIST 14, along with published literature.
6
GC-MS Analysis of Essential Oils
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The chromatographic analysis of EO samples was carried out using a Thermo-Electron-type gas chromatograph (Trace GC Ultra) coupled to a Thermo-Electron Trace MS system mass spectrometer (Thermo Electron: Trace GC Ultra; Polaris Q MS). Fragmentation is carried out by an electronic impact (70 eV intensity). The chromatograph is equipped with a DB-5 column (5% phenyl methyl-siloxane) (30m × 0.25mm × 0.25μm), a flame ionization detector (FID) supplied by a mixture of H2 / Air gas. The temperature of the column increases with a gradient of 4 ° C/min from 50 to 200 ° C. for 5 min. The injection mode is split (leak 1/70, flow rate ml/min), nitrogen is used as a carrier gas with a flow rate of 1 ml/min.
Identification of EO chemical composition was performed through the comparison of compounds' Kovats (IK) indices with those of standard products known from the literature (Kovats 1965, Adams, 2007) . This step was supplemented by a comparison of Kovats's indices of the compounds as well as their mass spectra with those gathered in reference documents (Adams, 2007 , National Institute of Standards and Technology, 2014). Kovats's indices compare the retention time of any product with the retention time of a linear alkane containing the same carbon number. They are determined by injecting a mixture of the alkanes (C7-C40 standard) under the same operating conditions.
Identification of EO chemical composition was performed through the comparison of compounds' Kovats (IK) indices with those of standard products known from the literature (Kovats 1965, Adams, 2007) . This step was supplemented by a comparison of Kovats's indices of the compounds as well as their mass spectra with those gathered in reference documents (Adams, 2007 , National Institute of Standards and Technology, 2014). Kovats's indices compare the retention time of any product with the retention time of a linear alkane containing the same carbon number. They are determined by injecting a mixture of the alkanes (C7-C40 standard) under the same operating conditions.
7
Carbon and Nitrogen Isotope Analysis of BAM
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Carbon
and nitrogen isotope values of BAM were measured on a GC-IRMS system.
A DB-5 analytical column (60 m, 0.25 mm i.d., 0.5 μm film, Agilent
Technologies, Germany) was used in a TRACE GC Ultra gas chromatograph
(Thermo Fisher Scientific, Italy) which was coupled to a Finnigan
MAT 253 isotope-ratio mass spectrometer through a Finnigan GC Combustion
III interface (Thermo Fisher Scientific, Germany). The typical total
uncertainty of carbon isotope measurements is ±0.5‰, and
the uncertainty of nitrogen isotope measurements is ±1‰.
The method, including solid-phase extraction before CSIA, is described
by Sun et al.54 (link) and provided in theSupporting Information .
and nitrogen isotope values of BAM were measured on a GC-IRMS system.
A DB-5 analytical column (60 m, 0.25 mm i.d., 0.5 μm film, Agilent
Technologies, Germany) was used in a TRACE GC Ultra gas chromatograph
(Thermo Fisher Scientific, Italy) which was coupled to a Finnigan
MAT 253 isotope-ratio mass spectrometer through a Finnigan GC Combustion
III interface (Thermo Fisher Scientific, Germany). The typical total
uncertainty of carbon isotope measurements is ±0.5‰, and
the uncertainty of nitrogen isotope measurements is ±1‰.
The method, including solid-phase extraction before CSIA, is described
by Sun et al.54 (link) and provided in the
8
GC-MS Analysis of Plant Extract Volatiles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The GC-MS system (GC-Thermo Trace GC Ultra, USA and ITQ 900 Mass Spec-Thermo, USA) was used to identify volatile chemicals in the EPL extracts. The plant extract was dissolved in methanol (MeOH) and subsequently purified using the QuEChERS method for solid-phase extraction [70 (link),71 (link)]. The GC-MS analysis was conducted employing a TG-SQC capillary column (30 m × 0.25 mm × 0.25 μm). High purity helium (99.99%) was employed as the carrier gas with a constant flow rate of 1 mL/min. A sample solution of 1 μL was injected with a split ratio of 10:1. The injector temperature was set at 250 °C, while the ion-source temperature was maintained at 230 °C. The column was initially held at a temperature of 50 °C for 2 min, followed by a gradual increase to 250 °C at a rate of 10 °C/min. Subsequently, the temperature was further increased to 280 °C and held for 10 min. MS data were acquired with an electron energy of 70 eV, a scanning interval time of 0.5 s, and fragments ranging from 35 to 650 Da. Compound identification was carried out by comparing them with reported compounds using data from the Mass Spectra Library (NIST 17 and Wiley) [54 (link),72 (link),73 ].
