Resveratrol was purchased from Merck KGaA (Darmstadt, Germany), and it was dissolved in dimethyl sulfoxide (DMSO) to form a 10 mM stock solution. CCK8 kit and Annexin V-FITC/PI kit were obtained from Sigma-Aldrich. Dulbecco’s Modified Eagle Medium was purchased from HyClone and fetal bovine serum (FBS) was purchased from Gibco. Primary antibodies against cleaved-caspase-3 (dilution 1:1000), pro-caspase-3 (dilution 1:1000), Bcl-2 (dilution 1:1000), and β-actin (dilution 1:1000) were purchased from Abcam. PolyFect transfection reagent was obtained from Hilden (Qiagen, Germany). Dual-Luciferase® Reporter Assay System was purchased from Mannheim (Promega, Germany).
Primary antibodies against cleaved caspase 3
Manufactured by Abcam
Sourced in United States
Primary antibodies against cleaved-caspase-3. These antibodies bind to the activated form of the caspase-3 protein, which is a key executioner of apoptosis.
Automatically generated - may contain errors
Lab products found in correlation
Annexin 5 fitc pi kit, by Merck Group (1 mentions)
Cck 8 kit, by Merck Group (1 mentions)
Polyfect transfection reagent, by Qiagen (1 mentions)
Pro caspase 3, by Abcam (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dual luciferase reporter assay system, by Promega (1 mentions)
β actin, by Abcam (1 mentions)
Bcl 2, by Abcam (1 mentions)
Resveratrol, by Merck Group (1 mentions)
Chemiluminescence reagent, by Merck Group (1 mentions)
Chemigenius bioimaging system, by Syngene (1 mentions)
Anti rabbit peroxidase conjugated secondary antibodies, by Vector Laboratories (1 mentions)
β actin antibody, by Merck Group (1 mentions)
Fitc conjugated secondary antibody, by Abcam (1 mentions)
Hrp conjugated secondary antibody, by Abcam (1 mentions)
3 protocols using primary antibodies against cleaved caspase 3
1
Resveratrol-Induced Apoptosis Regulation
2
Cleaved Caspase-3 Immunoblotting in HT-22 Cells
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Supernatant treated HT-22 cells were harvested for obtaining total cellular extracts, and the protein isolation procedure and immunoblotting steps were performed according to standard procedure. After being blocked with 5% skimmed milk, the membranes were incubated with primary antibodies against cleaved caspase-3 (Abcam, USA), at 1:1,000 dilutions. After extensive washes with 0.1% PBS-Tween, blots were incubated with the Anti-Rabbit peroxidase-conjugated secondary antibodies (Vector Laboratories, USA). The blots were processed for development using chemiluminescence reagent (Millipore, USA). The images were captured and analyzed using the Chemigenius bioimaging system (Syngene, United Kingdom). β-actin antibody (Sigma, USA) at 1:10,000 dilution was used as loading control.
3
Apoptosis and ER Stress Markers
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Primary antibody directed against GAPDH (catalog number ab9485) was purchased from Abcam (Cambridge, MA, USA); primary antibodies against Cleaved Caspase 3 (catalog number BF0711), Cleaved Caspase 9 (catalog number AF5240), Bax (catalog number AF0120), Bcl-2 (catalog number AF6139), ATF6 (catalog number DF6009), CHOP (catalog number DF6025), Phospho-PERK (catalog number DF7576), PERK (catalog number AF4799), Phospho-IRE1 (catalog number AF7150), IRE1 (catalog number DF7709), Phospho-JNK (catalog number AF3318) and JNK (catalog number AF6318) were obtained from A nity (Cincinnati, OH, USA). HRP-conjugated secondary antibodies (catalog numbers ab6721 and ab6885) and a FITC-conjugated secondary antibody (catalog number ab6717) were purchased from Abcam (Cambridge, MA, USA).
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