Using commercially available diagnostic kits, the levels of serum glucose, insulin, pyruvic transaminase (GPT), glutamic oxaloacetic transaminase (GOT), creatinine, urea, total cholesterol (TC), total triglycerides (TG), low-density lipoprotein (LDL), and high-density lipoprotein (HDL) were measured (Human, Wiesbaden, Germany). Also, using rat ELISA kits, the levels of pro-inflammatory biomarkers such as TNF-α, IL-1β, IL-6, caspase-3, NO, and PGE-2 were determined according to the provided guidelines (R&D Systems Inc., USA). The physiological lysis buffer was used to homogenize the liver and kidney tissues (1:10, w/v). Diagnostic kits were used to measure the concentrations of TBARS and GSH (Cayman Chemical Co., USA). The enzymatic activity of SOD, CAT, GPx, GR, and GST in post-mitochondria supernatants of hepatic and renal tissues was assessed using assay kits (R&D Systems Inc., USA). TNF-α, IL-1β, and IL-6 levels were also assessed in hepatic and renal-prepared tissue samples using rat ELISA kits according to the provided protocol (R&D Systems Inc., USA).
Rat elisa kit
Manufactured by R&D Systems
Sourced in United States
The Rat ELISA kit is a laboratory tool designed to detect and quantify target analytes in rat biological samples using the enzyme-linked immunosorbent assay (ELISA) method. The kit provides the necessary reagents and protocols to perform this analysis.
Automatically generated - may contain errors
Lab products found in correlation
Rat elisa kit, by Cusabio (2 mentions)
Glutathione (gsh), by Cayman Chemical (1 mentions)
Tbars, by Cayman Chemical (1 mentions)
Rat elisa kits, by Sunlong Biotech (1 mentions)
High sensitive enzyme linked immunosorbent assay kit, by Cloud-Clone (1 mentions)
Interleukin 6 (il 6), by R&D Systems (1 mentions)
Tnf α, by R&D Systems (1 mentions)
Automatic analyzer 7600 210, by Hitachi (1 mentions)
Rat elisa kit, by Elabscience (1 mentions)
Cobas integra 400 plus, by Roche (1 mentions)
Advia 2400, by Siemens (1 mentions)
Rat elisa kit, by Thermo Fisher Scientific (1 mentions)
Ma5 27688, by Thermo Fisher Scientific (1 mentions)
Horseradish peroxidase labeled secondary antibody, by Jackson ImmunoResearch (1 mentions)
Anti nitrotyrosine, by Abcam (1 mentions)
Histoquest analysis software, by TissueGnostics (1 mentions)
Anti vwf antibody, by Agilent Technologies (1 mentions)
3 amino 9 ethylcarbazole, by Zymo Research (1 mentions)
Lps escherichia coli serotype 0111 b4, by Merck Group (1 mentions)
Streptavidin hrp conjugated secondary antibody, by Biocare Medical (1 mentions)
30 protocols using rat elisa kit
1
Metabolic and Inflammatory Biomarkers Analysis
2
Comprehensive Liver Injury Biomarker Assay
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Liver homogenates were assayed for cAMP using specific Rat ELISA kit (R&D Systems, USA; Catalogs Number: KGE012B).
Tumor necrosis factor (TNF-α), interleukin-6 (IL-6), and nuclear factor-kappa B (NF-κB) as important signals in liver injury [29 ], were also assayed, as well as transforming growth factor-beta (TGF-β) as a fibrogenesis-driving cytokine [30 ], using specific Rat ELISA kits (SunLong Biotech Co., LTD, China; Catalogs Number: SL0722Ra, SL0411Ra, SL0537Ra, and SL0705Ra, respectively).
As caspase-3 is involved in the apoptosis of hepatocytes during liver injury [31 ], CUSABIO Rat Casp-3 ELISA Kit (USA; Catalog Number: CSB-E08857r) was used to measure the level of caspase-3 in liver homogenate. Moreover, evaluation of α-smooth muscle actin (α-SMA) and type I collagen (COL-1) as classic liver fibrosis markers was also performed using specific Rat ELISA kits (CUSABIO, USA; Catalogs Number: CSB-E 14407r and CSB-E10134r, respectively).
Tumor necrosis factor (TNF-α), interleukin-6 (IL-6), and nuclear factor-kappa B (NF-κB) as important signals in liver injury [29 ], were also assayed, as well as transforming growth factor-beta (TGF-β) as a fibrogenesis-driving cytokine [30 ], using specific Rat ELISA kits (SunLong Biotech Co., LTD, China; Catalogs Number: SL0722Ra, SL0411Ra, SL0537Ra, and SL0705Ra, respectively).