9
Direct Transmethylation and FAME Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fatty acid composition and content were determined as FA methyl esters (FAMEs), following the direct transmethylation of freeze-dried cell pellets and triacylglycerols, isolated from total lipid extracts. Samples placed in glass vials were transmethylated with 2% (v/v) sulfuric acid (H2SO4) in anhydrous methanol at 80°C for 1.5 h under an argon atmosphere with continuous stirring. Pentadecanoic acid (C15:0; Sigma-Aldrich, United States; 0.5 mg/ml in stock solution) was added as an internal standard. The transmethylation reaction was terminated by cooling to room temperature and the addition of 1 ml of H2O. FAMEs were extracted with hexane and quantified on a Trace GC Ultra (Thermo, Italy) equipped with an FID and a programmed temperature vaporizer (PTV) injector. The PTV injector was programmed to increase the temperature from 40°C at the time of injection to 300°C at sample transfer. The separation was achieved on a fused silica capillary column (SUPELCOWAX 10, Sigma-Aldrich, United States, 30 m × 0.32 mm) using the following oven temperature program: 1 min at 130°C, followed by a linear gradient to 220°C, and finally 10 min isocratic at 220°C. Helium was used as the carrier gas at a flow rate of 2.5 ml min−1. The detector temperature was set at 280°C. FAMEs were identified by co-chromatography with commercial standards (Sigma-Aldrich, United States).
10
Quantitative Characterization of Diterpenes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All chemicals were obtained from standard sources at the highest purity grade. NMR spectra were recorded in CDCl3 with an Avance-III 500 MHz (Bruker) at 300 K. 1H NMR chemical shifts are given in ppm relative to CHCl3 (δ = 7.26 ppm). 13C NMR chemical shifts are given in ppm relative to CDCl3 at δ = 77.16. The 2D experiments (HSQC, COSY, NOESY) were performed using standard Bruker pulse sequences and parameters.
GC-MS analysis of diterpene products from ethyl acetate extractions was done by a Trace GC Ultra with DSQII (Thermo Scientific). One microliter sample was applied by TriPlus AS onto a SGE BPX5 column (30 m, I.D 0.25 mm, Film 0.25 μm). The initial column temperature was 50°C (maintained for 2.5 min). A temperature gradient was applied from 50 to 320°C (10°C/min), followed by 3 min maintenance at 320°C. MS data were recorded at 70 eV (EI), m/z (rel. intensity in %) as TIC, total ion current. The recorded m/z range was 50–650. Quantification was performed with flame ionization detector (FID) using 1 mg/mL α-humulene (Sigma–Aldrich, Germany) as an internal standard.
GC-MS analysis of diterpene products from ethyl acetate extractions was done by a Trace GC Ultra with DSQII (Thermo Scientific). One microliter sample was applied by TriPlus AS onto a SGE BPX5 column (30 m, I.D 0.25 mm, Film 0.25 μm). The initial column temperature was 50°C (maintained for 2.5 min). A temperature gradient was applied from 50 to 320°C (10°C/min), followed by 3 min maintenance at 320°C. MS data were recorded at 70 eV (EI), m/z (rel. intensity in %) as TIC, total ion current. The recorded m/z range was 50–650. Quantification was performed with flame ionization detector (FID) using 1 mg/mL α-humulene (Sigma–Aldrich, Germany) as an internal standard.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!