Nogo a

Manufactured by Merck Group

Sourced in Morocco

Nogo-A is a laboratory product developed by Merck Group. It is a protein that plays a role in the inhibition of axon regeneration in the central nervous system. The core function of Nogo-A is to serve as a research tool for studying neural plasticity and regeneration in various neurological conditions.

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Lab products found in correlation

Ab84125, by Abcam (2 mentions) Ab22884, by Abcam (2 mentions) Ab17901, by Abcam (2 mentions) Ab60229, by Abcam (2 mentions) Ab36593, by Abcam (2 mentions) Glial fibrillary acidic protein (gfap), by Merck Group (2 mentions) P75ntr, by Merck Group (1 mentions) Anti bdnf, by Abcam (1 mentions) P jnk, by Santa Cruz Biotechnology (1 mentions) Ubiquitin, by Santa Cruz Biotechnology (1 mentions) Cofilin, by Abcam (1 mentions) Nedd4, by Abcam (1 mentions) Glua1, by Merck Group (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) β actin, by Merck Group (1 mentions) Perfect block, by MoBiTec (1 mentions) Las 4000, by Fujifilm (1 mentions)

4 protocols using nogo a

Western Blot Analysis of Brain Protein Markers

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Brains were homogenized and processed for western blotting as previously described [27 (link)]. Thirty μg of proteins were loaded in each lane and separated followed by transfer to nitrocellulose membranes. The membranes were blocked using 5% non-fat milk in TBST (1% tween 20 in tris buffered saline) and probed with the following antibodies antiBDNF (1:250; abcam; Cambridge, MA), TrkB (1:500, abcam; Cambridge, MA), p75NTR (1:1000, Millipore; Billerica, MA), Nogo-A (1:1000, Millipore; Billerica, MA), CHOP (1:1000) and nNOS (1:1000, Cell Signaling; Danvers, MA), ATF6 (1:500), pJNK (1:1000) and JNK (1:1000, Santa Cruz biotechnologies; Santa Cruz, CA). Expression was assessed by quantification of optical density of respective bands normalized to actin using NIH-image J software.

Alhusban A., Kozak A., Pillai B., Ahmed H., Sayed M.A., Johnson M.H., Ishrat T., Ergul A, & Fagan S.C. (2017). Mechanisms of acute neurovascular protection with AT1 blockade after stroke: Effect of prestroke hypertension. PLoS ONE, 12(6), e0178867.

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Protein Expression Profiling in Cellular Samples

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The primary antibodies used were as follows: APC (1:100 mouse monoclonal OP80, Calbiochem), Lef1 (1:200 Rabbit mAb C12A5, Cell Signaling), anti-human Lef1 (1:100 rabbit polyclonal Ab22884, Abcam), RNF43 (1:200 rabbit polyclonal Ab84125, Abcam), ETS2 (1:200 chicken polyclonal Ab17901, Abcam), DUSP4 (1:200 rabbit polyclonal Ab60229, Abcam), SP5 (1:200 rabbit polyclonal Ab36593, Abcam), Tcf4 (1:100 mouse monoclonal 6H5-3, Upstate), Olig2 (1:3000 rabbit polyclonal from CD Stiles, Harvard), Nkx2.2 (1:100 mouse monoclonal, Developmental Studies Hybridoma Bank), PDGFRα (1:200 rat 558774, BD Biosciences), NOGO-A (1:200 rabbit, Millipore), and GFAP (1:500 mouse, Sigma).

Fancy S.P., Harrington E.P., Baranzini S.E., Silbereis J.C., Shiow L.R., Yuen T.J., Huang E.J., Lomvardas S, & Rowitch D.H. (2014). Parallel states of pathological Wnt signaling in neonatal brain injury and colon cancer. Nature neuroscience, 17(4), 506-512.

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Western Blot Analysis of Neuronal Proteins

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Samples were processed using 10% acrylamide gels and transferred to PVDF membranes. Membranes were blocked with 1% blocking buffer (Perfect-block; MoBiTec) in TBS-TritonX (0.1%) for 1 h and incubated overnight with a primary antibody against Nogo-A 1 : 1000 (Millipore), MAG 1:1000 (Cell Signaling Technology), OMgp 1:4000 (Millipore), Troy 1:500 (R&D Systems), NgR 1:1000 (R&D Systems), phospho-S3cofilin 1:1000 (Abcam: ab12866), cofilin 1:1000 (Abcam: ab42824), GluA1 1:1000 (Millipore; clone C3T), NEDD4 1:4000 (Abcam; ab14592), phospho-S845GluA1 1:1000 (Millipore), phospho-S831GluA1 1:1000 (Millipore), β-actin 1:2000 (Sigma), GAPDH 1:2000 (Cell Signaling Technology), or Ubiquitin 1:200 (Santa Cruz: sc8017) at 4°C. Blots were then washed in TBS-TritonX and placed in HRP-conjugated anti-rabbit or anti-mouse secondary antibody at a 1:1000 dilution. Blots were then washed and reacted with electrochemiluminescence (ECL) or ECL-prime reagents. ECL-treated blots were quantified by densitometry using LAS4000 (Fujifilm). Protein phosphorylation data are expressed as percentages of the control.

Jitsuki S., Nakajima W., Takemoto K., Sano A., Tada H., Takahashi-Jitsuki A, & Takahashi T. (2015). Nogo Receptor Signaling Restricts Adult Neural Plasticity by Limiting Synaptic AMPA Receptor Delivery. Cerebral Cortex (New York, NY), 26(1), 427-439.

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Protein Expression Profiling in Cellular Samples

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