As caspase-3 is involved in the apoptosis of hepatocytes during liver injury [31 ], CUSABIO Rat Casp-3 ELISA Kit (USA; Catalog Number: CSB-E08857r) was used to measure the level of caspase-3 in liver homogenate. Moreover, evaluation of α-smooth muscle actin (α-SMA) and type I collagen (COL-1) as classic liver fibrosis markers was also performed using specific Rat ELISA kits (CUSABIO, USA; Catalogs Number: CSB-E 14407r and CSB-E10134r, respectively).
3
Quantification of Chemokines in Lung Homogenate
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The lung tissue homogenate was centrifuged and the supernatant was used to determine the concentrations of Monocyte chemoattractant protein (MCP)-1, macrophage inflammatory protein (MIP)-2, and cytokine-induced neutrophil chemoattractant (CINC)-1 by a rat ELISA kit (R&D, Minneapolis, MN, USA) according to the manufacturer instructions.
4
Quantifying Surfactant Protein A in Lung Tissue
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The lung tissue homogenate was centrifuged and the supernatant was used to determine the concentration of SP-A by a rat ELISA kit (R&D, USA) according to the manufacturer instructions.
5
Cytokine and Cardiac Injury Biomarkers
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Blood samples from the recipient rats were obtained at 1.5 h after reperfusion just before the sacrifice of rats. Blood samples were drawn from the abdominal aorta. Then, plasma samples were obtained after centrifugation (3000 rpm, 15 min, 4 °C). The levels of proinflammatory cytokines (tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6) and cardiac troponin I (cTnI) were measured via Rat ELISA kit (R&D Systems, Inc., Minneapolis, MN, USA).
6
Inflammatory Cytokine Quantification
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The commercial rat ELISA kit (R&D Systems, Minneapolis, MN, USA) was used to evaluate TNF-α whereas for IL-6, the Cloud-Clone Corp. (Houston, TX, USA) High Sensitive Enzyme-linked Immunosorbent Assay Kit (HEA079Ra, Houston, TX, USA) was used.
These markers were measured according to the manufacturer’s instructions. TNF-α and IL-6 concentrations were determined from the standard curves and expressed in pg/mg protein.
These markers were measured according to the manufacturer’s instructions. TNF-α and IL-6 concentrations were determined from the standard curves and expressed in pg/mg protein.
7
Analysis of Inflammatory Markers in BALF
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The bronchoalveolar lavage fluid (BALF) was obtained by lavaging the left lung (2×) with 2.5 ml of saline after the experiment. The BALF was centrifuged at 200 × g for 10 min at room temperature. BALF protein concentration was measured using a bicinchoninic acid protein assay kit (Pierce, Rockford, IL, USA). BALF tumor necrosis factor-α (TNF-α, catalog number: RTA00), cytokine-induced neutrophil chemoattractant-1(CINC-1, catalog number: RCN100, also termed chemokine (C-X-C motif) ligand 1 [CXCL1]), and interleukin-6 (IL-6, catalog number: R6000B) levels were determined using a commercial rat ELISA kit (R&D Systems Inc., Minneapolis, MN, USA). BALF total cell counts were assessed as described previously (Wu et al., 2015 (link)).
8
Rat Serum IFN-γ Quantification
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Blood was drawn via a heart stick with a 3 mL syringe, and after allowing 30 min for clotting, the blood was centrifuged at 3000 rpm for 3 min. Rat serum was examined for IFN-γ using a rat ELISA kit (R&D system, MN).
9
Assessing IL-17A in Rat Retina and Serum
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The level of IL-17A in the retina and serum was assessed using a rat ELISA kit (R&D Systems, Minneapolis, MN, United States) as directed by the manufacturer (Zhu et al., 2022 (link)).
10
Comprehensive Metabolic and Inflammatory Profiling
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The serum levels of aspartate aminotransferase (AST), alkaline phosphatase (ALP), alanine aminotransferase (ALT), bilirubin, lactate dehydrogenase (LDH), albumin, glucose, triglyceride, total cholesterol, and low density lipoprotein (LDL) cholesterol were determined using a Hitachi automatic analyzer 7600-210 (Hitachi High-Technologies Corporation, Tokyo, Japan). The serum concentration of tumor necrosis factor-alpha (TNF-α), interleukin-1β (IL-1β) and TGF-β1 were measured by using a commercially available rat ELISA kit (R&D Systems, Minneapolis, MN, USA) following manufacturer’s instruction.
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