Bcl 2 ab59348

Spermidine (S0266), 3‐methyladenine (M9281), TBHP (458139) and type II collagenases (1148090) were purchased from Sigma‐Aldrich (St Louis, MO, USA). p62 (ab56416), LC‐3(ab128025), Beclin‐1(ab6242), Atg7(ab52472), cleaved caspase‐3(ab32042), Bax(ab32503) and Bcl‐2(ab59348) antibodies were purchased from Abcam (Cambridge, UK). FITC‐labelled and horseradish peroxidase‐labelled secondary antibodies were purchased from Abcam (ab7086, ab191866, ab193651). 4′, 6‐diamidino‐2‐phenylindole (DAPI) was obtained from Beyotime (C1002, Shanghai, China).

TG (10522) and PBA (P21005) were purchased from Cayman Chemicals (Ann Arbor, MI, USA) and Sigma-Aldrich (Saint Louis, MO, USA), respectively. Western blot antibodies, including BiP (3183), CHOP (5554), Caspase-3 (9662), Bax (2772), TNFα (3707), p.JNK (4671), T.JNK (9252), p.P38 (9211), T. P38 (8690), p.IRS1-ser307 (2381), T.IRS (2382), β-Actin (4970), and secondary HRP conjugated (7074), were purchased from Cell Signaling Technology (Danvers, MA, USA), and Bcl-2 (ab59348) was obtained from Abcam (Waltham, MA, USA). The Amersham ECL select (RPN2235) reagent kit, Halt™ Protease and Phosphatase Inhibitor Cocktail (78440) and PVDF membrane (IPVH00010) were purchased from GE Health Care and Millipore, respectively. PCR materials were YTzol (Yekta Tajhiz Azma, Tehran, Iran, #YT9065), total RNA isolation kit (RiboEX GeneAll, Seoul, South Korea), 301-902), cDNA Synthesis Kit (Yekta Tajhiz Azma, Tehran, Iran, #YT4500) and SYBR Green Real-Time PCR Master Mix (2×) (Ampliqon, Copenhagen, Denmark).

BPA (CAS no. 80-05-7) was from Sigma Chemical Company (Saint Louis, MO, USA). Rabbit anti-CYP11A1 (ab175408), 3β-HSD (ab65156), StAR (ab203193), Bcl-2 (ab59348), and Bax (ab53154) primary antibody and secondary antibody anti-rabbit were from Abcam (Cambridge, MA). The DeadEndTM Colorimetric TUNEL System was from Promega Corporation (Promega, Madison, WI, USA).