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Insulin novolin

Manufactured by Novo Nordisk
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Sourced in Japan

Insulin (Novolin) is a synthetic form of the hormone insulin, which is produced by the pancreas and plays a crucial role in regulating blood sugar levels. Insulin (Novolin) is used to manage diabetes by helping the body effectively use and store glucose.

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Lab products found in correlation

Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (4 mentions) Succinylacetone, by Merck Group (2 mentions) Mannitol, by Merck Group (2 mentions) Isoproterenol, by Merck Group (2 mentions) 8 br camp, by Merck Group (2 mentions) Protoporphyrin 9, by Merck Group (2 mentions) Cl 316 243, by Cayman Chemical (2 mentions) Forskolin, by Chem-Impex (2 mentions) Gibco branded cell culture products, by Thermo Fisher Scientific (2 mentions) On target sirna smartpools, by Horizon Discovery (2 mentions) Hemin, by Merck Group (2 mentions) Hek293t cell, by American Type Culture Collection (2 mentions) Oligomycin a, by Merck Group (2 mentions) Antimycin a, by Merck Group (2 mentions) Norepinephrine, by Merck Group (2 mentions) Complete edta free protease inhibitor cocktail, by Roche (2 mentions) Igf 1, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Nacalai Tesque (1 mentions) Mouse c2c12 myoblast, by American Type Culture Collection (1 mentions)

Close spelling variants of this product

Insulin (160 citations) Novolin (69 citations) Novolin-R insulin (4 citations) Novolin R regular insulin (3 citations) Novolin 70:30 insulin (2 citations)

3 protocols using insulin novolin

1

Heme Regulation of Cellular Metabolism

Cited 1 time
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Hemin, protoporphyrin IX, oligomycin A, carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP), rotenone, antimycin A, 3-isobutyl-1-methylxanthine (IBMX), BSA, mannitol, norepinephrine, isoproterenol, 8-Br-cAMP, and succinylacetone were purchased from Sigma-Aldrich. CL 316,243 was obtained from Cayman Chemical. Forskolin was obtained from Chem Impex International. Insulin (Novolin) was purchased from Novo-Nordisk. Complete EDTA-free protease inhibitor cocktail was obtained from Roche. DMEM and other Gibco-branded cell culture products were purchased from Thermo Fisher. Heme-depleted FBS was prepared by treating FBS with 20 mM ascorbic acid for 16 hr, followed by 24 hr dialysis against PBS. Heme depletion was verified by measuring optical absorbance at 405 nm. CPAG-1 was synthesized as previously reported5 (link). ON-TARGET siRNA SMARTpools against human PGRMC2 (L-010639-00-0005), PGRMC1 (L-010642-00-0005), and mouse Nr1d1 (L-051721-00-0005), and BACH1 (L-042956-01-0005), as well as a Non-targeting Pool (D-001810-10-05) were purchased from Dharmacon. HEK293T cells were obtained from ATCC (CRL-3216) having undergone short-tandem repeat verification. Cells were routinely tested for mycoplasma and were never positive.
Galmozzi A., Kok B.P., Kim A.S., Montenegro-Burke J.R., Lee J.Y., Spreafico R., Mosure S., Albert V., Cintron-Colon R., Godio C., Webb W.R., Conti B., Solt L.A., Kojetin D., Parker C.G., Peluso J.J., Pru J.K., Siuzdak G., Cravatt B.F, & Saez E. (2019). PGRMC2 is an Intracellular Heme Chaperone Critical for Adipocyte Function. Nature, 576(7785), 138-142.
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2

Heme Regulation of Cellular Metabolism

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Hemin, protoporphyrin IX, oligomycin A, carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP), rotenone, antimycin A, 3-isobutyl-1-methylxanthine (IBMX), BSA, mannitol, norepinephrine, isoproterenol, 8-Br-cAMP, and succinylacetone were purchased from Sigma-Aldrich. CL 316,243 was obtained from Cayman Chemical. Forskolin was obtained from Chem Impex International. Insulin (Novolin) was purchased from Novo-Nordisk. Complete EDTA-free protease inhibitor cocktail was obtained from Roche. DMEM and other Gibco-branded cell culture products were purchased from Thermo Fisher. Heme-depleted FBS was prepared by treating FBS with 20 mM ascorbic acid for 16 hr, followed by 24 hr dialysis against PBS. Heme depletion was verified by measuring optical absorbance at 405 nm. CPAG-1 was synthesized as previously reported5 (link). ON-TARGET siRNA SMARTpools against human PGRMC2 (L-010639-00-0005), PGRMC1 (L-010642-00-0005), and mouse Nr1d1 (L-051721-00-0005), and BACH1 (L-042956-01-0005), as well as a Non-targeting Pool (D-001810-10-05) were purchased from Dharmacon. HEK293T cells were obtained from ATCC (CRL-3216) having undergone short-tandem repeat verification. Cells were routinely tested for mycoplasma and were never positive.
Galmozzi A., Kok B.P., Kim A.S., Montenegro-Burke J.R., Lee J.Y., Spreafico R., Mosure S., Albert V., Cintron-Colon R., Godio C., Webb W.R., Conti B., Solt L.A., Kojetin D., Parker C.G., Peluso J.J., Pru J.K., Siuzdak G., Cravatt B.F, & Saez E. (2019). PGRMC2 is an Intracellular Heme Chaperone Critical for Adipocyte Function. Nature, 576(7785), 138-142.
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3

Myogenic Differentiation of C2C12 Cells

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Mouse C2C12 myoblasts (American Type Culture Collection, Manassas, VA) were cultured as described previously (Yokokawa et al. 2015). Briefly, cells were grown in Dulbecco's modified Eagle's medium (DMEM; 4.5 g glucose/L, Nacalai Tesque, Kyoto, Japan) containing 10% fetal bovine serum and 1% penicillin‐streptomycin (P/S). To initiate myogenic differentiation, the culture medium was replaced by DMEM containing 2% horse serum and 1% P/S. After 4 days of differentiation, myotubes were serum‐starved overnight and then incubated for 30 min in serum‐free medium containing 5 μmol/L 5‐aminoimidazole‐4‐carboxamide ribonucleoside (AICAR; Wako, Osaka, Japan), 1 μmol/L insulin (Novolin; Novo Nordisk, Bagsvaerd, Denmark), or 200 ng/mL IGF1 (PeproTech, Rocky Hill, NJ).
Kido K., Ato S., Yokokawa T., Makanae Y., Sato K, & Fujita S. (2016). Acute resistance exercise‐induced IGF1 expression and subsequent GLUT4 translocation. Physiological Reports, 4(16), e12907.
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