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Anti tef4 tead2

Manufactured by Novus Biologicals
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Anti-TEF4 (Tead2) is a research antibody product used to detect the transcriptional enhancer factor 4 (Tead2) protein in biological samples. It can be utilized in various techniques such as Western blotting, immunohistochemistry, and immunocytochemistry to study the expression and localization of Tead2 in cells and tissues.

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Lab products found in correlation

Anti β actin, by Santa Cruz Biotechnology (2 mentions) Dual luciferase reporter assay kit, by Promega (2 mentions) Anti yap1, by Cell Signaling Technology (2 mentions)

2 protocols using anti tef4 tead2

1

Transcriptional Regulation by HNF4A and TEAD

Cited 1 time
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HNF4A and HNF4A DN expression vectors were provided by T. Leff (Hong et al., 2003 (link)). TEAD2 and YAP1 expression vectors were provided by J.F. Martin (Heallen et al., 2011 (link)). TEAD DN and YAP S94A expression vectors were previously described (Shen et al., 2012 (link)). Enhancer regions were PCR amplified and cloned into a PGL3 basic vector containing the E1B minimal promoter. TK-Renilla controlled for transfection efficiency. HEK293T were maintained in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum. Transfections were performed using PEI (Raju et al., 2013 (link)). Firefly and Renilla activity was measured with a Dual Luciferase Reporter Assay kit (Promega) and presented as the Firefly/Renilla ratio. Western blot analysis was carried out on nuclear extracts from pooled embryonic livers (E14.5) and female adult liver using anti-YAP1 (Cell Signaling), anti-TEF4 (Tead2; Novus Biologicals), and anti-beta actin (Santa Cruz). Nuclear extracts for EMSAs were prepared from 293T cells transfected with TEAD-myc/HNF4-myc or E14.5 as previously described (Labbé et al., 1998 (link)).
Alder O., Cullum R., Lee S., Kan A.C., Wei W., Yi Y., Garside V.C., Bilenky M., Griffith M., Morrissy A.S., Robertson G.A., Thiessen N., Zhao Y., Chen Q., Pan D., Jones S.J., Marra M.A, & Hoodless P.A. (2014). Hippo Signaling Influences HNF4A and FOXA2 Enhancer Switching during Hepatocyte Differentiation. Cell reports, 9(1), 261-271.
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2

Transcriptional Regulation by HNF4A and TEAD

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
HNF4A and HNF4A DN expression vectors were provided by T. Leff (Hong et al., 2003 (link)). TEAD2 and YAP1 expression vectors were provided by J.F. Martin (Heallen et al., 2011 (link)). TEAD DN and YAP S94A expression vectors were previously described (Shen et al., 2012 (link)). Enhancer regions were PCR amplified and cloned into a PGL3 basic vector containing the E1B minimal promoter. TK-Renilla controlled for transfection efficiency. HEK293T were maintained in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum. Transfections were performed using PEI (Raju et al., 2013 (link)). Firefly and Renilla activity was measured with a Dual Luciferase Reporter Assay kit (Promega) and presented as the Firefly/Renilla ratio. Western blot analysis was carried out on nuclear extracts from pooled embryonic livers (E14.5) and female adult liver using anti-YAP1 (Cell Signaling), anti-TEF4 (Tead2; Novus Biologicals), and anti-beta actin (Santa Cruz). Nuclear extracts for EMSAs were prepared from 293T cells transfected with TEAD-myc/HNF4-myc or E14.5 as previously described (Labbé et al., 1998 (link)).
Alder O., Cullum R., Lee S., Kan A.C., Wei W., Yi Y., Garside V.C., Bilenky M., Griffith M., Morrissy A.S., Robertson G.A., Thiessen N., Zhao Y., Chen Q., Pan D., Jones S.J., Marra M.A, & Hoodless P.A. (2014). Hippo Signaling Influences HNF4A and FOXA2 Enhancer Switching during Hepatocyte Differentiation. Cell reports, 9(1), 261-271.
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